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US4855045A
Expired / Lapsed
US
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Method and apparatus for the separation of organic substances from a suspension or solution
Assignee: Individual
•
Filing: 1983-01-13
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Granted: 1989-08-08
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Expires: 2003-01-13
Abstract
Abstract A method and apparatus for the separation of cells, organelles or proteins from a suspension or solution thereof, including: (a) coating ferromagnetic beads with an adsorption layer comprising antibodies, haptenes or chemical substrate adapted for binding the cells, organelles or proteins to be separated; (b) introducing said beads to a separation chamber having an entrance port and an exit port, the ratio of total volume of said beads to the chamber volume being of between 0.01 and 0.5, said chamber having magnetic field gradients in the regions of said entrance and exit ports to prevent the beads from leaving the chamber through the entrance or exit ports; and (c) passing the suspension or solution containing said cells, organelles or proteins into the entrance port through the chamber and out of the exit port to effect adsorption of the cells, organelles or proteins on said adsorption layer of the beads.
Inventors
- 👤 Thomas A. Reed
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 18:35
CPC Classifications
B; B03; B03C; B03C1/00; B03C1/005; B03C1/01; B03C1/02; B03C1/025; B03C1/031; B03C1/032; B03C1/033; B03C1/0335; B03C1/28; B03C1/288; C; C12; C12M; C12M47/00; C12M47/02; C12N; C12N1/00; C12N1/02; B03C2201/00; B03C2201/18; B03C2201/26
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
106 words
An apparatus for the separation of cells, organelles, or proteins from a suspension or solution thereof, comprising: (a) a reaction vessel with an inlet and outlet for passing the suspension or solution therethrough; (b) a plurality of magnetic attachment bodies inside the reaction vessel which bodies are provided with an adsorption layer which is specifically active on test substances or with a cell culture layer; and (c) a wire ferromagnetic carrier body within a reaction volume and through which a magnetic field passes; and (d) a magnet arrangement for producing magnetic field gradients which keeps the attachment bodies in the reaction volume inside the reaction vessel.
Claim 2
Independent Claim
106 words
An apparatus for the separation of cells, organelles, or proteins from a suspension or solution thereof, comprising: (a) a reaction vessel with an inlet and outlet for passing the suspension or solution therethrough; (b) a plurality of magnetic attachment bodies inside the reaction vessel which bodies are provided with an adsorption layer which is specifically active on test substances or with a cell culture layer; and (c) a wire ferromagnetic carrier body within a reaction volume and through which a magnetic field passes; and (d) a magnet arrangement for producing magnetic field gradients which keeps the attachment bodies in the reaction volume inside the reaction vessel.
Claim 3
Dependent Claim
23 words
An apparatus as claimed in claim 1 wherein the attachment bodies are spherical and have a diameter of between 10 and 200 μm.
Claim 4
Dependent Claim
31 words
An apparatus as claimed in claim 1 wherein the magnetic field is substantially homogeneous and extends transversely to the flow direction of the suspension or solution passing through the reaction vessel.
Claim 5
Dependent Claim
20 words
An apparatus as claimed in claim 3 wherein the intensity of the magnetic field is between 0.005 and 0.5 Tesla.
Claim 6
Dependent Claim
29 words
An apparatus as claimed in claim 4 wherein the carrier body is formed from wire, the wire diameter being in the range of the diameter of the attachment bodies.
Claim 7
Dependent Claim
18 words
An apparatus as claimed in claim 5 wherein the construction of the carrier body is grid- or sieve-like.
Claim 8
Dependent Claim
17 words
An apparatus as claimed in claim 5 wherein the carrier body is formed from aribitrarily bent wire.
Claim 9
Dependent Claim
23 words
An apparatus as claimed in claim 7 wherein the mean distance between adjacent wire pieces is 5 to 200 times the wire diameter.
Claim 10
Dependent Claim
20 words
An apparatus as claimed in claim 8 wherein the reaction vessel is inserted between the pole shoes of a magnet.
Claim 11
Dependent Claim
38 words
An apparatus as claimed in claim 9 wherein a magnetic field gradient, is produced by a magnet arrangement at least at the outflow end of the reaction volume at which the suspension or solution leaves the reaction volume.
Claim 12
Dependent Claim
24 words
An apparatus as claimed in claim 10 wherein the reaction vessel is cylindrical and has been inserted into the hole of a cylindrical coil.
Claim 13
Dependent Claim
34 words
An apparatus as claimed in claim 10 having an introduction opening, which serves for ventilation, in the reaction vessel for a hollow needle or a hose for feeding attachment bodies into the reaction volume.
Claim 14
Dependent Claim
26 words
An apparatus as claimed in claim 12 having a collection space, which is provided with a discharge valve, for the attachment bodies beneath the reaction volume.
Claim 15
Independent Claim
124 words
An apparatus for the separation of cells, organelles, or proteins from a suspension or solution thereof, comprising: (a) a reaction vessel with an inlet and outlet for passing the suspension or solution therethrough; (b) a plurality of magnetic attachment bodies inside the reaction vessel which bodies are provided with an adsorption layer which is specifically active on test substances or with a cell culture layer; (c) having a stirring bar which is arranged concentrically with the reaction vessel and which moves the attachment bodies in the direction opposite to the flow direction of the suspension or solution passing through the vessel; and (d) a magnet arrangement for producing magnetic field gradients which keeps the attachment bodies in a reaction volume inside the reaction vessel.
Claim 16
Independent Claim
124 words
An apparatus for the separation of cells, organelles, or proteins from a suspension or solution thereof, comprising: (a) a reaction vessel with an inlet and outlet for passing the suspension or solution therethrough; (b) a plurality of magnetic attachment bodies inside the reaction vessel which bodies are provided with an adsorption layer which is specifically active on test substances or with a cell culture layer; (c) having a stirring bar which is arranged concentrically with the reaction vessel and which moves the attachment bodies in the direction opposite to the flow direction of the suspension or solution passing through the vessel; and (d) a magnet arrangement for producing magnetic field gradients which keeps the attachment bodies in a reaction volume inside the reaction vessel.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-08-07 | 1993-02-06 | 1993-08-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-08-07 | 1997-02-06 | 1997-08-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-08-07 | 2001-02-06 | 2001-08-08 | $7700.00 | Window Closed / Lapsed |