US4797624A
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Method and apparatus for editing particle produced electrical pulses
Assignee: Beckman Coulter Inc
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Filing: 1986-05-30
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Granted: 1989-01-10
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Expires: 2006-05-30
Abstract
Abstract Particle produced pulses are evaluated on an individual basis for shape symmetry, with significantly asymmetric pulses, becoming subject to editing, i.e. exclusion. Pulse symmetry is determined by measuring the areas under the pulse before and after the first pulse amplitude peak and then by comparing those pulse areas with respect to pre-established limits which are not based upon any of the pulses then being evaluated.
Inventors
- 👤 Harvey J. Dunstan
- 👤 John G. Harfield
- 👤 Paul Knight
- 👤 Harvey J. Podgorney
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 11:31
CPC Classifications
G; G01; G01R; G01R29/00; G01R29/02; G01N; G01N15/00; G01N15/10; G01N15/1031; G01N15/12; G01N15/131; G01N15/132; G01R19/00; G01R19/04; G01R29/027; G01R29/0273; H; H03; H03K; H03K5/00; H03K5/153; H03K5/1532
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
41 words
A method for editing particle produced electrical pulses, said method comprising the steps of: determining the symmetry of each pulse, comparing said symmetry with respect to acceptable limits thereof, and editing each pulse which lies outside of the acceptable symmetry limits.
Claim 2
Independent Claim
41 words
A method for editing particle produced electrical pulses, said method comprising the steps of: determining the symmetry of each pulse, comparing said symmetry with respect to acceptable limits thereof, and editing each pulse which lies outside of the acceptable symmetry limits.
Claim 3
Dependent Claim
26 words
The method according to claim 1 in which said step of determining the symmetry is accomplished by measuring the areas of different portions of a pulse.
Claim 4
Dependent Claim
28 words
The method according to claim 2 in which said areas are of two different pulse portions which lie on opposite sides of a peak amplitude of the pulse.
Claim 5
Dependent Claim
19 words
The method according to claim 3 in which said peak amplitude is the first peak amplitude of the pulse.
Claim 6
Independent Claim
35 words
The method according to any one of claims 1, 2, or 3 including the step of establishing said acceptable limits in a manner which is not based upon any pulse being processed by this method.
Claim 7
Independent Claim
30 words
The method according to any one of claims 2, 3, or 4 in which said step of comparing compares the different pulse portion areas with respect to said acceptable limits.
Claim 8
Dependent Claim
51 words
The method according to claim 1 in which said acceptable limits are defined by the general formula YB<A<ZB, in which A is the area of one portion of the pulse, and YB and ZB are equal to B, the area of an other portion of the pulse, multiplied by different constants.
Claim 9
Dependent Claim
15 words
The method according to claim 7 in which Y approximates 0.5 and Z approximates 1.4.
Claim 10
Dependent Claim
22 words
The method according to claim 7 in which the area A lies on one side of the peak amplitude of the pulse.
Claim 11
Independent Claim
32 words
The method according to any one of claims 7, 8, or 9 in which the area A is the area of the pulse which develops after the first peak of the pulse.
Claim 12
Independent Claim
45 words
The method according to any one of claims 7, 8, or 9 in which B is the area of said other portion of the pulse and is developed before the first peak of the pulse for comparing with the area limits of the area A.
Claim 13
Independent Claim
50 words
The method according to any one of claims 1, 2, or 7 which has the additional step of comparing each pulse with a pre-established minimal acceptable width at a threshold amplitude, whereby any pulse failing to exceed this pre-established minimal width cannot succeed in passing through this method for editing.
Claim 14
Independent Claim
25 words
The method of any one of claims 1, 2, or 7 further comprising the step of generating said pulse by use of the Coulter principle.
Claim 15
Independent Claim
84 words
An apparatus for editing particle produced pulses, said apparatus comprising: pulse symmetry determining means for determining the symmetry of each pulse, symmetry limits defining means for defining pre-established limits of pulse symmetry, comparing means coupled to be responsive to both said pulse symmetry determining means and said symmetry limits defining means for comparing at least a portion of each pulse with respect to pulse symmetry and for generating an output signal indicative of such comparison, said output signal being useful for pulse editing determination.
Claim 16
Independent Claim
84 words
An apparatus for editing particle produced pulses, said apparatus comprising: pulse symmetry determining means for determining the symmetry of each pulse, symmetry limits defining means for defining pre-established limits of pulse symmetry, comparing means coupled to be responsive to both said pulse symmetry determining means and said symmetry limits defining means for comparing at least a portion of each pulse with respect to pulse symmetry and for generating an output signal indicative of such comparison, said output signal being useful for pulse editing determination.
Claim 17
Dependent Claim
78 words
The apparatus according to claim 14 in which said pulse symmetry determining means measures the area of two different portions of the pulse and feeds one of the area portions directly to said comparing means, and said symmetry limits defining means is interposed between said pulse symmetry determining means and said comparing means for receiving the other of the area portions and scaling it with respect to the pre-established symmetry limits prior to comparison by said comparing means.
Claim 18
Dependent Claim
47 words
The apparatus according to claim 15 in which said pulse symmetry determining means includes pulse peak detecting means which defines the two different pulse portions as said one being that which is after the pulse peak and said other being that which is before that pulse peak.
Claim 19
Dependent Claim
65 words
The apparatus according to claim 15 or 16 in which said symmetry limits defining means includes scaling means operative with the form YB<A<ZB, in which: A is the area of the one pulse portion, Y approximates 0.5, and Z approximates 1.4; whereby the thus scaled value of the area B is compared with the area A, which is the area of said one pulse portion.
Claim 20
Independent Claim
41 words
The apparatus according to any one of claims 14, 15, or 16 in which said symmetry limits defining means includes pre-set means for fixing the pre-established limits such that those limits are not influenced by pulses being processed by said apparatus.
Claim 21
Dependent Claim
33 words
The apparatus according to any one of claim 14, 15, or 16 in which said particle produced pulses are generated by particles passing through sensing aperture means operating according to the Coulter principle.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-01-10 | 1992-07-11 | 1993-01-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-01-10 | 1996-07-11 | 1997-01-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-01-10 | 2000-07-11 | 2001-01-10 | $7700.00 | Window Closed / Lapsed |