US4820647A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for detecting a metal ion in an aqueous environment
Assignee: Roche Diagnostics Operations Inc
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Filing: 1986-12-03
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Granted: 1989-04-11
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Expires: 2006-12-03
Abstract
Abstract A method for detecting the presence of a metal ion in an aqueous environment, which comprises contacting a micelle-forming material, a hydrophobic or amphiphilic dye, and a hydrophobic ionophore capable of selectively binding the ion with a sample of the aqueous environment to form a mixture and measuring a change in absorbance of light by the dye. Compositions method are also disclosed.
Inventors
- 👤 Ian Gibbons
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 09:45
CPC Classifications
G; G01; G01N; G01N31/00; G01N31/22
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
87 words
A method for detecting the presence of a metal ion in an aqueous environment, comprising: contacting a micelle-forming material, a hydrophobic or amphiphilic dye, and a hydrophilic ionophore capable of selectively binding said ion, wherein said hydrophobic ionophore is a molecule separate from said dye, with a sample of said aqueous environment to form a micelle-containing mixture, and detecting a change in absorbance of light by said dye in said mixture, wherein said change in absorbance is dependent on binding of said hydrophobic ionophore to said ion.
Claim 2
Independent Claim
87 words
A method for detecting the presence of a metal ion in an aqueous environment, comprising: contacting a micelle-forming material, a hydrophobic or amphiphilic dye, and a hydrophilic ionophore capable of selectively binding said ion, wherein said hydrophobic ionophore is a molecule separate from said dye, with a sample of said aqueous environment to form a micelle-containing mixture, and detecting a change in absorbance of light by said dye in said mixture, wherein said change in absorbance is dependent on binding of said hydrophobic ionophore to said ion.
Claim 3
Dependent Claim
26 words
The method of claim 1, wherein said dye has two or more aromatic rings and a phenolic hydroxyl with a pK in the range of 3-13.
Claim 4
Dependent Claim
28 words
The method of claim 1, wherein said dye is phenolphthalein, bromocresol purple, cresophthalein, chlorophenol red, tetrabromophenol blue, thymophthalein, 5-aminoeosin, eosin-5-maleamic acid, 5-(N-dodecanoyl)aminoeosin, 5-(N-hexadecanoyl)-aminoeosin, 7-(n-decyl)-2-methyl-4-(3',5'-dichlorophen-4'-one)indonaphthol, or acid blue #45.
Claim 5
Dependent Claim
25 words
The method of claim 1, wherein said micelle-forming material is present as an aqueous micelle contining composition comprising an aqueous buffer prior to said contacting.
Claim 6
Dependent Claim
38 words
The method of claim 1, wherein said micelle-forming material comprises about 0.05-3% by weight of said mixture, said dye comprises about 0.005-1% by weight of said mixture, and said ionophore comprises about 0.01-1% by weight of said mixture.
Claim 7
Dependent Claim
13 words
The method of claim 1, wherein said micelle-forming material is an organic surfactant.
Claim 8
Dependent Claim
23 words
The method of claim 6, wherein said surfactant is sodium cholate, sodium deoxycholate, a bile salt, sodium dodecyl sulfate, or a mixture thereof.
Claim 9
Dependent Claim
12 words
The method of claim 1, wherein said ionophore is a crown ether.
Claim 10
Dependent Claim
17 words
The method of claim 8, wherein said ionophore is 2,3-naphtho-15-crown-5 or dicyclohexano-18-crown-6 and said ion is potassium.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-04-10 | 1992-10-10 | 1993-04-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-04-10 | 1996-10-10 | 1997-04-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-04-10 | 2000-10-10 | 2001-04-11 | $7700.00 | Window Closed / Lapsed |