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US4868106A
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US
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Analytical element and method for determining a component in a test sample
Assignee: Konica Minolta Inc
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Filing: 1986-10-16
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Granted: 1989-09-19
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Expires: 2006-10-16
Abstract
Abstract Disclosed are an analytical element for determining a specific component A in a test sample, based on the specific reaction between said specific component A and a substance B capable of binding specifically to said specific component A, by the use of a labelled material L comprising a label capable of providing a signal and said specific component A or comprising a label capable of providing a signal and a substance C capable of binding specifically to said specific component A, characterized in that said element has a porous reaction layer formed by the use of a mixture containing (a) a carrier having said substance B immobilized thereon and (b) a carrier having an absorbing substance D capable of binding specifically to said labelled material L which has not bound to said substance B or to said specific compound A, to thereby modulate said signal, and an analytical method employing the same.
Inventors
- 👤 Tsukasa Ito
- 👤 Satoshi Kawakatsu
- 👤 Akira Onishi
- 👤 Masayo Ishikawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 14:36
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/968; Y10S436/00; Y10S436/80; Y10S436/807; Y10S436/81; Y10S436/827; Y10S436/828
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
111 words
An analytical element for determining a specific component A in a test sample, based on the specific reaction between said specific component A and a substance B capable of binding specifically to said specific component A, by the use of a labelled material L comprising a label capable of providing a signal and said specific component A, wherein said element has a porous reaction layer forming a mixture of (a) a carrier having immobilized substance B and (b) a carrier having an immobilized absorbing substance D capable of binding specifically to the label of said labelled material L which has not bound to said substance B, to thereby modulate said signal.
Claim 2
Dependent Claim
111 words
An analytical element for determining a specific component A in a test sample, based on the specific reaction between said specific component A and a substance B capable of binding specifically to said specific component A, by the use of a labelled material L comprising a label capable of providing a signal and said specific component A, wherein said element has a porous reaction layer forming a mixture of (a) a carrier having immobilized substance B and (b) a carrier having an immobilized absorbing substance D capable of binding specifically to the label of said labelled material L which has not bound to said substance B, to thereby modulate said signal.
Claim 3
Dependent Claim
31 words
The analytical element according to claim 1, wherein the substance B is selected from the group consisting of an antibody, an antigen, a lectin, a protein A and an enzyme inhibitor.
Claim 4
Dependent Claim
17 words
The analytical element according to claim 2, wherein the substance B is an antibody or an antigen.
Claim 5
Dependent Claim
74 words
The analytical element according to claim 1, wherein combination of said label and said absorbing substance D is selected from the group consisting of an enzyme and an inhibitor for the enzyme; a coenzyme or a prosthetic group and an antibody; an apoenzyme and a prosthetic group; an enzyme precursor and a substance capable of making the enzyme to develop the activity thereof; and a fluorescent substance and an antibody against the fluorescent substance.
Claim 6
Dependent Claim
27 words
The analytical element according to claim 4, wherein the inhibitor in the combination of an enzyme and an inhibitor for the enzyme is an organic mercury compound.
Claim 7
Dependent Claim
14 words
The analytical element according to claim 1, wherein said carrier is particles or fibers.
Claim 8
Dependent Claim
19 words
The analytical element according to claim 6, wherein said particles have a particle size of 1 to 1,000 μm.
Claim 9
Dependent Claim
38 words
The analytical element according to claim 6, wherein said fibers are ones selected from the group consisting of pulp, cotton, hemp, silk, wool, chitin, chitosan, cellulose ester, viscose rayon, cupraammonium rayon, polyamide, polyester, polyolefin, glass fiber and asbestos.
Claim 10
Dependent Claim
17 words
The analytical element according to claim 6, wherein said fibers are ones of 40 to 400 mesh.
Claim 11
Dependent Claim
15 words
Th analytical element according to claim 1, wherein said element additionally contains a regent layer.
Claim 12
Dependent Claim
15 words
The analytical element according to claim 1, wherein said element additionally contains a timing layer.
Claim 13
Dependent Claim
171 words
An analytical method for determining a specific component A in a test fluid sample in a single layer, by employing an analytical element based on the specific reaction between said specific component A and a substance B capable of binding specifically to said specific component A, by the use of a labelled material L comprising a label capable of providing a signal and said specific component A, said element having a porous reaction layer containing a mixture of (a) a carrier having immobilized substance B and (b) a carrier having an immobilized absorbing substance D capable of binding specifically to the label of said labelled material L which has not bound to said substance B, to thereby modulate said signal, the method comprising the steps of: (a) adding said test fluid sample containing said specific component A, and said labelled material L onto said analytical element, and (b) after a predetermined period of reaction time, measuring said modulated signal as a function of said specific component in said test fluid sample.
Claim 14
Dependent Claim
171 words
An analytical method for determining a specific component A in a test fluid sample in a single layer, by employing an analytical element based on the specific reaction between said specific component A and a substance B capable of binding specifically to said specific component A, by the use of a labelled material L comprising a label capable of providing a signal and said specific component A, said element having a porous reaction layer containing a mixture of (a) a carrier having immobilized substance B and (b) a carrier having an immobilized absorbing substance D capable of binding specifically to the label of said labelled material L which has not bound to said substance B, to thereby modulate said signal, the method comprising the steps of: (a) adding said test fluid sample containing said specific component A, and said labelled material L onto said analytical element, and (b) after a predetermined period of reaction time, measuring said modulated signal as a function of said specific component in said test fluid sample.
