Apparatus and method for sensing species, substances and substrates using oxidase

Claim 2

In combination, a pair of potentiostat operating electrode systems for measuring in an electrolyte the concentration of a substance, species or substrate reactive, in the presence of an enzyme, with an electrochemical constituent of the electrolyte, said electrode systems each including a respective working electrode in association with a counter electrode and a reference electrode, and circuit means for energizing said electrodes while maintaining a substantially constant potential between each working electrode and the associated reference electrode with the reference electrode at equilibrium with the electrolyte, the working electrode of one electrode system interfacing not in the presence of said enzyme with the electrolyte, such that the current flowing between the working and counter electrodes of said one electrode system will be functionally related to balanced but reverse reactions between the electrochemical constituent and the electrolyte at such electrodes, and the working electrode of the other electrode system interfacing in the presence of said enzyme with the electrolyte, such that current flowing between the working and counter electrode of said one system is functionally related to an imbalance between such reverse reactions at the working electrode and counter electrode resulting from consumption of electro-chemical constituent by reaction with the substance, species or substrate.

Claim 3

The combination of claim 1, wherein said electrochemical constituent is oxygen and said enzyme is an oxidase.

Claim 4

The combination of claim 2, further comprising means for locating oxidase with respect to the working electrode of said other electrode system, said oxidase being selected from the group consisting of ______________________________________ E.C. # Enzyme ______________________________________ 1.15.1.1 Superoxide dismustase 1.7.3.1 Nitroethane oxidase 1.4.3.1 D-aspartate oxidase 1.4.3.3 D-amino acid oxidase 1.4.3.2 L-amino acid oxidase 1.4.3.4 Amine oxidase 1.4.3.5 Pyridox amine phosphate oxidase 1.4.3.6 Amine oxidase 1.4.3.7 D-glutamate oxidase 1.4.3.8 Ethanolamine oxidase 1.2.3.3 Pyruvate oxidase 1.2.3.4 Oxalate oxidase 1.1.3.1 Glycollate oxidase 1.1.3.4 Glucose oxidase 1.1.3.5 Hexose oxidase 1.1.3.6 Cholesterol oxidase 1.1.3.7 Aryl alcohol oxidase 1.1.3.8 L-gulonolactone oxidase 1.1.3.9 Golactose oxidase 1.1.3.10 Pyranose oxidase 1.1.3.11 L-Sorbose oxidase 1.1.3.12 Pyridoxine 4-oxidase 1.1.3.13 Alcohol oxidase 1.1.3.15 L-2-hydroxyacid oxidase 1.3.3.1 Dehydro-oratate oxidase 1.3.3.2 Lathosterol oxidase 1.5.3.1 Sarcosine oxidase 1.5.3.2 N--Methylamino acid oxidase 1.5.3.4 N.sup.6 --Methyl lysine oxidase 1.5.3.5 6-hydroxy-L-nicotine oxidase 1.5.3.6 6-hydroxy-D-nicotine oxidase 1.8.3.2 Sulphite oxidase 1.10.3.5 3-hydroxyanthranilate oxidase 1.2.3.1 Aldehyde oxidase 1.2.3.2 Xanthine oxidase ______________________________________

Claim 5

The combination of claim 2, wherein said electrode systems each includes respective working, reference and counter electrodes separated from the electrodes of the system.

Claim 6

The combination of claim 4, further comprising output means for measuring currents flowing between said respective counter electrodes and said respective working electrodes, whereby the difference between the currents is a representation of concentration of the substance, species or substrate in the presence of an oxidase.

Claim 7

The combination of claim 5, said circuit means comprising two circuit portions respectively associated with said one and other electrode systems, one circuit portion comprising means for maintaining a constant voltage between said working electrode and reference electrode of said one electrode system, and the other circuit portion comprising means for maintaining the voltage applied across the working electrode and reference electrode of said other electrode system substantially constant.

Claim 8

The combination of claim 6, said measuring means comprising a differential amplifier for automatically comparing the magnitudes of the currents to produce an output proportionally representative of the concentration of the substance, species or substrate in the presence of an oxidase.

Claim 9

The combination of claim 4, said oxidase comprising an oxidase material contained in a gel immobilized on said working electrode of said other electrode system.

Claim 10

The combination of claim 2, wherein both said electrode systems share a single reference electrode and a single counter electrode.

Claim 11

The combination of claim 9, further comprising electrically nonconductive support means for commonly supporting all of said electrodes.

Claim 12

The combination of claim 9, further comprising output means for measuring currents flowing between said counter electrode and said respective working electrodes, whereby the difference between the currents is a representation of concentration of the substance, species or substrate in the presence of an oxidase.

Claim 13

The combination of claim 11, said measuring means comprising a differential amplifier for automatically comparing the magnitudes of the currents to produce an output proportionally representative of concentration of the substance, species or substrate in the presence of an oxidase.

