US4894137A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme electrode
Assignee: Omron Healthcare Co Ltd
•
Filing: 1987-09-14
•
Granted: 1990-01-16
•
Expires: 2007-09-14
Abstract
Abstract An enzyme electrode comprising: an insulative base plate; two or more electrodes formed on the surface of the insulative base plate and each having an exposed portion; an insulative protection film to insulate and protect the electrodes excluding at least those exposed portions; and an immobilized enzyme film to integratedly cover the exposed portions.
Inventors
- 👤 Koichi Takizawa
- 👤 Satoshi Nakajima
- 👤 Masato Arai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 20:36
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
93 words
An enzyme electrode comprising: an insulative base; at least two electrodes including a working electrode and a reference electrode provided on the surface of said insulative base and each having an exposed portion and a connecting portion, the area of the exposed portion of the reference electrode being larger than that of the exposed portion of the working electrode; an insulative protection film covering the portions excluding said exposed portions and connecting portions of said electrodes; and an immobilized enzyme film which covers integrally at least the exposed portions of said working electrode.
Claim 2
Dependent Claim
93 words
An enzyme electrode comprising: an insulative base; at least two electrodes including a working electrode and a reference electrode provided on the surface of said insulative base and each having an exposed portion and a connecting portion, the area of the exposed portion of the reference electrode being larger than that of the exposed portion of the working electrode; an insulative protection film covering the portions excluding said exposed portions and connecting portions of said electrodes; and an immobilized enzyme film which covers integrally at least the exposed portions of said working electrode.
Claim 3
Dependent Claim
34 words
An enzyme electrode according to claim 1, wherein windows are formed in said insulative protection film and parts of said electrodes are exposed from said windows, thereby forming said exposed portions and connecting portions.
Claim 4
Dependent Claim
18 words
An enzyme electrode according to claim 1, wherein said insulative protection film is made of a photosensitive resin.
Claim 5
Dependent Claim
22 words
An enzyme electrode according to claim 4, wherein the electrodes are respectively formed on at least two surfaces of said insulative base.
Claim 6
Dependent Claim
45 words
An enzyme electrode according to claim 1, wherein said insulative base consists of a flexible insulative base plate, and said electrode comprises conductor patterns formed on the surface of said base plate and one or two or more plating layers laminated on said conductor patterns.
Claim 7
Dependent Claim
66 words
An enzyme electrode according to claim 1, wherein said insulative base consists of a flexible insulative base plate, said electrodes are made of conductive pastes formed on said base plate, another conductor film is laminated on the exposed portion of at least one of said electrodes, and the exposed portion onto which at least said conductor film is laminated is covered by the immobilized enzyme film.
Claim 8
Dependent Claim
72 words
An enzyme electrode according to claim 1, wherein said insulative base consists of a flexible insulative base plate, said electrodes are made of conductive pastes formed on said insulative base plate, a metal foil is adhered onto the exposed portion of at least one of the electrodes by a conductive adhesive agent, and the exposed portion on which at least said metal foil is adhered is covered by the immobilized enzyme film.
Claim 9
Dependent Claim
16 words
An enzyme electrode according to claim 1, wherein said insulative base is a flexible insulative base.
Claim 10
Dependent Claim
35 words
An enzyme electrode according to claim 1, wherein the area of the exposed portion of the reference electrode is at least 20 times as large as that of the exposed portion of the working electrode.
Claim 11
Dependent Claim
30 words
An enzyme electrode according to claim 1, wherein the working electrode, the reference electrode, the insulative protection film and the immobilized enzyme film are integrally fabricated on the insulative base.
Claim 12
Dependent Claim
112 words
A combination enzyme electrode, comprising: a pair of insulative bases; a working electrode provided on the surface of one of said insulative bases and a reference electrode provided on the surface of the other of said insulative bases, each of said working electrode and said reference electrode having an exposed portion and a connecting portion, the area of the exposed portion of said reference electrode being larger than that of the exposed portion of said working electrode; insulative protection films covering the portions of said insulative bases excluding said exposed portions and connecting portions of said electrodes; and an immobilized enzyme film which integrally covers the exposed portion of said working electrode.
Claim 13
Dependent Claim
112 words
A combination enzyme electrode, comprising: a pair of insulative bases; a working electrode provided on the surface of one of said insulative bases and a reference electrode provided on the surface of the other of said insulative bases, each of said working electrode and said reference electrode having an exposed portion and a connecting portion, the area of the exposed portion of said reference electrode being larger than that of the exposed portion of said working electrode; insulative protection films covering the portions of said insulative bases excluding said exposed portions and connecting portions of said electrodes; and an immobilized enzyme film which integrally covers the exposed portion of said working electrode.
