US4816397A
Expired / Lapsed
US
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Multichain polypeptides or proteins and processes for their production
Assignee: Celltech R&D Ltd
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Filing: 1984-03-23
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Granted: 1989-03-28
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Expires: 2004-03-23
Abstract
Abstract Multichain polypeptides or proteins and processes for their production in cells of host organisms which have been transformed by recombinant DNA techniques. According to a first aspect of the present invention, there is provided a process for producing a heterologous multichain polypeptide or protein in a single host cell, which comprises transforming the host cell with DNA sequences coding for each of the polypeptide chains and expressing said polypeptide chains in said transformed host cell. According to another aspect of the present invention there is provided as a product of recombinant DNA technology an Ig heavy or light chain or fragment thereof having an intact variable domain. The invention also provides a process for increasing the level of protein expression in a transformed host cell and vectors and transformed host cells for use in the processes.
Inventors
- 👤 Michael A. Boss
- 👤 John H. Kenten
- 👤 John S. Emtage
- 👤 Clive R. Wood
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 01:56
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/46; C07K16/461; C07K16/462; A; A61; A61P; A61P7/00; B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01D15/3809; C12; C12N; C12N15/00; C12N15/09; C12N15/63; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; A61K; A61K38/00; C07K2317/00; C07K2317/20; C07K2317/24
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
105 words
A process for producing an Ig molecule or an immunologially functional Ig fragment comprising at least the variable domains of the Ig heavy and light chains, in a single host cell, comprising the steps of: (i) transforming said single host cell with a first DNA sequence encoding at least the variable domain of the Ig heavy chain and a second DNA sequence encoding at least the variable domain of the Ig light chain, and (ii) independently expessing said first DNA sequence and said second DNA sequence so that said Ig heavy and light chains are produced as separate molecules in said transformed single host cell.
Claim 2
Dependent Claim
105 words
A process for producing an Ig molecule or an immunologially functional Ig fragment comprising at least the variable domains of the Ig heavy and light chains, in a single host cell, comprising the steps of: (i) transforming said single host cell with a first DNA sequence encoding at least the variable domain of the Ig heavy chain and a second DNA sequence encoding at least the variable domain of the Ig light chain, and (ii) independently expessing said first DNA sequence and said second DNA sequence so that said Ig heavy and light chains are produced as separate molecules in said transformed single host cell.
Claim 3
Dependent Claim
18 words
The process according to claim 1 wherein said first and second DNA sequences are present in different vectors.
Claim 4
Dependent Claim
19 words
The process according to claim 1 wherein said first and second DNA sequences are present in a single vector.
Claim 5
Dependent Claim
14 words
A process according to claim 2 wherein the or each vector is a plasmid.
Claim 6
Dependent Claim
18 words
A process according to claim 4, wherein the plasmid is col E1, pcR1, pBR322, RP4 or phage DNA.
Claim 7
Dependent Claim
16 words
A process according to claim 1 wherein the host cell is a baceterium or a yeast.
Claim 8
Dependent Claim
18 words
A process according to claim 6, wherein the host cell is E. Coli, B. subtilis or S. cerevisiae.
Claim 9
Dependent Claim
24 words
A process according to claim 7, wherein the host cell is E. Coli strain HB101, X1776, X2887, PS410, MRC 1 RV308, B or E1035.
Claim 10
Dependent Claim
31 words
A process according to claim 1 wherein the Ig heavy and light chains are expressed in the host cell and secreted therefrom as an immunologically functional Ig molecule or Ig fragment.
Claim 11
Dependent Claim
40 words
A process according to claim 1 wherein the Ig heavy and light chains are produced in insoluble or membrane bound form and are solubilised and allowed to refold in solution to form an immunologically functional Ig molecule or Ig fragment.
Claim 12
Dependent Claim
19 words
A process according to claim 1, wherein the DNA sequences code for the complete Ig heavy and light chains.
Claim 13
Dependent Claim
40 words
A process according to claim 1, wherein said first or said second DNA sequence further encodes at least one constant domain, wherein the constant domain is derived from the same source as the variable domain to which it is attached.
Claim 14
Dependent Claim
45 words
A process according to claim 1, wherein said first or said second DNA sequence further encodes at least one constant domain, wherein the constant domain is derived from a source different from that from which the variable domain to which it is attached is derived.
Claim 15
Dependent Claim
23 words
A process according to claim 1, wherein said first and second DNA sequences are derived from one or more monoclonal antibody producing hybridomas.
Claim 16
Dependent Claim
53 words
A vector comprising a first DNA sequence encoding at last a variable domain of an Ig heavy chain and a second DNA sequence encoding at least a variable domain of an Ig light chain wherein said first DNA sequence and said second DNA sequence are located in said vector at different insertion sites.
Claim 17
Dependent Claim
53 words
A vector comprising a first DNA sequence encoding at last a variable domain of an Ig heavy chain and a second DNA sequence encoding at least a variable domain of an Ig light chain wherein said first DNA sequence and said second DNA sequence are located in said vector at different insertion sites.
Claim 18
Dependent Claim
10 words
A vector according to claim 15, which is a plasmid.
Claim 19
Dependent Claim
11 words
A host cell transformed with a vector according to claims 15.
Claim 20
Dependent Claim
53 words
A transformed host cell comprising at least two vectors, at least one of said vectors comprising a DNA sequence encoding at least a variable domain of an Ig heavy chain and at least another one of said vectors comprising a DNA sequence encoding at least the variable domain of an Ig light chain.
Claim 21
Dependent Claim
53 words
A transformed host cell comprising at least two vectors, at least one of said vectors comprising a DNA sequence encoding at least a variable domain of an Ig heavy chain and at least another one of said vectors comprising a DNA sequence encoding at least the variable domain of an Ig light chain.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-03-27 | 1992-09-26 | 1993-03-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-03-27 | 1996-09-26 | 1997-03-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-03-27 | 2000-09-26 | 2001-03-28 | $7700.00 | Window Closed / Lapsed |