Claim 14

The combination of claim 9, said oxidase comprising an oxidase material contained in a gel immobilized on said working electrode of said other electrode system.

Claim 15

The combination of claim 1, wherein each of said working electrodes has plural prongs, each of the prongs of a respective working electrode being joined to sum currents therefrom and wherein at least one of said counter and reference electrodes also has prong receiving portions for interdigitated positional relation with respect to the prongs of said working electrodes.

Claim 16

The combination of claim 1, wherein each of said working electrodes is sheet-like and has individual electrode portions and comprises a layer of electrically conductive material on an electrically non-conductive substrate, a layer of electrically insulating material on at least a portion of said layer of electrically insulating material to expose individual plural electrode portions.

Claim 17

The combination of claim 1, said electrodes comprising electrode material deposited on an electrically non-conductive substrate by thick film or thin film technique.

Claim 18

The combination of claim 1, further comprising a substrate of flexible polymeric material, and said electrodes being formed on said substrate by thick film or thin film technique.

Claim 19

The combination of claim 17, said substrate being of a size having orthogonal surface area dimensions on the order of from about one micron to ten milimeters by about one micron to ten milimeters.

Claim 20

Apparatus for measuring the concentration of a substance, species or substrate in an electrolyte, comprising first and second electrode system means for sensing the substance, specie or substrate, each of said electrode system means comprising a respective working electrode and sharing both a reference electrode and a counter electrode with the other electrode system means; support means for supporting said electrodes; circuit means for energizing said electrodes; output means for measuring currents flowing between said shared counter electrode and said respective working electrodes; and an oxidase material on the working electrode of one of said first and second electrode system means, whereby the difference between the currents is a representation of oxygen consumption as a function of concentration of the substances, specie or substrate.

Claim 21

Apparatus for measuring the concentration of a substance, species or substrate in an electrolyte, comprising first and second electrode system means for sensing the substance, specie or substrate, each of said electrode system means comprising a respective working electrode and sharing both a reference electrode and a counter electrode with the other electrode system means; support means for supporting said electrodes; circuit means for energizing said electrodes; output means for measuring currents flowing between said shared counter electrode and said respective working electrodes; and an oxidase material on the working electrode of one of said first and second electrode system means, whereby the difference between the currents is a representation of oxygen consumption as a function of concentration of the substances, specie or substrate.

Claim 22

The apparatus of claim 19, said electrodes comprising electrode material deposited on an electrically non-conductive substrate by thick film or thin film technique.

Claim 23

The apparatus of claim 19, said support means comprising electrically nonconductive support means for commonly supporting all of said electrodes.

Claim 24

The apparatus of claim 19, wherein said oxidase is selected from the group consisting of ______________________________________ E.C. # Enzyme ______________________________________ 1.15.1.1 Superoxide dismustase 1.7.3.1 Nitroethane oxidase 1.4.3.1 D-aspartate oxidase 1.4.3.3 D-amino acid oxidase 1.4.3.2 L-amino acid oxidase 1.4.3.4 Amine oxidase 1.4.3.5 Pyridox amine phosphate oxidase 1.4.3.6 Amine oxidase 1.4.3.7 D-glutamate oxidase 1.4.3.8 Ethanolamine oxidase 1.2.3.3 Pyruvate oxidase 1.2.3.4 Oxalate oxidase 1.1.3.1 Glycollate oxidase 1.1.3.4 Glucose oxidase 1.1.3.5 Hexose oxidase 1.1.3.6 Cholesterol oxidase 1.1.3.7 Aryl alcohol oxidase 1.1.3.8 L-gulonolactone oxidase 1.1.3.9 Golactose oxidase 1.1.3.10 Pyranose oxidase 1.1.3.11 L-Sorbose oxidase 1.1.3.12 Pyridoxine 4-oxidase 1.1.3.13 Alcohol oxidase 1.1.3.15 L-2-hydroxyacid oxidase 1.3.3.1 Dehydro-oratate oxidase 1.3.3.2 Lathosterol oxidase 1.5.3.1 Sarcosine oxidase 1.5.3.2 N--Methylamino acid oxidase 1.5.3.4 N.sup.6 --Methyl lysine oxidase 1.5.3.5 6-hydroxy-L-nicotine oxidase 1.5.3.6 6-hydroxy-D-nicotine oxidase 1.8.3.2 Sulphite oxidase 1.10.3.5 3-hydroxyanthranilate oxidase 1.2.3.1 Aldehyde oxidase 1.2.3.2 Xanthine oxidase ______________________________________

Claim 25

The apparatus of claim 22, said oxidase comprising an oxidase material contained in a gel immobilized on said working electrode of said second electrode system means.