Patent US4837167A successfully refreshed from Google Patents.
US4837167A
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Immunoassay for multi-determinant antigens using high-affinity
Assignee: General Hospital Corp
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Filing: 1984-04-24
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Granted: 1989-06-06
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Expires: 2004-04-24
Abstract
Abstract A simultaneous sandwich immunoassay employing high-affinity monoclonal antibodies is disclosed. This simultaneous sandwich assembly has surprising sensitivity compared to forward and reverse sandwich assays for the detection of multi-determinant antigens such as hepatitis B surface antigen.
Inventors
- 👤 Hubert J. P. Schoemaker
- 👤 Jack R. Wands
- 👤 Barbara L. Westrick
- 👤 Vincent R. Zurawski, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 09:40
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/577; G01N33/576; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/819; Y10S436/82; Y10S530/00; Y10S530/806; Y10S530/863; Y10S530/864
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
86 words
In an immunoassay for detecting the presence of a multi-determinant antigen in a liquid sample wherein said sample is simultaneously incubated in the presence of an immunoadsorbent containing immobilized antibody for said antigen and labeled antibody for said antigen, and thereafter detecting the amount of labeled antibody bound to said immunoadsorbent as an indication of the amount of antigen present in said sample: the improvement wherein said immobilized antibody and said labeled antibody comprise high-affinity IgM monoclonal antibodies directed against a multiply-appearing determinant of said antigen.
Claim 2
Dependent Claim
86 words
In an immunoassay for detecting the presence of a multi-determinant antigen in a liquid sample wherein said sample is simultaneously incubated in the presence of an immunoadsorbent containing immobilized antibody for said antigen and labeled antibody for said antigen, and thereafter detecting the amount of labeled antibody bound to said immunoadsorbent as an indication of the amount of antigen present in said sample: the improvement wherein said immobilized antibody and said labeled antibody comprise high-affinity IgM monoclonal antibodies directed against a multiply-appearing determinant of said antigen.
Claim 3
Dependent Claim
20 words
The improvement of claim 1 wherein said labeled monoclonal antibody and said immobilized monoclonal antibody comprise the same monoclonal antibody.
Claim 4
Dependent Claim
21 words
The improvement of claims 1 or 2 wherein said antigen is a viral antigen comprising a virus or antigenically-active portion thereof.
Claim 5
Dependent Claim
14 words
The improvement of claim 3 wherein said viral antigen comprises an hepatitis viral antigen.
Claim 6
Dependent Claim
12 words
The improvement of claim 4 wherein said hepatitis viral antigen comprises HBsAg.
Claim 7
Dependent Claim
127 words
An immunoassay for detecting the presence of a multi-determinant antigen in a liquid sample comprising: a. forming an incubation mixture containing: i. liquid sample: ii. a solid-phase immunoadsorbent containing immobilized high-affinity IgM monoclonal antibody directed against a multiply-appearing determinant of said antigen; and, iii. labeled, soluble, high-affinity, IgM monoclonal antibody directed against a multiply-appearing determinant of said antigen; b. incubating said incubation mixture under conditions and for a period of time sufficient for antigen in the liquid sample to bind to both immobilized monoclonal antibody and labeled, soluble, monoclonal antibody; c. separating said solid-phase immunoadsorbent from the incubation mixture after said incubation; and, d. detecting for labeled monoclonal antibody bound to said solid-phase immunoadsorbent as an indication of the amount of said antigen in the liquid sample.
Claim 8
Dependent Claim
127 words
An immunoassay for detecting the presence of a multi-determinant antigen in a liquid sample comprising: a. forming an incubation mixture containing: i. liquid sample: ii. a solid-phase immunoadsorbent containing immobilized high-affinity IgM monoclonal antibody directed against a multiply-appearing determinant of said antigen; and, iii. labeled, soluble, high-affinity, IgM monoclonal antibody directed against a multiply-appearing determinant of said antigen; b. incubating said incubation mixture under conditions and for a period of time sufficient for antigen in the liquid sample to bind to both immobilized monoclonal antibody and labeled, soluble, monoclonal antibody; c. separating said solid-phase immunoadsorbent from the incubation mixture after said incubation; and, d. detecting for labeled monoclonal antibody bound to said solid-phase immunoadsorbent as an indication of the amount of said antigen in the liquid sample.
Claim 9
Dependent Claim
21 words
An immunoassay of claim 6 wherein said labeled, soluble monoclonal antibody and said immobilized monoclonal antibody comprise the same monoclonal antibody.
Claim 10
Dependent Claim
22 words
An immunoassay of claims 6 or 7 wherein said multi-determinant antigen is a viral antigen comprising a virus or antigenically-active portion thereof.
Claim 11
Dependent Claim
14 words
An immunoassay of claim 8 wherein said viral antigen comprises an hepatitis viral antigen.
Claim 12
Dependent Claim
12 words
An immunoassay of claim 9 wherein said hepatitis viral antigen comprises HBsAg.
Claim 13
Dependent Claim
15 words
An immunoassay of claim 10 wherein said labeled, soluble, monoclonal antibody contains a radioactive label.
Claim 14
Dependent Claim
12 words
An immunoassay of claim 11 wherein said radioactive label comprises 125 I.
Claim 15
Dependent Claim
17 words
An immunoassay of claim 10 wherein said labeled, soluble, monoclonal antibody is labeled with an enzyme label.
Claim 16
Dependent Claim
17 words
An immunoassay of claim 10 wherein said labeled, soluble, monoclonal antibody is labeled with an optical label.
Claim 17
Dependent Claim
13 words
An immunoassay of claim 14 wherein said optical label comprises a fluorescent material.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1992-06-05 | 1992-12-05 | 1993-06-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-06-05 | 1996-12-05 | 1997-06-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-06-05 | 2000-12-05 | 2001-06-06 | $7700.00 | Window Closed / Lapsed |