US4503146A
Expired / Lapsed
US
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Method for eliminating turbidity in a biological fluid and reagent therefor
Assignee: Bayer Corp
•
Filing: 1983-04-05
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Granted: 1985-03-05
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Expires: 2003-04-05
Abstract
Abstract A method for eliminating turbidity in a biological fluid by combining said fluid with a surfactant and an enzyme is disclosed as well as a diagnostic reagent formulation for that purpose.
Inventors
- 👤 Shyun-long Yun
- 👤 Luis P. Le/o/ n
- 👤 Syed I. Ahmad
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 19:45
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/60; C12Q1/34; C12Q1/44; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/104165; Y10T436/105831; Y10T436/107497; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
109 words
In an assay for a component in serum or plasma samples comprising mixing sample with reagent and determining the amount of component therein, the improvement comprising a separate pretreatment step which includes adding the enzymes cholesterol esterase, lipase or mixtures thereof and at least one surfactant of the formula: ##STR6## wherein R is alkyl or alkenyl containing from 5 to 17 carbon atoms; and x and y are whole number integers whose sum is no greater than 11 under conditions which permit sample clarification and wherein the amount of said enzyme and said surfactant when combined are sufficient to effectively clear turbidity from the untreated serum or plasma sample.
Claim 2
Dependent Claim
109 words
In an assay for a component in serum or plasma samples comprising mixing sample with reagent and determining the amount of component therein, the improvement comprising a separate pretreatment step which includes adding the enzymes cholesterol esterase, lipase or mixtures thereof and at least one surfactant of the formula: ##STR6## wherein R is alkyl or alkenyl containing from 5 to 17 carbon atoms; and x and y are whole number integers whose sum is no greater than 11 under conditions which permit sample clarification and wherein the amount of said enzyme and said surfactant when combined are sufficient to effectively clear turbidity from the untreated serum or plasma sample.
Claim 3
Dependent Claim
13 words
The assay of claim 1 wherein said sample is a human serum sample.
Claim 4
Dependent Claim
19 words
The assay of claim 1 wherein said conditions comprise incubation at a temperature between room temperature and 45° C.
Claim 5
Dependent Claim
65 words
The assay of claim 1 wherein said surfactant is of the formula wherein x and y are each 1, the enzyme is cholesterol esterase, and the resulting formulation in aqueous buffered form, comprises from about 0.05 g/dl to about 2.5 g/dl of surfactant and at least about 0.025 U/ml of enzyme, said formulation having a pH in the range from about 5.5 to about 7.0.
Claim 6
Dependent Claim
21 words
The assay of claim 4 wherein said surfactant comprises from about 0.1 g/dl to about 0.5 g/dl of the resulting formulation.
Claim 7
Dependent Claim
36 words
The assay of claim 4 wherein the buffer is a maleate used in a concentration of from about 0.05M to about 0.5M and the pH of the resulting formulation is from about 5.0 to about 7.0.
Claim 8
Dependent Claim
12 words
The assay of claim 4 wherein said formulation includes a solubility enhancer.
Claim 9
Dependent Claim
28 words
The assay of claim 7 in which said solubility enhancer is sodium cholate or sodium deoxycholate in an amount to provide about 0.25 g/dl of the total formulation.
Claim 10
Dependent Claim
65 words
The assay of claim 1 wherein said surfactant is of the formula wherein x and y are each 1, the enzyme is a lipase, and the resulting formulation, in aqueous buffered form, comprises from about 0.05 g/dl to about 2.5 g/dl of surfactant and at least about 1.0 U/ml of enzyme, said formulation having a pH in the range from about 5.5 to about 8.0.
Claim 11
Dependent Claim
21 words
The assay of claim 9 wherein said surfactant comprises from about 0.1 g/dl to about 0.5 g/dl of the resulting formulation.
Claim 12
Dependent Claim
36 words
The assay of claim 9 wherein the buffer is a maleate used in a concentration of from about 0.05M to about 0.5M and the pH of the resulting formulation is from about 5.0 to about 7.0.
