EP0184056A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Large scale production of DNA probes
Assignee: Molecular Diagnostics Inc
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Filing: 1985-11-16
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Granted: —
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Expires: 2005-11-16
Abstract
Abstract A process for production of a single strand of a nucleic acid comprising a covalently linking to a solid substrate a polynucleotide complementary to the desired strand, hybridizing said polynucleotide with a oligonucleotide, extending the oligonucleotide in direction away from said substrate, denaturing the hybridized polynucleotide and extended oligonucleotide, thereby to free the extended oligonucleotide from the solid substrate, and separating the extended oligonucleotide. The product can be used for making analytical probes.
Inventors
- 👤 Nanibhushan Dr. Dattagupta
- 👤 Peter Dr. Rae
- 👤 Donald Dr. Crothers
- 👤 Thomas Dr. Barnett
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 08:45
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6844; C12Q1/686; C12Q1/6876
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
32 words
A structure for producing a specific nucleic acid strand, comprising a solid substrate, a single-stranded polynucleotide covalently linked at one end to the solid substrate, and an oligonucleotide hybridized to the polynucleotide.
Claim 2
Dependent Claim
32 words
A structure for producing a specific nucleic acid strand, comprising a solid substrate, a single-stranded polynucleotide covalently linked at one end to the solid substrate, and an oligonucleotide hybridized to the polynucleotide.
Claim 3
Dependent Claim
15 words
A structure according to claim 1, wherein the oligonucleotide comprises at least five nucleotide residues.
Claim 4
Dependent Claim
22 words
A structure according to any of claims 1 and 2, wherein the 3'-end of the polynucleotide is linked to the solid substrate.
Claim 5
Dependent Claim
17 words
A structure according to any of claims 1 to 3, wherein the link contains a -N-C- bond.
Claim 6
Dependent Claim
50 words
A structure for producing a specific nucleic acid strand, comprising a solid substrate, a single-stranded first polynucleotide covalently linked at one end to the solid substrate, and a second polynucleotide hybridized to the first polynucleotide, one end of one polynucleotide being complementary to the other end of the second polynucleotide.
Claim 7
Dependent Claim
50 words
A structure for producing a specific nucleic acid strand, comprising a solid substrate, a single-stranded first polynucleotide covalently linked at one end to the solid substrate, and a second polynucleotide hybridized to the first polynucleotide, one end of one polynucleotide being complementary to the other end of the second polynucleotide.
Claim 8
Dependent Claim
25 words
A structure according to claim 5, wherein the 3'-end of the first polynucleotide is linked to the solid substrate, the link containing a -N-C- bond.
Claim 9
Dependent Claim
63 words
A process for production of a single strand of a nucleic acid comprising covalently linking to a solid substrate a polynucleotide complementary to the desired strand, hybridizing said polynucleotide with an oligonucleotide, extending the oligonucleotide in direction away from said substrate, denaturing the hybridized polynucleotide and extended oligonucleotide, thereby to free the extended oligonucleotide from the solid substrate, and separating the extended oligonucleotide.
Claim 10
Dependent Claim
63 words
A process for production of a single strand of a nucleic acid comprising covalently linking to a solid substrate a polynucleotide complementary to the desired strand, hybridizing said polynucleotide with an oligonucleotide, extending the oligonucleotide in direction away from said substrate, denaturing the hybridized polynucleotide and extended oligonucleotide, thereby to free the extended oligonucleotide from the solid substrate, and separating the extended oligonucleotide.
Claim 11
Dependent Claim
20 words
A process according to claim 7, including the further step of recycling the solid support carrying the covalently linked polynucleotide.
References Cited (Backward Citations)
No citation records indexed.
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