US4870026A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Non-A, non-B. hepatitis, virus, methods of identification purification, characterization, diagnosis and immunization
Assignee: General Hospital Corp
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Filing: 1984-02-08
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Granted: 1989-09-26
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Expires: 2004-02-08
Abstract
Abstract A purified form of a DNA virus which has the following characteristics: molecular weight greater than 2x106 Daltons; substantial immunoreactivity towards an anti-HBsAg monoclonal antibody obtained from cell line ATCC HB 8058; substantially no immunoreactivity towards an anti-HBsAg monoclonal antibody obtained from cell line ATCC CRL 8018; concentration dependent immunoreactivity towards polyclonal IgG anti-HBsAg antibodies, which increases with increased concentration of said DNA virus; discrete particulate form when observed by immunoelectron microscopy in the presence of IgM antibodies from cell line ATCC HB 8058; the DNA of said virus showing hybridization with DNA from hepatitis B viral DNA; and said DNA virus showing, in chimpanzees, infectivity having the characteristics of non A, non B hepatitis.
Inventors
- 👤 Jack Wands
- 👤 David Shafritz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 19:05
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; G01N33/5767; A; A61; A61K; A61K39/00; A61K39/12; A61K39/29; C; C12; C12N; C12N2770/00; C12N2770/00011; C12N2770/24011; C12N2770/24211; C12N2770/24234
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
92 words
A method of diagnosing the NANB disease state in an animal sample which comprises the steps of: (A) confirming the presence of a disease state which is either HBV or NANB in said sample by means of an immunoassay which utilizes a monoclonal antibody which is cross-reactive with HBV viral protein and which is not specific for HBV viral protein; and (B) positively identifying the presence of the NANB disease state by confirming the absence of HBV viral protein by means of an immunoassay utilizing a monoclonal antibody which is HBV specific.
Claim 2
Independent Claim
92 words
A method of diagnosing the NANB disease state in an animal sample which comprises the steps of: (A) confirming the presence of a disease state which is either HBV or NANB in said sample by means of an immunoassay which utilizes a monoclonal antibody which is cross-reactive with HBV viral protein and which is not specific for HBV viral protein; and (B) positively identifying the presence of the NANB disease state by confirming the absence of HBV viral protein by means of an immunoassay utilizing a monoclonal antibody which is HBV specific.
Claim 3
Dependent Claim
20 words
The method of claim 1 wherein said step A immunoassay is performed using a monoclonal IgM antibody or viral protein.
Claim 4
Dependent Claim
13 words
The method of claim 2 wherein said immunoassay method is a sandwich immunoassay.
Claim 5
Dependent Claim
26 words
The method of claim 3 wherein said sandwich immonoassay utilizes a first solid phase bound IgM monoclonal antibody and a second detectably labeled IgM monoclonal antibody.
Claim 6
Dependent Claim
23 words
The method of claim 1 wherein said monoclonal antibody of step B is an IgM antibody obtained from cell line ATCC CRL 8018.
Claim 7
Dependent Claim
23 words
The method of claim 1 wherein said monoclonal antibody of step B is an IgG antibody obtained from cell line ATCC HB 8171.
Claim 8
Dependent Claim
73 words
The method of claim 1 wherein said step (a) comprises a sandwich immunoassay using a first solid phase bound IgM monoclonal antibody obtained from cell line ATCC HB 9801 and a second detectably labeled IgM monoclonal antibody obtained from cell line ATCC HB 9801; and said step (B) comprises an immunoasay utilizing a monoclonal antibody obtained from a cell line selected from the group consisting of ATCC CRL 8018 and ATCC HB 8171.
Claim 9
Dependent Claim
16 words
The method of claim 1 wherein said sample is serum or blood obtained from an animal.
Claim 10
Dependent Claim
11 words
The method of claim 8 wherein said animal is a human.
Claim 11
Dependent Claim
14 words
The method of claim 9 wherein said sample is human blood to be transfused.
Claim 12
Dependent Claim
27 words
The method of claim 2 wherein said IgM antibody is derived from a cell line selected from the group consisting of ATCC HB 9801 or ATCC HB-8170.
Claim 13
Independent Claim
17 words
The method of claim l wherein said IgM antibody is derived from cell line ATCC HB 9801.
Claim 14
Dependent Claim
18 words
The method of claim 4 wherein both said monoclonal antibodies are obtained from cell line ATCC HB 9801.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-09-25 | 1993-03-27 | 1993-09-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-09-25 | 1997-03-27 | 1997-09-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-09-25 | 2001-03-27 | 2001-09-26 | $7700.00 | Window Closed / Lapsed |