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US4859302A
Expired / Lapsed
US
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Electroelution of macromolecules
Assignee: Harvard University
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Filing: 1987-10-22
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Granted: 1989-08-22
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Expires: 2007-10-22
Abstract
Abstract Macromolecules are electroeluted from a matrix by placing the matrix containing the macromolecules of interest in a container, forcing the matrix toward the tip of the container, e.g., by centrifugation, providing an opening at the tip of the container large enough to pass the component, but not the matrix, inserting that container inside another container, placing an electrode in each container together with enough electrophoresis buffer to immerse each electrode, and applying a voltage across the electrodes for a time and under conditions sufficient to cause the macromolecules but not the matrix to migrate from the first container into the second. A kit for performing the electroelution is also described.
Inventors
- 👤 Mark R. Alfenito
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 06:51
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/4473
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
136 words
A method for the electroelution of a macromolecular component from a matrix comprising the steps of: placing a sample comprising said macromolecular component contained within said matrix in a first container; forcing said sample toward the tip of said first container; providing an opening at the first container tip large enough to pass said macromolecular component but not said matrix; inserting said first container into a second container; placing one electrode in said first container and placing a second electrode in said second container, together with enough electrolysis buffer to immerse each said electrode; and applying a voltage across said electrodes for a time and under conditions sufficient to cause said macromolecular component to migrate through the opening in said first container tip and into said second container; thereby separating said macromolecular component from said matrix.
Claim 2
Independent Claim
136 words
A method for the electroelution of a macromolecular component from a matrix comprising the steps of: placing a sample comprising said macromolecular component contained within said matrix in a first container; forcing said sample toward the tip of said first container; providing an opening at the first container tip large enough to pass said macromolecular component but not said matrix; inserting said first container into a second container; placing one electrode in said first container and placing a second electrode in said second container, together with enough electrolysis buffer to immerse each said electrode; and applying a voltage across said electrodes for a time and under conditions sufficient to cause said macromolecular component to migrate through the opening in said first container tip and into said second container; thereby separating said macromolecular component from said matrix.
Claim 3
Dependent Claim
44 words
The method of claim 1 comprising providing a filter in the bottom of said first container, and applying said voltage across said electrodes to cause said macromolecular component to migrate through said filter, said filter preventing migration of said matrix into said second container.
Claim 4
Dependent Claim
27 words
The method of claim 1 wherein said opening at said first container tip is provided after inserting said sample and forcing it toward said first container tip.
Claim 5
Dependent Claim
26 words
The method of claim 1 wherein the tip of at least said first container is tapered, said method comprising forcing said sample into said tapered tip.
Claim 6
Dependent Claim
19 words
The method of claim 1 comprising centrifuging said sample to force it toward the tip of said first container.
Claim 7
Dependent Claim
26 words
The method of claim 1 wherein said second electrode is annular and said method comprises positioning said annular electrode around the outside of said first container.
Claim 8
Dependent Claim
40 words
The method of claim 1 comprising nesting said second container around said first container, said containers being sized and configured to define a volume between them sufficient to immerse said second electrode, said volume being less than about 250 microliters.
Claim 9
Dependent Claim
77 words
The method of claim 1 wherein said second container is supplied with a lid and said method further comprises recovering said macromolecular component directly from said second container by adding to said second container a precipitation agent to cause precipitation of said macromolecular component, closing the lid of said second container, agitating the liquid in said second container until precipitation occurs, and centrifuging the contents of said second container to collect a precipitate of said macromolecular component.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-08-21 | 1993-02-20 | 1993-08-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-08-21 | 1997-02-20 | 1997-08-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-08-21 | 2001-02-20 | 2001-08-22 | $7700.00 | Window Closed / Lapsed |