EP0037723A2
Expired / Lapsed
EP
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Expression of hormone genomic clones
Assignee: University of California
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Filing: 1981-04-03
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Granted: —
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Expires: 2001-04-03
Abstract
Abstract Cloned DNA segments comprise a gene coding for a human polypeptide hormone and are derived from human genome and are capable of being expressed in a eucaryotic cell. The human polypeptide hormone may be human insulin. The cloned DNA segments may be comprised in a DNA trans- fervector.
Inventors
- 👤 Graeme Bell
- 👤 Howard Michael Goodman
- 👤 William J. Rutter
- 👤 Barbara Cordell
- 👤 Orgad Laub
- 👤 Leslie Rall
- 👤 Raymond L. Institute De Recherche En Pictet
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 22:24
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/62
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
24 words
A cloned DNA segment derived from the human genome, comprising the gene for a polypeptide hormone, capable of being expressed in a eucaryotic cell.
Claim 2
Dependent Claim
24 words
A cloned DNA segment derived from the human genome, comprising the gene for a polypeptide hormone, capable of being expressed in a eucaryotic cell.
Claim 3
Dependent Claim
13 words
The cloned DNA segment of claim 1, wherein the polypeptide hormone is insulin.
Claim 4
Dependent Claim
10 words
The cloned DNA segment of claim 1, designated gHI 12.5.
Claim 5
Dependent Claim
10 words
The cloned DNA segment of claim 1, designated gHI 14.1
Claim 6
Dependent Claim
12 words
A DNA transfer vector comprising the cloned DNA segment of claim 1.
Claim 7
Dependent Claim
17 words
The DNA transfer vector of claim 5, wherein the cloned DNA segment comprises the human insulin gene.
Claim 8
Dependent Claim
18 words
The DNA transfer vector of claim 6, comprising pBR322 with gHI 12.5 inserted at the EcoRI site thereof.
Claim 9
Dependent Claim
12 words
A microorganism containing and replicating the DNA transfer vector of claim 1.
Claim 10
Dependent Claim
20 words
A microorganism according to claim 8, wherein the transfer vector comprises a cloned DNA segment comprising the human insulin gene.
Claim 11
Dependent Claim
23 words
The microorganism according to claim 9, wherein the transfer vector comprises the plasmid pBR322 with gHI 12.5 inserted at the EcoRI site thereof.
Claim 12
Dependent Claim
9 words
The microorganism of claim 10, comprising E. coli RRI.
Claim 13
Dependent Claim
24 words
A eucaryotic cell strain, grown in culture, transformed by the cloned DNA segment of claim 1, said cell strain capable of expressing said gene.
Claim 14
Dependent Claim
16 words
The cell strain of claim 12, wherein the cloned DNA segment comprises the gene for insulin.
Claim 15
Dependent Claim
15 words
The cell strain of claim 12 or 13, comprising mouse Ltk - aprt - cells.
Claim 16
Dependent Claim
22 words
The cell strain of claim 14, wherein the cloned DNA segment is selected from the group comprising gHI 12.5 and gHI 14.5.
Claim 17
Dependent Claim
24 words
A cell culture comprising the eucaryotic cell strain of claim 12 and a growth medium suitable for growth and replication of said cell strain.
Claim 18
Dependent Claim
23 words
A cell culture according to claim 16, wherein said cell strain is transformed by a cloned DNA segment comprising the gene for insulin.
Claim 19
Dependent Claim
15 words
The cell culture of claim 16 or 17, comprising mouse Ltk - aprt - cells.
Claim 20
Dependent Claim
66 words
A process for making human insulin comprising the steps of: (a) preparing the cloned DNA segment of claim 2, 3 or 4 containing the human insulin gene; (b) transforming a eucaryotic cell strain grown in culture with said cloned DNA segment; (c) selecting transformed cells capable of expressing said human insulin gene; (d) making an extract of said cells; and (e) purifying insulin from said extract.
Claim 21
Dependent Claim
24 words
The DNA transfer Vector of claim 6, comprising a portion of the SV40 genome and a portion of gHI 12.5 containing the insulin gene.
Claim 22
Dependent Claim
63 words
A process for preparing a DNA transfer vector for replicating a DNA segment containing a gene coding for a human polypeptide hormone comprising isolating a DNA segment containing said gene and inserting said segment into a DNA transfer vector characterized in that said DNA segment is derived from the human genome and said gene is capable of being expressed in a eucaryotic cell.
Claim 23
Dependent Claim
63 words
A process for preparing a DNA transfer vector for replicating a DNA segment containing a gene coding for a human polypeptide hormone comprising isolating a DNA segment containing said gene and inserting said segment into a DNA transfer vector characterized in that said DNA segment is derived from the human genome and said gene is capable of being expressed in a eucaryotic cell.
Claim 24
Dependent Claim
11 words
The process of claim 1, wherein the polypeptide hormone is insulin.
Claim 25
Dependent Claim
13 words
The process of claim 1, wherein the DNA segment is designated gHI 12.5.
Claim 26
Dependent Claim
13 words
The process of claim 1, wherein the DNA segment is designated gHI 14.5.
Claim 27
Dependent Claim
16 words
The process of claim 3, wherein gHI 12.5 is inserted at the EcoRI site of pBR323.
Claim 28
Dependent Claim
17 words
The process of claim 3, wherein the DNA transfer vector comprises a portion of the SV40 genome.
Claim 29
Dependent Claim
37 words
A process for preparing a microorganism containing and replicating a DNA transfer vector comprising transforming a microorganism with said transfer vector characterized in that said transfer vector is prepared according to any of claims 1 to 6.
Claim 30
Dependent Claim
12 words
The process of claim 7 wherein said microorganism is .E. coli RRI.
Claim 31
Dependent Claim
47 words
A process for preparing a eucaryotic cell strain, grown in culture, containing a DNA transfer vector and capable of expressing said transfer vector comprising transforming a microorganism with said transfer vector, characterized in that said transfer vector is prepared according to any of claims 1 to 6.
Claim 32
Dependent Claim
14 words
The process of claim 9 wherein said cell strain comprises mouse Ltk aprt cells.
Claim 33
Dependent Claim
38 words
A process for preparing a cell culture comprising a cell strain and a growth medium suitable for growth and replication of said cell strain characterized in that said cell strain is prepared according to claim 9 or 10.
Claim 34
Dependent Claim
65 words
A process for preparing human insulin comprising: (a) preparing a transfer vector containing a gene for human insulin prepared according to any of claims 2 to 6; (b) transforming a eucaryotic cell strain grown in culture with said transfer vector; (c) selecting transformed cells capable of expressing said insulin gene; (d) making an extract of said cells, and (e) purifying human insulin from said extract.
References Cited (Backward Citations)
No citation records indexed.
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