Patent US4808520A successfully refreshed from Google Patents.
US4808520A
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Labelling of oligonucleotides
Assignee: MILES INC. A CORP. OF INDIANA
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Filing: 1985-03-15
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Granted: 1989-02-28
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Expires: 2005-03-15
Abstract
Abstract A method is provided of making an oligonucleotide which comprises hybridizing (a) a shorter fragment of the desired oligonucleotide with (b) a nucleic acid fragment longer than (a) and complementary to the desired oligonucleotide, one of (a) and (b) is linked to an organic radical which differentiates its physical properties relative to the other of (a) and (b), contacting the hybridized material with an enzyme and nucleoside triphosphates whereby the shorter fragment is extended in one direction until it is substantially coterminal with the complementary nucleic acid fragment, denaturing the hybridized material and separating lengthened (a) from (b).
Inventors
- 👤 Nanibhushan Dattagupta
- 👤 William J. Knowles
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 16:18
CPC Classifications
C; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07H21/00
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
98 words
A method of making an oligonucleotide probe which consists essentially of hybridizing a template primer pair of oligonucleotides of equal lengths, each of said oligonucleotides of the template primer pair being shorter than the desired probe and being complementary with one another over only a portion of their lengths at opposite ends, contacting the hybridized oligonucleotide pair with an enzyme and one or more nucleoside triphosphates, the one or more nucleoside triphosphates permitting extension of one of the oligonucleotides, whereby one of the oligonucleotides is extended selectively in one direction until it is coterminal with the other oligonucleotide.
Claim 2
Independent Claim
98 words
A method of making an oligonucleotide probe which consists essentially of hybridizing a template primer pair of oligonucleotides of equal lengths, each of said oligonucleotides of the template primer pair being shorter than the desired probe and being complementary with one another over only a portion of their lengths at opposite ends, contacting the hybridized oligonucleotide pair with an enzyme and one or more nucleoside triphosphates, the one or more nucleoside triphosphates permitting extension of one of the oligonucleotides, whereby one of the oligonucleotides is extended selectively in one direction until it is coterminal with the other oligonucleotide.
Claim 3
Dependent Claim
22 words
A method according to claim 1, wherein the nucleoside triphosphates include a label, whereby the extended fragment is linked to said label.
Claim 4
Dependent Claim
11 words
A method according to claim 1, wherein the label is radioactive.
Claim 5
Dependent Claim
27 words
A method according to claim 1, wherein the fragments comprise 5' GCAGACTTCTCCXC 3' GAAGAGGYGTCCTC wherein one of X and Y is T and the other is A.
Claim 6
Dependent Claim
12 words
A method according to claim 1, wherein the enzyme is DNA polymerase.
Claim 7
Dependent Claim
12 words
A method according to claim 1, wherein the enzyme is reverse transcriptase.
Claim 8
Dependent Claim
20 words
A method according to claim 1, wherein the hybridized region of the template primer pair is of nine nucleotide residues.
References Cited (Backward Citations)
EP0184056A2
Cited
EP0200362B1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-02-28 | 1992-08-29 | 1993-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-02-28 | 1996-08-29 | 1997-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-02-28 | 2000-08-29 | 2001-02-28 | $7700.00 | Window Closed / Lapsed |