US4732736A
Expired / Lapsed
US
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Analytical element for the detection hydrogen peroxide
Assignee: Konica Minolta Inc
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Filing: 1985-11-15
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Granted: 1988-03-22
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Expires: 2005-11-15
Abstract
Abstract An analytical element for the detection of hydrogen peroxide comprising a peroxidase-functional material, a hydrogen donor and a coupler, wherein the peroxidase-functional material and hydrogen donor are arranged so as to be separate from each other, for example in separate layers, but are made interactive by the application of a fluid sample.
Inventors
- 👤 Morio Kobayashi
- 👤 Kenichiro Okaniwa
- 👤 Mikio Koyama
- 👤 Kunihiro Furukawa
- 👤 Soichi Zanma
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 15:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; G01N33/526; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/904; Y10T; Y10T436/00; Y10T436/20; Y10T436/206664
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
59 words
In a multilayered analytical element for detecting hydrogen peroxide which comprises peroxidase-functional material, a hydrogen donor and a coupler, the improvement comprising arranging said peroxidase-functional material and said hydrogen donor in different layers in a manner so that they are separated from each other but are capable of interacting when a liquid sample is applied to the analytical element.
Claim 2
Dependent Claim
59 words
In a multilayered analytical element for detecting hydrogen peroxide which comprises peroxidase-functional material, a hydrogen donor and a coupler, the improvement comprising arranging said peroxidase-functional material and said hydrogen donor in different layers in a manner so that they are separated from each other but are capable of interacting when a liquid sample is applied to the analytical element.
Claim 3
Dependent Claim
30 words
The analytical element of claim 1, wherein said hydrogen donor is selected from the group consisting of 4-substituted antipyrine compounds, 2-hydrazonobenzothiazoline compounds, p-halogenophenol compounds, and o- and p-phenylene diamine compounds.
Claim 4
Dependent Claim
28 words
The analytical element of claim 1, wherein said coupler is selected from the group consisting of acylacetoamide compounds, pyrazolone compounds, phenol compounds, naphthol compounds and N,N-di-substituted aniline compounds.
Claim 5
Dependent Claim
23 words
The analytical element of claim 1, wherein said analytical element comprises a support, and wherein one of said layers is a spreading layer.
Claim 6
Dependent Claim
22 words
The analytical element of claim 4, wherein one of said peroxidase-functional material and said hydrogen donor is contained in said spreading layer.
Claim 7
Dependent Claim
26 words
The analytical element of claim 1, wherein the content of said peroxidase-functional material is in the range of 100 U/m 2 to 1,000,000 U/m 2 .
Claim 8
Dependent Claim
26 words
The analytical element of claim 6, wherein the content of said peroxidase-functional material is in the range of 1,000 U/m 2 to 100,000 U/m 2 .
Claim 9
Dependent Claim
28 words
The analytical element of claim 1, wherein the content of said hydrogen donor is in the range of 0.01 m mol/m 2 to 100 m mol/m 2 .
Claim 10
Dependent Claim
28 words
The analytical element of claim 8, wherein the content of said hydrogen donor is in the range of 0.05 m mol/m 2 to 50 m mol/m 2 .
Claim 11
Dependent Claim
27 words
The analytical element of claim 1, wherein the content of said coupler is in the range of 0.1 m mol/m 2 to 100 m mol/m 2 .
Claim 12
Dependent Claim
27 words
The analytical element of claim 10, wherein the content of said coupler is in the range of 0.5 m mol/m 2 to 50 m mol/m 2 .
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-03-22 | 1991-09-21 | 1992-03-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-03-22 | 1995-09-21 | 1996-03-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-03-22 | 1999-09-21 | 2000-03-22 | $7700.00 | Window Closed / Lapsed |