Compositions for the detection of high levels of hydrogen peroxide

Claim 2

A dye development composition comprising 1,10-phenanthroline and 1,7-dihydroxynaphthalene.

Claim 3

The composition of claim 1 also comprising a peroxidase active material.

Claim 4

The composition of claim 1 or 2 wherein each chemical component is present in a sufficient concentration to at least react stoichiometrically with an expected concentration of an analyte.

Claim 7

The method of claim 4 wherein the photometric determinations are carried out at wavelengths greater than 620 nanometers.

Claim 8

The method of claim 4 wherein the photometric determination is carried out at about 670 nanometers.

Claim 11

The method as in claim 4 or 7 wherein the aqueous solution is blood serum.

Claim 12

An analytical element for determining hydrogen peroxide levels in aqueous fluids comprising an absorbent material containing a peroxidase active material; 1,10-phenanthroline and 1,7-dihydroxynaphthalene.

Claim 13

An analytical element for determining hydrogen peroxide levels in aqueous fluids comprising an absorbent material containing a peroxidase active material; 1,10-phenanthroline and 1,7-dihydroxynaphthalene.

Claim 14

A multilayer analytical element for determination of hydrogen peroxide in an aqueous medium comprising a support bearing in the following order; (a) one or more reagent layer(s) comprising a peroxidase active substance, 1,10-phenanthroline and 1,7-dihydroxynaphthalene; wherein the peroxidase active substance and 1,10-phenanthroline and 1,7-dihydroxynaphthalene are in the same reagent layers; and (b) a spreading layer in fluid contact with the reagent layer(s).

Claim 15

A multilayer analytical element for determination of hydrogen peroxide in an aqueous medium comprising a support bearing in the following order; (a) one or more reagent layer(s) comprising a peroxidase active substance, 1,10-phenanthroline and 1,7-dihydroxynaphthalene; wherein the peroxidase active substance and 1,10-phenanthroline and 1,7-dihydroxynaphthalene are in the same reagent layers; and (b) a spreading layer in fluid contact with the reagent layer(s).

Claim 16

The element of claim 10 comprising two or more reagent layers wherein one of the reagent layers comprises a material which oxidizes glucose to produce hydrogen peroxide and the second reagent layer comprises 1,10-phenanthroline and 1,7-dihydroxynaphthalene.

Claim 17

The element of claim 10 comprising a combination spreading and reagent layer in contact with a second reagent layer.

Claim 18

The element of claim 12 wherein a) the spreading and reagent layer comprises from 1 to 8 g/m 2 of NaH 2 PO 4 ; from 2 to 5 g/m 2 of polyvinyl pyrrolidone-K90 reagent; from 10,000 to 50,000 I.U. of peroxidase; from 10,000 to 50,000 I.U. of glucose oxidase and from 0.05 to 0.5 g/m 2 of a non ionic surfactant; and (b) the reagent layer comprises from 0.5 to 1.5 g/m 2 1,10-phenanthroline; from 0.5 to 1.5 g/m 2 1,7-dihydroxynaphthalene; from 1 to 5 g/m 2 of polyvinyl pyrrolidone-K90 reagent; from 0.1 to 3 g/m 2 of poly(styrene-co-m+p-N-vinyl-benzyl-N-benzyl-N,N-dimethyl ammonium chloride (60,40)-co-divinylbenzene (49.5; 49.5; 1) and from 0.05to -5 g/m 2 of a non ionic surfactant.

Claim 19

A multilayer analytical element for determination of hydrogen peroxide in an aqueous medium comprising a support bearing in the following order; (a) one or more reagent layer(s) comprising a peroxides active substance, 1, 10-phenanthroline and 1,7-dihydroxynaphthalene; wherein the peroxidase active substance and 1,10-phenanthroline and 1,7-dihydroxynaphthalene are in different reagent layers; and (b) a spreading layer in fluid contact with the reagent layer(s).

Claim 20

A multilayer analytical element for determination of hydrogen peroxide in an aqueous medium comprising a support bearing in the following order; (a) one or more reagent layer(s) comprising a peroxides active substance, 1, 10-phenanthroline and 1,7-dihydroxynaphthalene; wherein the peroxidase active substance and 1,10-phenanthroline and 1,7-dihydroxynaphthalene are in different reagent layers; and (b) a spreading layer in fluid contact with the reagent layer(s).