US4780113A — Isomobility focusing in a magnetically stabilized…

Claim 1

Independent Claim 172 words

The process of isomobility focusing of a component of a feedstream in a magnetically stabilized fluidized bed which process comprises: a. providing in a column a bed of magnetizable adsorbent solids for which the component has an affinity, said bed descending countercurrently to an ascending flow through the bed of a fluidizing medium which enters the column at a fluidizing medium entry point, and said bed being stabilized by a magnetic means of sufficient strength to suppress solids backmixing and to preserve staging therein; b. introducing the feedstream into the column through at least one feedpoint which is spaced above the fluidizing medium entry point, the feedstream and the fluidizing medium together comprising a fluid phase within the column; and c. adjusting conditions such that they are effective to result in concentration of the component within at least one isomobility focusing zone in the column wherein within said zone an equilibrium is maintained between the velocity of the component in the fluid phase and the velocity of the component on the solids.

Claim 2

Dependent Claim 172 words

The process of isomobility focusing of a component of a feedstream in a magnetically stabilized fluidized bed which process comprises: a. providing in a column a bed of magnetizable adsorbent solids for which the component has an affinity, said bed descending countercurrently to an ascending flow through the bed of a fluidizing medium which enters the column at a fluidizing medium entry point, and said bed being stabilized by a magnetic means of sufficient strength to suppress solids backmixing and to preserve staging therein; b. introducing the feedstream into the column through at least one feedpoint which is spaced above the fluidizing medium entry point, the feedstream and the fluidizing medium together comprising a fluid phase within the column; and c. adjusting conditions such that they are effective to result in concentration of the component within at least one isomobility focusing zone in the column wherein within said zone an equilibrium is maintained between the velocity of the component in the fluid phase and the velocity of the component on the solids.

Claim 3

Dependent Claim 211 words

An isomobility focusing process for separating at least one component of a multi-component feedstream which process comprises: a. providing in a column a bed of magnetizable adsorbent solids for which the at least one component has an affinity, said bed descending countercurrently to an ascending flow through the bed of a fluidizing medium which enters the column at a fluidizing medium entry point, and said bed being stabilized by a magnetic means of sufficient strength to suppress solids backmixing and to preserve staging therein; b. introducing the feedstream into the column through at least one feedpoint which is spaced above the fluidizing medium entry point, the feedstream and the fluidizing medium together comprising a fluid phase within the column: c. adjusting conditions such that they are effective to result in concentration of the at least one component within at least one isomobility focusing zone in the column, wherein within said zone an equilibrium is maintained between the velocity of the component in the fluid phase and the velocity of the component on the solids, the other components of the feedstream being passed from the column; d. discontinuing the introduction of the feedstream; e. terminating the flow of the fluidizing medium; and f. recovering the at least one component from the column.

Claim 4

Dependent Claim 211 words

An isomobility focusing process for separating at least one component of a multi-component feedstream which process comprises: a. providing in a column a bed of magnetizable adsorbent solids for which the at least one component has an affinity, said bed descending countercurrently to an ascending flow through the bed of a fluidizing medium which enters the column at a fluidizing medium entry point, and said bed being stabilized by a magnetic means of sufficient strength to suppress solids backmixing and to preserve staging therein; b. introducing the feedstream into the column through at least one feedpoint which is spaced above the fluidizing medium entry point, the feedstream and the fluidizing medium together comprising a fluid phase within the column: c. adjusting conditions such that they are effective to result in concentration of the at least one component within at least one isomobility focusing zone in the column, wherein within said zone an equilibrium is maintained between the velocity of the component in the fluid phase and the velocity of the component on the solids, the other components of the feedstream being passed from the column; d. discontinuing the introduction of the feedstream; e. terminating the flow of the fluidizing medium; and f. recovering the at least one component from the column.

