US4883765A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the quantitative determination of lipoprotein components in a body fluid and method for separating lipoproteins from blood
Assignee: IDL INTERNATIONAL DIAGNOSTIC LABORATORIES Ltd SCIENCE BASED INDUSTRIES CAMPUS HAR HOTZVIM SANHEDRIA MURCHEVET
•
Filing: 1987-08-28
•
Granted: 1989-11-28
•
Expires: 2007-08-28
Abstract
Abstract A method, device and kit for the quantitative determination of lipoprotein components, especially cholesterol and triglycerides, in body fluids, especially human serum, plasma or whole blood utilizing the selective adsorption of lipoproteins on particulate silica. This selective adsorption is also useful for the quantitative separation of lipoproteins from samples of whole blood for diagnostic purposes.
Inventors
- 👤 Ilana Tamir
- 👤 Ben-Zion Kidron
- 👤 Lynn Wang
- 👤 Michael Inbar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 11:54
CPC Classifications
B; B01; B01D; B01D15/00; C; C12; C12Q; C12Q1/00; C12Q1/60; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/92; G01N2800/00; G01N2800/04; G01N2800/044; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/106664
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
76 words
A method for the quantitative determination of total cholesterol in a sample of whole blood, which method comprises: (a) contacting said sample with a quantity of particulate silica which is capable of selectively absorbing all lipoproteins in said sample; (b) separating the non-absorbed components of the sample from the silica with said lipoproteins absorbed thereon; (c) after step (b), releasing said lipoproteins absorbed to said silica; and (d) quantitatively determining the total cholesterol in said lipoproteins.
Claim 2
Independent Claim
76 words
A method for the quantitative determination of total cholesterol in a sample of whole blood, which method comprises: (a) contacting said sample with a quantity of particulate silica which is capable of selectively absorbing all lipoproteins in said sample; (b) separating the non-absorbed components of the sample from the silica with said lipoproteins absorbed thereon; (c) after step (b), releasing said lipoproteins absorbed to said silica; and (d) quantitatively determining the total cholesterol in said lipoproteins.
Claim 3
Dependent Claim
25 words
A method according to claim 1, wherein the separation in step (b) comprises the operation of washing the silica with water or an aqueous solution.
Claim 4
Dependent Claim
12 words
A method according to claim 1, wherein the silica is fumed silica.
Claim 5
Dependent Claim
37 words
A method according to claim 1, wherein said silica is employed in an amount sufficient to absorb the entire sample and the separation in step (b) consists of washing the silica with water or an aqueous solution.
Claim 6
Dependent Claim
18 words
A method according to claim 1, wherein the particulate silica is permanently attached to a solid inert carrier.
Claim 7
Dependent Claim
27 words
A method according to claim 5, wherein said solid inert carrier has at least a part of its surface coated with a layer of said particulate silica.
Claim 8
Dependent Claim
17 words
A method according to claim 6, wherein said solid inert carrier is a glass plate or rod.
Claim 9
Independent Claim
136 words
A method for the quantitative determination of total cholesterol in a sample of whole blood, said method comprising: (a) contacting said sample with particulate silica capable of selectively absorbing all lipoproteins in said sample; (b) separating the non-absorbed components of the sample from the absorbed lipoproteins by washing the silica with water or an aqueous solution; (c) incubating the silica having said lipoprotein absorbed thereon with a reagent solution releasing said lipoprotein absorbed on said silica for determining cholesterol in said released lipoprotein by an enzymatic-colorimetric reaction; (d) removing the silica from the liquid incubation mixture; and (e) evaluating the intensity of the color generated in said liquid mixture by said enzymatic-colorimetric reaction and comparing the intensity with one or more standards of said lipoprotein component so as to determine the total cholesterol in said lipoproteins.
Claim 10
Independent Claim
136 words
A method for the quantitative determination of total cholesterol in a sample of whole blood, said method comprising: (a) contacting said sample with particulate silica capable of selectively absorbing all lipoproteins in said sample; (b) separating the non-absorbed components of the sample from the absorbed lipoproteins by washing the silica with water or an aqueous solution; (c) incubating the silica having said lipoprotein absorbed thereon with a reagent solution releasing said lipoprotein absorbed on said silica for determining cholesterol in said released lipoprotein by an enzymatic-colorimetric reaction; (d) removing the silica from the liquid incubation mixture; and (e) evaluating the intensity of the color generated in said liquid mixture by said enzymatic-colorimetric reaction and comparing the intensity with one or more standards of said lipoprotein component so as to determine the total cholesterol in said lipoproteins.
Claim 11
Dependent Claim
34 words
A method according to claim 8, wherein the evaluation of the color intensity in step (e) is carried out by spectrophotometric measurement of the optical density of the liquid mixture at a suitable wavelength.
Claim 12
Dependent Claim
20 words
A method according to claim 9, wherein the value of the optical density is compared with a standard calibration curve.
References Cited (Backward Citations)
US4110077A
Cited
US4167467A
Cited
US4224031A
Cited
US4211531A
Cited
US4399217A
Cited
US4234317A
Cited
US4486531A
Cited
US4503146A
Cited
US4565652A
Cited
US4701418A
Cited
US4748128A
Cited
US4623628A
Cited
US4656261A
Cited
US4743561A
Cited
JPH101764A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-11-27 | 1993-05-29 | 1993-11-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-11-27 | 1997-05-29 | 1997-11-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-11-27 | 2001-05-29 | 2001-11-28 | $7700.00 | Window Closed / Lapsed |