Claim 14
Dependent Claim
35 words
An enzyme electrode according to claim 11, wherein the area of the exposed portion of the reference electrode is at least 20 times as large as that of the exposed portion of the working electrode.
Claim 15
Dependent Claim
31 words
An enzyme electrode according to claim 11, wherein the working electrode, the reference electrode, the insulative protection films and the immobilized enzyme film are integrally fabricated on the respective insulative bases.
Claim 16
Dependent Claim
109 words
An enzyme electrode comprising: an insulative base plate; two or more electrodes provided on the surface of said insulative base plate and each having an exposed portion; an insulative protection film insulating and protecting said electrodes except for said exposed portions; and an immobilized enzyme film which integrally covers said exposed portion of at least one of said electrodes, said immobilized enzyme film comprising a first protective film formed on the portion including said exposed portion for preventing said electrode from sensing interfering materials, an enzyme film, and a second protective film for covering said enzyme film, said enzyme film being sandwiched between said first and second protective films.
Claim 17
Dependent Claim
109 words
An enzyme electrode comprising: an insulative base plate; two or more electrodes provided on the surface of said insulative base plate and each having an exposed portion; an insulative protection film insulating and protecting said electrodes except for said exposed portions; and an immobilized enzyme film which integrally covers said exposed portion of at least one of said electrodes, said immobilized enzyme film comprising a first protective film formed on the portion including said exposed portion for preventing said electrode from sensing interfering materials, an enzyme film, and a second protective film for covering said enzyme film, said enzyme film being sandwiched between said first and second protective films.
Claim 18
Dependent Claim
18 words
An enzyme electrode according to claim 14, wherein said first and second protective films are acetyl cellulose films.
Claim 19
Dependent Claim
35 words
An enzyme electrode according to claim 14, wherein the area of the exposed portion of the reference electrode is at least 20 times as large as that of the exposed portion of the working electrode.
Claim 20
Dependent Claim
30 words
An enzyme electrode according to claim 14, wherein the working electrode, the reference electrode, the insulative protection film and the immobilized enzyme film are integrally fabricated on the insulative base.
Claim 21
Dependent Claim
107 words
An enzyme electrode comprising: an insulative base plate; an electrode provided on the surface of said insulative base plate and having an exposed portion; an insulative protection film which insulates and protects said electrodes except for said exposed portion; and an immobilized enzyme film which integrally covers said exposed portion of at least one of said electrodes, said immobilized enzyme film comprising a first protective film formed on the portion including said exposed portion for preventing said electrode from sensing interfering materials, an enzyme film, and a second protective film for covering said enzyme film, said enzyme film being sandwiched between said first and second protective films.
Claim 22
Dependent Claim
107 words
An enzyme electrode comprising: an insulative base plate; an electrode provided on the surface of said insulative base plate and having an exposed portion; an insulative protection film which insulates and protects said electrodes except for said exposed portion; and an immobilized enzyme film which integrally covers said exposed portion of at least one of said electrodes, said immobilized enzyme film comprising a first protective film formed on the portion including said exposed portion for preventing said electrode from sensing interfering materials, an enzyme film, and a second protective film for covering said enzyme film, said enzyme film being sandwiched between said first and second protective films.
Claim 23
Dependent Claim
35 words
An enzyme electrode according to claim 18, wherein the area of the exposed portion of the reference electrode is at least 20 times as large as that of the exposed portion of the working electrode.
Claim 24
Dependent Claim
30 words
An enzyme electrode according to claim 18, wherein the working electrode, the reference electrode, the insulative protection film and the immobilized enzyme film are integrally fabricated on the insulative base.
References Cited (Backward Citations)
US3838034A
Cited
GB1442303A
Cited
US4020830A
Cited
US4020830B1
Cited
US4073713A
Cited
US4218298A
Cited
US4225410A
Cited
JPS56141550A
Cited
US4404066A
Cited
US4356074A
Cited
US4534356A
Cited
US4552840A
Cited
JPS6029658A
Cited
US4655880A
Cited
US4508613A
Cited
US4713165A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-01-15 | 1993-07-17 | 1994-01-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-01-15 | 1997-07-17 | 1998-01-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-01-15 | 2001-07-17 | 2002-01-16 | $7700.00 | Window Closed / Lapsed |