Claim 5

Dependent Claim 211 words

An isomobility focusing process for separating at least one component of a multi-component feedstream which process comprises: a. providing in a column a bed of magentizable absorbent solids for which the at least one component has an affinity, said bed descending countercurrently to an ascending flow through the bed of a fluidizing medium which enters the column at a fluidizing medium entry point, and said bed being stabilized by a magnetic means of sufficient strength to suppress solids backmixing and to preserve staging therein; b. introducing the feedstream into the column through at least one feedpoint which is spaced above the fluidizing medium entry point, the feedstream and the fluidizing medium together comprising a fluid phase within the column; c. adjusting conditions such that they are effective to result in concentration of the at least one component within at least one isomobility focusing zone in the column, wherein within said zone an equilibrium is maintained between the velocity of the component in the fluid phase and the velocity of the component on the solids, the other components of the feedstream being passed from the column; d. discontinuing the introduction of the feedstream; e. maintaining the flow of the fluidizing medium; and f. recovering the at least one component from the column.

Claim 6

Dependent Claim 211 words

An isomobility focusing process for separating at least one component of a multi-component feedstream which process comprises: a. providing in a column a bed of magentizable absorbent solids for which the at least one component has an affinity, said bed descending countercurrently to an ascending flow through the bed of a fluidizing medium which enters the column at a fluidizing medium entry point, and said bed being stabilized by a magnetic means of sufficient strength to suppress solids backmixing and to preserve staging therein; b. introducing the feedstream into the column through at least one feedpoint which is spaced above the fluidizing medium entry point, the feedstream and the fluidizing medium together comprising a fluid phase within the column; c. adjusting conditions such that they are effective to result in concentration of the at least one component within at least one isomobility focusing zone in the column, wherein within said zone an equilibrium is maintained between the velocity of the component in the fluid phase and the velocity of the component on the solids, the other components of the feedstream being passed from the column; d. discontinuing the introduction of the feedstream; e. maintaining the flow of the fluidizing medium; and f. recovering the at least one component from the column.

Claim 7

Dependent Claim 17 words

Process of claim 3 wherein the at least one component is recovered by elution from the column.

Claim 8

Dependent Claim 15 words

Process of claim 4 wherein the component is recovered from the column by step-wise elution.

Claim 9

Dependent Claim 15 words

Process of claim 4 wherein the component is recovered from the column by gradient elution.

Claim 10

Dependent Claim 207 words

An isomobility focusing process for separating at least one component of a multi-component feedstream which process comprises: a. providing in a column a bed of magnetizable absorbent solids for which the at least one component of the feedstream has an affinity, said bed descending countercurrently to an ascending flow through the bed of a fluidizing medium which enters the column at a fluidizing medium entry point, and said bed being stabilized by a magnetic means of sufficient strength to suppress solids backmixing and to preserve staging therein; b. introducing the feedstream into the column through at least one feedpoint which is spaced above the fluidizing medium entry point, the feedstream and the fluidizing medium together comprising a fluid phase within the column; c. adjusting conditions such that they are effective to result in concentration of the at least one component within at least one isomobility focusing zone in the column, wherein within said zone an equilibrium is maintained between the velocity of the component in the fluid phase and the velocity of the component on the solids, the other components of the feedstream being passed from the column with the fluid or the solids; and d. recovering the at least one component from the isomobility focusing zone.

Claim 11

Dependent Claim 207 words

An isomobility focusing process for separating at least one component of a multi-component feedstream which process comprises: a. providing in a column a bed of magnetizable absorbent solids for which the at least one component of the feedstream has an affinity, said bed descending countercurrently to an ascending flow through the bed of a fluidizing medium which enters the column at a fluidizing medium entry point, and said bed being stabilized by a magnetic means of sufficient strength to suppress solids backmixing and to preserve staging therein; b. introducing the feedstream into the column through at least one feedpoint which is spaced above the fluidizing medium entry point, the feedstream and the fluidizing medium together comprising a fluid phase within the column; c. adjusting conditions such that they are effective to result in concentration of the at least one component within at least one isomobility focusing zone in the column, wherein within said zone an equilibrium is maintained between the velocity of the component in the fluid phase and the velocity of the component on the solids, the other components of the feedstream being passed from the column with the fluid or the solids; and d. recovering the at least one component from the isomobility focusing zone.

