US4356164A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Detection of non-A, non-B hepatitis associated antigen
Assignee: United States Department of Health and Human Services
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Filing: 1979-05-21
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Granted: 1982-10-26
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Expires: 1999-05-21
Abstract
Abstract In the detection of the highly transmittable agent of non-A, non-B hepatitis there is described a method utilizing antigen-antibody reaction and a preferred counterelectrophoresis method for the detection of said antigen. The method may be applied as in the recipients of blood transfusions and also may be applied to screening blood donors where the blood donor had transmitted by transfusion non-A, non-B hepatitis antigen several years previously or there was at least a 1-5 year retrospective period from donating blood to retention of active transmittable agent.
Inventors
- 👤 Edward Tabor
- 👤 Robert J. Gerety
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 23:08
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/29; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; G01N33/5767; C; C12; C12N; C12N2770/00; C12N2770/00011; C12N2770/24011; C12N2770/24211; C12N2770/24234; Y; Y10; Y10S; Y10S435/00; Y10S435/966; Y10S436/00; Y10S436/82
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
74 words
A method of assaying for the amount of non-A, non-B hepatitis antigen present in a sample comprising a first step of reacting antigen in the sample with the antibody, a second step of separating reactive phase from non-reactive phase by precipitating antibody from serum and purifying said antibody, and a third step of measuring the extent of the reaction as a measurement of the amount of non-A, non-B hepatitis antigen present in the sample.
Claim 2
Independent Claim
74 words
A method of assaying for the amount of non-A, non-B hepatitis antigen present in a sample comprising a first step of reacting antigen in the sample with the antibody, a second step of separating reactive phase from non-reactive phase by precipitating antibody from serum and purifying said antibody, and a third step of measuring the extent of the reaction as a measurement of the amount of non-A, non-B hepatitis antigen present in the sample.
Claim 3
Dependent Claim
19 words
The method of assaying according to claim 1 wherein the separation of antigen is made by means of counterelectrophoresis.
Claim 4
Dependent Claim
34 words
The method of assaying according to claim 1 wherein the purified antibody is labeled radioactively with a radioactive tracer and the measurement of antigen is made by detecting the radioactivity in the precipitated antibody.
Claim 5
Dependent Claim
15 words
The method of assaying according to claim 3 wherein the radioactive tracer is radioactive iodine.
Claim 6
Dependent Claim
42 words
The method of assaying according to claim 1 wherein the separation of antigen is made by means of agar gel diffusion, which method depends on relative motion of the molecules through the gel where antigen is placed above a column of antibody.
Claim 7
Dependent Claim
39 words
The method of assaying according to claim 1 wherein the separation of antigen is made by means of passive hemagglutination where the presence of a particular antigen is signaled by precipitation or agglutination of erythrocytes which have antigen attached.
Claim 8
Dependent Claim
22 words
The method of assaying according to claim 1 wherein the presence of a particular antibody causes a reaction of antigen in latex.
Claim 9
Dependent Claim
31 words
The method of assaying according to claim 1 wherein the separation of antigen is made where the complement, a lytic substance in serum, is fixed when reacted with an antigen/antibody complex.
Claim 10
Dependent Claim
46 words
In a retrospective period of hepatitis infection preceding transfusion to the donor in the range of 1-5 years, a method of detecting in the serum of a donor the presence of an antigen in non-A, non-B hepatitis by means of an antigen-antibody reaction of claim 1.
References Cited (Backward Citations)
US3867517A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-10-25 | 1986-04-26 | 1986-10-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-10-25 | 1990-04-26 | 1990-10-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-10-25 | 1994-04-26 | 1994-10-26 | $7700.00 | Window Closed / Lapsed |