Patent JPS5544772A successfully refreshed from Google Patents.
US3843776A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic method and means therefor
Assignee: Leo AB
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Filing: 1971-05-06
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Granted: 1974-10-22
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Expires: 1991-05-06
Abstract
Abstract Indirect hemagglutination is utilized in a serological method to detect the presence of a bacterial infection by means of antibacteriophage antibodies in the serum of a patient. The antibodies are caused to react with known bacteriophages which are fixed on aldehyde-treated erythrocytes and the resulting hemagglutination serves as an indicium of the types of antibodies that are present. Bacteriophages are adsorbed on aliphatic aldehyde-treated erythrocytes at a temperature of at least about 45* C. and fixed by a subsequent aliphatic aldehyde treatment.
Inventors
- 👤 C Kamme
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 19:24
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56911; G01N33/56938; Y; Y10; Y10S; Y10S436/00; Y10S436/802; Y10S436/811; Y10S436/826
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
93 words
A METHOD IN WHICH ERYTHROCYTES BEARING PHAGES DERIVED FROM BACTERIA ARE PRODUCED WHICH COMPRISES CONTACTING ERYTHROCYTES WITH AN AQUEOUS C1 TO C5 ALIPHATIC ALDEHYDE SOLUTION, CONTACTING THE RESULTING ALDEHYDE-TREATED ERYTHROCYES WITH STAPHYLOCOCCAL BACTERIOPHAGES AT A TEMPERATURE OF ABOUT 45* C TO ABOUT 60*C FOR A PERIOD OF TIME SUFFICIENT TO ADHERE SAID PHAGES TO SAID ERYTHROCYTES, AND THEN CONTACTING THE PHAGEBEARING ERYTHROCYTES WITH AN AQUEOUS C1 TO C5 ALIPHATIC ALDEHYDE SOLUTION AT A TEMPERATURE OF ABOUT 35*C TO ABOUT 60*C FOR A PERIOD OF TIME SUFFICIENT TO FIX SAID PHAGES TO SAID ERYTHROCYTES.
Claim 2
Independent Claim
93 words
A METHOD IN WHICH ERYTHROCYTES BEARING PHAGES DERIVED FROM BACTERIA ARE PRODUCED WHICH COMPRISES CONTACTING ERYTHROCYTES WITH AN AQUEOUS C1 TO C5 ALIPHATIC ALDEHYDE SOLUTION, CONTACTING THE RESULTING ALDEHYDE-TREATED ERYTHROCYES WITH STAPHYLOCOCCAL BACTERIOPHAGES AT A TEMPERATURE OF ABOUT 45* C TO ABOUT 60*C FOR A PERIOD OF TIME SUFFICIENT TO ADHERE SAID PHAGES TO SAID ERYTHROCYTES, AND THEN CONTACTING THE PHAGEBEARING ERYTHROCYTES WITH AN AQUEOUS C1 TO C5 ALIPHATIC ALDEHYDE SOLUTION AT A TEMPERATURE OF ABOUT 35*C TO ABOUT 60*C FOR A PERIOD OF TIME SUFFICIENT TO FIX SAID PHAGES TO SAID ERYTHROCYTES.
Claim 3
Dependent Claim
35 words
The method of claim 1 in which the aldehydetreated erythrocytes are contacted with the bacteriophages in the presence of a sugar or protein selected from the group consisting of glucose, peptone, serum globulin, and albumin.
Claim 4
Dependent Claim
31 words
The method of claim 1 in which the aldehydetreated erythrocytes are contacted with the bacteriophages in the presence of the broth from the nutrient medium in which the phage is produced.
Claim 5
Dependent Claim
17 words
The method of claim 1 wherein the erythrocytes are derived from sheep and the aldehyde is formaldehyde.
Claim 6
Dependent Claim
28 words
The method of claim 1 wherein the contacting of the aldehyde-treated erythrocytes with a phage is carried out at a temperature of about 50* to about 60* C.
Claim 7
Dependent Claim
22 words
The method of claim 1 wherein the aldehyde-treated erythrocytes are further contacted with tannic acid prior to the contact with a phage.
Claim 8
Dependent Claim
13 words
Erythrocytes having Staphylococcal phages fixed thereon, prepared by the method of claim 1.
Claim 9
Dependent Claim
122 words
A method of diagnosis for a bacterial infection caused by bacteria of the genus Staphylococcus having bacteriophages indigenous thereto, which comprises providing an aliquot of serum from a living animal body, pre-treating said serum with erythrocytes so as to remove heterophilic antibodies from the serum, and admixing said pre-treated serum with erythrocytes having a phage of a type known to be indigenous to bacteria of the genus Staphylococcus fixed thereon prepared by the method of claim 1 in amounts sufficient to cause hemagglutination of the erythrocytes if antibodies active against said phage are present in said serum, the agglutination serving to indicate that a bacterial infection by bacteria having indigenous phages of a similar type is present in said living animal body.
Claim 10
Dependent Claim
29 words
The diagnostic method of claim 8 wherein an aliquot of the serum is serially diluted and the diluted portions thereof are admixed with known amounts of said phage-bearing erythrocytes.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-10-21 | 1978-04-22 | 1978-10-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-10-21 | 1982-04-22 | 1982-10-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-10-21 | 1986-04-22 | 1986-10-22 | $7700.00 | Window Closed / Lapsed |