Claim 13
Dependent Claim
12 words
The assay of claim 9 wherein said formulation includes a solubility enhancer.
Claim 14
Dependent Claim
28 words
The assay of claim 12 in which said solubility enhancer is sodium cholate or sodium deoxycholate in an amount to provide about 0.25 g/dl of the total formulation.
Claim 15
Dependent Claim
24 words
The assay of claim 1 wherein said surfactant is of the formula in which R is alkyl and x and y are each 1.
Claim 16
Dependent Claim
12 words
The assay of claim 14 wherein said surfactant is lauric acid diethanolamide.
Claim 17
Dependent Claim
12 words
The assay of claim 14 wherein said surfactant is myristic acid diethanolamide.
Claim 18
Dependent Claim
12 words
The assay of claim 14 wherein said surfactant is capric acid diethanolamide.
Claim 19
Dependent Claim
24 words
The assay of claim 1 wherein said surfactant is of the formula in which R is alkenyl and x and y are each 1.
Claim 20
Dependent Claim
12 words
The assay of claim 18 wherein said surfactant is oleic acid diethanolamide.
Claim 21
Dependent Claim
12 words
The assay of claim 18 wherein said surfactant is coco acid diethanolamide.
Claim 22
Dependent Claim
16 words
The assay of claim 1 in which said enzyme is derived from animal or microbial source.
Claim 23
Dependent Claim
17 words
The assay of claim 21 in which said enzyme is cholesterol esterase derived from animal pancreatic tissue.
Claim 24
Dependent Claim
65 words
The assay of claim 1 wherein the surfactant is of the formula wherein x+y is no greater than 5, the enzyme is cholesterol esterase, and the resulting formulation, in aqueous buffered form, comprises from about 0.05 g/dl to about 2.5 g/dl of surfactant and at least about 0.025 U/ml of enzyme, said formulation having a pH in the range from about 5.5 to about 8.0.
Claim 25
Dependent Claim
36 words
The assay of claim 23 wherein the buffer is a maleate used in a concentration of from about 0.05M to about 0.5M and the pH of the resulting formulation is from about 5.0 to about 7.0.
Claim 26
Dependent Claim
65 words
The assay of claim 1 wherein the surfactant is of the formula wherein x+y is no greater than 5, the enzyme is a lipase, and the resulting formulation, in aqueous buffered form, comprises from about 0.05 g/dl to about 0.5 g/dl of surfactant and at least about 1.0 U/ml of enzyme, said formulation having a pH in the range from about 2.0 to about 10.0.
Claim 27
Dependent Claim
42 words
The assay of claim 25 wherein the buffer is selected from maleate, citrate, succinate, Tris or borate used in a concentration of from about 0.05M to about 0.5M and the pH of the resulting formulation is from about 2.0 to about 10.0.
Claim 28
Dependent Claim
16 words
The assay of claim 1 in which said enzyme is derived from animal or microbial source.
Claim 29
Dependent Claim
17 words
The assay of claim 27 in which said enzyme is cholesterol esterase derived from animal pancreatic tissue.
Claim 30
Dependent Claim
31 words
The assay of claim 1 wherein the surfactant is of the formula in which R is alkyl, the sum of x and y is 5, and said enzyme is a lipase.
Claim 31
Dependent Claim
14 words
The assay of claim 29 which includes polyethylene glycol p-isooctyl phenyl ether (Triton X-100).
Claim 32
Dependent Claim
53 words
The assay of claim 1 wherein the surfactant comprises a mixture of lauric acid diethanolamide and epoxylated lauric acid (x+y=5) and the resulting formulation, in aqueous buffered form, comprises from about 0.05 g/dl to about 2.0 g/dl of surfactant, said formulation having a pH in the range from about 2.0 to about 10.0.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-03-04 | 1988-09-03 | 1989-03-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-03-04 | 1992-09-03 | 1993-03-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-03-04 | 1996-09-03 | 1997-03-05 | $7700.00 | Window Closed / Lapsed |