EP0045103A2
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EP
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Method for the determination of antigens with the aid of two or more monoclonal antibodies
Assignee: Akzo Nobel NV
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Filing: 1981-07-06
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Granted: —
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Expires: 2001-07-06
Abstract
Abstract A method is disclosed for the determination of an antigen in an immunochemical reaction whereby the antigen has to form a bond with at least two antibody molecules. In the determination method at least two different sorts of monoclonal antibodies are used directed against the same antigen to be determined.
Inventors
- 👤 Anna Maria Gezina Bosch
- 👤 Antonius Hermanus W. M. Schuurs
- 👤 Wilhelmus Johannes H. M. Stevens
- 👤 Bauke Klaas Van Weemen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 02:31
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; G01N33/541; C; C07; C07K; C07K16/00; C07K16/18; C07K16/26; G01N33/86; G01N2333/00; G01N2333/81; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/968
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
43 words
Method for determining antigens by means of an immunochemical reaction, whereby the antigen must enter into a bond with at least two antibody molecules, characterised in that hereby two or more different sorts of monoclonal antibodies are used directed against the same antigen.
Claim 2
Dependent Claim
43 words
Method for determining antigens by means of an immunochemical reaction, whereby the antigen must enter into a bond with at least two antibody molecules, characterised in that hereby two or more different sorts of monoclonal antibodies are used directed against the same antigen.
Claim 3
Dependent Claim
47 words
Method according to claim 1, characterised in that use is made of a reagent consisting of a mixture of two or more batches of particles, each coated with a different monoclonal antibody against the antigen to be determined, and which agglutinates in the presence of the antigen.
Claim 4
Dependent Claim
44 words
Method according to claim 1, characterised in that use is made of a reagent consisting of particles which are coated with a mixture of two or more monoclonal antibodies against the antigen to be determined and which agglutinate in the presence of the antigen.
Claim 5
Dependent Claim
50 words
Method according to claim 1, characterised in that use is made as reagent both of a monoclonal antibody rendered insoluble and a marked monoclonal antibody, whereby these monoclonal antibodies are not of the same origin, and whereby the antigen to be determined brings about a bond between the two reagents.
Claim 6
Dependent Claim
55 words
Method according to claim 1, characterised in that use is made as reagent both of a mixture of two or more different monoclonal antibodies which have been rendered insoluble together with a marked mixture of two or more different monoclonal antibodies, whereby the antigen to be determined brings about a bond between the two reagents.
Claim 7
Dependent Claim
61 words
Test kit, consisting of: a) one monoclonal antibody or some different sorts of monoclonal antibodies, directed against the same antigen and marked with a certain label, b) one monoclonal antibody different from that of a), or some different sorts of monoclonal antibodies directed against the same antigen and either rendered insoluble or marked with a certain label, c) other suitable reagents.
Claim 8
Dependent Claim
61 words
Test kit, consisting of: a) one monoclonal antibody or some different sorts of monoclonal antibodies, directed against the same antigen and marked with a certain label, b) one monoclonal antibody different from that of a), or some different sorts of monoclonal antibodies directed against the same antigen and either rendered insoluble or marked with a certain label, c) other suitable reagents.
Claim 9
Dependent Claim
58 words
Test kit, consisting of: a) a batch of particles, each coated with different monoclonal antibodies directed against the different antigens to be determined in the sample,, b) a batch of particles,.each coated with monoclonal antibodies directed against the same antigens, whereby these antigens are of a different kind than those mentioned under a), c) other . suitable reagents.
Claim 10
Dependent Claim
58 words
Test kit, consisting of: a) a batch of particles, each coated with different monoclonal antibodies directed against the different antigens to be determined in the sample,, b) a batch of particles,.each coated with monoclonal antibodies directed against the same antigens, whereby these antigens are of a different kind than those mentioned under a), c) other . suitable reagents.
References Cited (Backward Citations)
No citation records indexed.
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