Claim 12

Dependent Claim 15 words

Process of claim 7 wherein the at least one component is recovered as a sidestream.

Claim 13

Dependent Claim 10 words

Process of claim 8 wherein the component is recovered continuously.

Claim 14

Dependent Claim 10 words

Process of claim 8 wherein the component is recovered semi-continuously.

Claim 15

Dependent Claim 20 words

Process of claim 1, 2, 3 or 7 wherein conditions are adjusted to provide two isomobility zones within the column.

Claim 16

Dependent Claim 25 words

Process of claim 1, 2, 3 or 7 wherein other components of the feedstream pass from the column with the fluid phase or the solids.

Claim 17

Dependent Claim 22 words

Process of claim 1, 2, 3 or 7 wherein the condition of temperature in the column is adjusted to provide isomobility focusing.

Claim 18

Dependent Claim 22 words

Process of claim 1, 2, 3 or 7 wherein a salt concentration of the fluid phase is adjusted to provide isomobility focusing.

Claim 19

Dependent Claim 25 words

Process of claim 1, 2, 3 or 7 wherein rate of flow of the fluid phase in the column is adjusted to provide isomobility focusing.

Claim 20

Dependent Claim 20 words

The process of claim 1, 2, 3 or 7 wherein the condition of pH is adjusted to provide isomobility focusing.

Claim 21

Dependent Claim 27 words

The process of claim 1, 2, 3 or 7 wherein said magnetizable adsorbent solids comprise adsorbent particles admixed with ferromagnetic particles, ferrimagnetic particles, or a mixture thereof.

Claim 22

Dependent Claim 57 words

The process of claim 1, 2, 3 or 7 wherein said magnetizable adsorbent solids comprise an adsorbent selected from activated aluminas, silica and silica gels, molecular sieves of carbon or zeolite, revers phase chromatography media, exclusion chromatography media, ion-exchange chromatography media, gel ion-exchange media, affinity chromatography media, and adsorbent celluloses or alginates of natural or man-made origins.

Claim 23

Dependent Claim 13 words

The process of claim 18 wherein the adsorbent is an ion exchange resin.

Claim 24

Dependent Claim 18 words

The process of claim 1, 2, 3 or 7 wherein the component of the feedstream is a biomolecule.

Claim 25

Dependent Claim 20 words

The process of claim 1, 2, 3 or 7 wherein the fluidizing medium is a liquid, gas or mixtures thereof.

Claim 26

Dependent Claim 13 words

The process of claim 21 wherein the fluidizing medium is a buffer solution.

Claim 27

Dependent Claim 19 words

The process of claim 1, 2, 3 or 7 wherein the magnetic means is an externally applied magnetic field.

Claim 28

Dependent Claim 26 words

The process of claim 23 wherein said magnetic means is a uniform magnetic field applied externally in a direction co-linear with flow of the fluidizing medium.

Claim 29

Dependent Claim 21 words

The process of claim 1, 2, 3 or 7 wherein the magnetizable adsorbent solids flow in substantially plug-flow through the column.

Claim 30

Dependent Claim 22 words

The process of claim 1, 2, 3 or 7 wherein the magnetizable adsorbent solids recirculate within a closed loop within said column.

Claim 31

Dependent Claim 36 words

The process of claim 1, 2, 3 or 7 wherein the magnetizable absorbent solids are removed from the column and passed to a vessel and said solids are then recirculated from said vessel to the column.

Claim 32

Dependent Claim 28 words

The process of claim 27 wherein said solids in said vessel form a bed which descends countercurrently to an ascending flow through the bed of a fluidizing medium.

Claim 33

Dependent Claim 17 words

The process of claim 28 wherein the bed in the vessel is stabilized by a magnetic means.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1991-10-25	1992-04-25	1992-10-25	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1995-10-25	1996-04-25	1996-10-25	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	1999-10-25	2000-04-25	2000-10-25	$7700.00	Window Closed / Lapsed

Isomobility focusing in a magnetically stabilized fluidized bed

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