US4829011A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Agglutination assay
Assignee: Roche Diagnostics Operations Inc
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Filing: 1987-08-27
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Granted: 1989-05-09
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Expires: 2007-08-27
Abstract
Abstract A method of detecting the presence and/or amount (concentration) of an analyte in a sample is provided by forming a reaction medium containing (1) a sample; (2) a plurality of particles having a binding pair member bound to their surfaces; and (3) a monovalent complementary partner to the binding pair member to which is bound an analyte mimic or analyte binding partner; and detecting the presence or amount of agglutination of the particles in the reaction medium. In some cases a polyvalent receptor capable of binding to the analyte (and to the analyte mimic, if present) is also present in the reaction medium.
Inventors
- 👤 Ian Gibbons
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 04:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54306; G01N33/54313; Y; Y10; Y10S; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
98 words
A method of detecting the presence or amount of an analyte in a sample, which comprises: forming a reaction medium containing (1) a sample; (2) a plurality of particles having a binding pair member bound to their surfaces; and (3) an analyte mimic or analyte binding partner bound to a monovalent complementary partner to said binding pair member; detecting agglutination of said particles in said reaction medium; and correlating agglutination with presence of said analyte In said sample if said method is a direct assay and with absence of said analyte if said method is a competitive assay.
Claim 2
Independent Claim
98 words
A method of detecting the presence or amount of an analyte in a sample, which comprises: forming a reaction medium containing (1) a sample; (2) a plurality of particles having a binding pair member bound to their surfaces; and (3) an analyte mimic or analyte binding partner bound to a monovalent complementary partner to said binding pair member; detecting agglutination of said particles in said reaction medium; and correlating agglutination with presence of said analyte In said sample if said method is a direct assay and with absence of said analyte if said method is a competitive assay.
Claim 3
Dependent Claim
37 words
The method of claim 1, wherein an analyte mimic is bound to said monovalent complementary partner and said reaction medium further comprises a polyvalent receptor capable of binding both with said analyte and with said analyte mimic.
Claim 4
Dependent Claim
18 words
The method of claim 2, wherein said analyte comprises a single binding site reactive with said polyvalent receptor.
Claim 5
Dependent Claim
17 words
The method of claim 1, wherein an analyte binding partner is bound to said monovalent complementary partner.
Claim 6
Dependent Claim
20 words
The method of claim 4, wherein said analyte comprises a plurality of binding sites reactive with said analyte binding partner.
Claim 7
Dependent Claim
48 words
The method of claim 4, wherein said analyte comprises a single binding site reactive with said analyte binding partner and said reaction medium further comprises a polyvalent receptor capable of binding to a second site on said analyte different from the site reactive with said analyte binding partner.
Claim 8
Dependent Claim
12 words
The method of claim 1, wherein said sample naturally contains said particles.
Claim 9
Dependent Claim
11 words
The method of claim 4, wherein said sample is whole blood.
Claim 10
Dependent Claim
14 words
The method of claim 1, wherein said complementary partner is a monovalent immunoglobulin fragment.
Claim 11
Dependent Claim
17 words
The method of claim 9, wherein said fragment is specific for a red blood cell surface antigen.
Claim 12
Dependent Claim
12 words
The method of claim 1, wherein said particle is a latex particle.
Claim 13
Dependent Claim
17 words
The method of claim 2, wherein more than one analyte mimic is bound to said complementary partner.
Claim 14
Dependent Claim
13 words
The method of claim 1, wherein said polyvalent receptor is a polyvalent immunoglobulin.
Claim 15
Dependent Claim
14 words
The method of claim 1, wherein said binding pair member is a polyvalent immunoglobulin.
Claim 16
Dependent Claim
43 words
The method of claim 1, wherein said monovalent complementary partner comprises a mixture of monovalent complementary partners of different types, each type having the same monovalent binding specifity but comprising an analyte binding partner specific for a different determinant of the same antigen.
Claim 17
Dependent Claim
12 words
The method of claim 1, wherein detecting agglutination is by visual inspection.
Claim 18
Dependent Claim
21 words
The method of claim 1, wherein detecting agglutination is by electronically measuring light transmitted, reflected, or scattered by said reaction medium.
Claim 19
Dependent Claim
71 words
A regeant combination for use in the detection of an analyte, comprising (1) a monovalent complementary partner to a member of a complementary binding pair, said complementary partner having bound thereto an analyte mimic or analyte binding partner, and (2) either (a) a polyvalent receptor capable of binding with said analyte and with said analyte mimic or (b) a plurality of particles having said binding pair member bound to their surfaces.
Claim 20
Dependent Claim
71 words
A regeant combination for use in the detection of an analyte, comprising (1) a monovalent complementary partner to a member of a complementary binding pair, said complementary partner having bound thereto an analyte mimic or analyte binding partner, and (2) either (a) a polyvalent receptor capable of binding with said analyte and with said analyte mimic or (b) a plurality of particles having said binding pair member bound to their surfaces.
Claim 21
Dependent Claim
14 words
The reagent combination of claim 18, wherein said complementary partner is a monovalent immunoglobulin.
Claim 22
Dependent Claim
27 words
The reagent combination of claim 18, wherein said analyte mimic bound to said complementary partner is a molecule of said analyte covalently attached to a monovlaent immunoglobulin.
Claim 23
Dependent Claim
29 words
The reagent combination of claim 18, wherein each member of said reagent pair is present as a dry reagent in an analytical device designed for the measurement of agglutination.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-08 | 1992-11-07 | 1993-05-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-08 | 1996-11-07 | 1997-05-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-08 | 2000-11-07 | 2001-05-09 | $7700.00 | Window Closed / Lapsed |