Claim 15
Dependent Claim
75 words
The analytical method according to claim 12, wherein the method comprises the steps of: (a) mixing a predetermined amount of said test fluid sample containing said specific component A with a predetermined amount of said labelled material L, (b) adding dropwise the thus prepared mixture to said analytical element, and (c) after a predetermined period of reaction time, measuring said modulated signal as a function of said specifical component A in said test fluid sample.
Claim 16
Dependent Claim
118 words
An analytical element for determining a specific component A in a test sample, based on the specific reaction between said specific component A and substance B capable of binding specifically to said specific component A, by the use of a labelled material L comprising a label capable of providing a signal and a substance C capable of binding specifically to said specific component A, wherein said element has a porous reaction layer containing a mixture of (a) a carrier having immobilized B and (b) a carrier having an immobilized absorbing substance D capable of binding specifically to the label of said labelled material L which has not bound to said specific component A, to thereby modulate said signal.
Claim 17
Dependent Claim
118 words
An analytical element for determining a specific component A in a test sample, based on the specific reaction between said specific component A and substance B capable of binding specifically to said specific component A, by the use of a labelled material L comprising a label capable of providing a signal and a substance C capable of binding specifically to said specific component A, wherein said element has a porous reaction layer containing a mixture of (a) a carrier having immobilized B and (b) a carrier having an immobilized absorbing substance D capable of binding specifically to the label of said labelled material L which has not bound to said specific component A, to thereby modulate said signal.
Claim 18
Dependent Claim
31 words
The analytical element according to claim 14, wherein the substance B is selected from the group consisting of an antibody, an antigen, a lectin, a protein A and an enzyme inhibitor.
Claim 19
Dependent Claim
17 words
The analytical element according to claim 15, wherein the substance B is an antibody or an antigen.
Claim 20
Dependent Claim
74 words
The analytical element according to claim 14, wherein combination of said label and said absorbing substance D is selected from the group consisting of an enzyme and an inhibitor for the enzyme; a coenzyme or a prosthetic group and an antibody; an apoenzyme and a prosthetic group; an enzyme precursor and a substance capable of making the enzyme to develop the activity thereof; and a fluorescent substance and an antibody against the fluorescent substance.
Claim 21
Dependent Claim
27 words
The analytical element according to claim 17, wherein the inhibitor in the combination of an enzyme and an inhibitor for the enzyme is an organic mercury compound.
Claim 22
Dependent Claim
14 words
The analytical element according to claim 14, wherein said carrier is particles or fibers.
Claim 23
Dependent Claim
19 words
The analytical element according to claim 19, wherein said particles have a particle size of 1 to 1,000 μm.
Claim 24
Dependent Claim
38 words
The analytical element according to claim 20, wherein said fibers are ones selected from the group consisting of pulp, cotton, hemp, silk, wool, chitin, chitosan, cellulose ester, viscose rayon, cupraammonium rayon, polyamide, polyester, polyolefin, glass fiber and asbestos.
Claim 25
Dependent Claim
17 words
The analytical element according to claim 19, wherein said fibers are ones of 40 to 400 mesh.
Claim 26
Dependent Claim
15 words
The analytical element according to claim 14, wherein said element additionally contains a reagent layer.
Claim 27
Dependent Claim
15 words
The analytical element according to claim 14, wherein said element additionally contains a timing layer.
Claim 28
Dependent Claim
180 words
An analytical method for determining a specific component A in a test fluid sample in a single layer, by employing an analytical element based on the specific reaction between said specific component A and a substance B capable of binding specifically to said specific component A, by the use of a labelled material L comprising a label capable of providing a signal and a substance C capable of binding specifically to said specific component A, said element having a porous reaction layer containing a mixture of (a) a carrier having immobilized substance B and (b) a carrier having an immobilized absorbing substance D capable of binding specifically to the label of said labelled material L which has not bound to said specific component A, to thereby modulate said signal, the method comprising the steps of: (a) adding said test fluid sample containing said specific component A, and said labelled material L onto said analytical element, and (b) after a predetermined period of reaction time, measuring said modulated signal as a function of said specific component in said test fluid sample.
Claim 29
Dependent Claim
180 words
An analytical method for determining a specific component A in a test fluid sample in a single layer, by employing an analytical element based on the specific reaction between said specific component A and a substance B capable of binding specifically to said specific component A, by the use of a labelled material L comprising a label capable of providing a signal and a substance C capable of binding specifically to said specific component A, said element having a porous reaction layer containing a mixture of (a) a carrier having immobilized substance B and (b) a carrier having an immobilized absorbing substance D capable of binding specifically to the label of said labelled material L which has not bound to said specific component A, to thereby modulate said signal, the method comprising the steps of: (a) adding said test fluid sample containing said specific component A, and said labelled material L onto said analytical element, and (b) after a predetermined period of reaction time, measuring said modulated signal as a function of said specific component in said test fluid sample.
Claim 30
Dependent Claim
79 words
The analytical method according to claim 25, wherein the method comprises the steps: (a) adding a predetermined amount of said test fluid sample containing said specific component A to said analytical element, (b) then, after a predetermined period of reaction time, adding a predetermined amount of said labelled material to said analytical element, and (c) after a predetermined period of reaction time, measuring said modulated signal as a function of said specific component A in said test fluid sample.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-09-18 | 1993-03-20 | 1993-09-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-09-18 | 1997-03-20 | 1997-09-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-09-18 | 2001-03-20 | 2001-09-19 | $7700.00 | Window Closed / Lapsed |