Preparation of an enzymatically active formulation embedded in silica gel

Claim 2

A process for the preparation of an enzymatically active formulation embedded in silica gel which comprises: suspending an aqueous mixture consisting of an enzymatically active formulation and a dissolved alkali metal silicate and/or ammonium silicate as droplets in a stirred organic, water-immiscible fluid and then converting said silicate to a water-insoluble gel.

Claim 3

A process as claimed in claim 1, wherein the enzymatically active formulation used consists of active cells or cell fragments of microbiological, vegetable, animal or human origin.

Claim 4

A process set forth in claim 1, wherein the formation of a suspension is assisted by using a suspending agent.

Claim 5

A process as set forth in claim 1, 2 or 3, wherein the gelling is effected by lowering the pH by means of an agent which is soluble in water and in the organic water-immiscible fluid.

Claim 6

A process of claim 4 wherein said agent is an organic acid.

Claim 7

A process as set forth in claim 1, wherein the gelling is effected in the presence of an inert material.

Claim 8

A process as claimed in claim 1, wherein said enzymatically active formulation embody as micro-organisms one of Streptomyces, Arthrobacter or Bacillus microorganisms, or Escherichia coli, Saccharomuces, Curvularia lunata, or Aspergillus ochraceus.

Claim 9

A process as claimed in claim 1, wherein the enzymatically active formulation embody of one of trypsin, chymotrypsin, pancreatin, α- and β-amylase, ribonucleases, desoxyribonucleases, cellulase, maltase, pectinase, chitinase, pepsin, bromelain, keratinase, amyloglycosidase, lipase, cholinesterase, lecithinase, phosphatase, alginase, asparaginase, glutaminase, urease, lactase, penicillin-amidase, penicillinase, glucose-isomerase, glucose-oxidase, catalase, peroxidase, lipoxidase, xanthin-oxidase, cytochrome-reductase, lactic acid oxidase, aminoacid oxidase, rennin, ficin, subtilisin, tannase, phenol-oxidase, pullulanase, isoamylase, hexokinase, galactose-oxidase, diaphorase, aldolase, glycollic acid oxidase, luciferase, aldehyde-oxidase, naringinase, uricase, glutathione-reductase, nitrito-reductase, nitrate-reductase, succinic acid dehydrogenase, catechol-oxidase, β-fructosidase, aminoacid acylase and urokinase.

Claim 10

A process as claimed in claim 1, wherein said enzymatically active formulation comprises baker's yeast.

Claim 11

A process as claimed in claim 1, wherein the embedded enzymatic formulation is immobilized by the embedding thereof in said gel.

Claim 12

A process for the preparation of an enzymatically active formulation embedded in silica gel which comprises: suspending an aqueous mixture consisting of an enzymatically active formulation, soluble sulfates or phosphates and a dissolved alkali metal silicate and/or ammonium silicate as droplets in a stirred organic, water-immiscible fluid, then converting said silicate to a water-insoluble gel, and thereafter treating the product obtained with a solution of salt of which the cation forms a sparingly soluble sulfate or phosphate.

Claim 13

A process for the preparation of an enzymatically active formulation embedded in silica gel which comprises: suspending an aqueous mixture consisting of an enzymatically active formulation, soluble sulfates or phosphates and a dissolved alkali metal silicate and/or ammonium silicate as droplets in a stirred organic, water-immiscible fluid, then converting said silicate to a water-insoluble gel, and thereafter treating the product obtained with a solution of salt of which the cation forms a sparingly soluble sulfate or phosphate.