US4790919A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparation of electrophoresis gel material
Assignee: EIDP Inc
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Filing: 1984-06-28
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Granted: 1988-12-13
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Expires: 2004-06-28
Abstract
Abstract Describes a process for controlling the polymerization and cross-linked density of electrophoretic gel products useful for separation of bioorganic molecules, which process utilizes photoinitiation. Photoinitiated polymerized gels afford the desired advantages of being ultra thin and having a high electrophoretic resolution with programmable porosity profiles.
Inventors
- 👤 Charles Baylor, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 02:04
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; C; C08; C08F; C08F20/00; C08F20/02; C08F20/52; C08F20/54; C08F20/56; Y; Y10; Y10S; Y10S522/00; Y10S522/91
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
90 words
A porous electrophoretic gel product comprising an aqueous-swelled porous polymer matrix, formed of polymerized and cross-linked monomers and a photoinitiator, which defines a volume, the product characterized by having a constant atomic composition over its volume, being stable, having a controlled, electrophoretic resolving capacity that is reproducible from gel to gel for the electrophoretic separation of charged macromolecular substances, having length, width and thickness dimensions, and having a porosity gradient along one of the length and width dimensions and uniform porosity along the thickness dimension which dimension is relativey thin.
Claim 2
Dependent Claim
90 words
A porous electrophoretic gel product comprising an aqueous-swelled porous polymer matrix, formed of polymerized and cross-linked monomers and a photoinitiator, which defines a volume, the product characterized by having a constant atomic composition over its volume, being stable, having a controlled, electrophoretic resolving capacity that is reproducible from gel to gel for the electrophoretic separation of charged macromolecular substances, having length, width and thickness dimensions, and having a porosity gradient along one of the length and width dimensions and uniform porosity along the thickness dimension which dimension is relativey thin.
Claim 3
Dependent Claim
41 words
The gel product set forth in claim 1, in which the monomers are acrylamide or acrylamide derivatives and cross linking monomers selected from the group consisting of N,N,-methylene-bis-acrylamide (BIS), N,N'-diallyltartramide (DATD), ethylene diacrylate, N,N'-bis-acrylyl cystamine, N,N'-(1,2-dihydroxyethylene)-bis-acrylamide and TEOTA (polyxyethyl trimethylolpropane triacrylate).
Claim 4
Dependent Claim
40 words
The gel product set forth in claim 1, wherein the polymerization is initiated by homogeneous photopolymerization using water soluble ultraviolet sensitive photoinitiators or heterogeneous photopolymerization in which the ultraviolet sensitive initiator is suspended or dispersed in the aqueous photopolymerization solution.
Claim 5
Dependent Claim
20 words
The gel product set forth in claim 3 wherein the weight/volume percentage of polyacrylamide or polyacrylamide derivative is about 5%.
Claim 6
Dependent Claim
23 words
The gel product set forth in claim 3 wherein the acrylamide monomer consists essentially of from about 3% weight/volume to about 30% weight/volume.
Claim 7
Dependent Claim
36 words
The gel product set forth in claim 1 wherein the product consists essentially of 3 to 30% weight/volume of acrylamide monomer, and about 0.3 to 1.2% weight/volume of a water soluble or water dispersible photoinitiating systems.
Claim 8
Dependent Claim
22 words
The gel product set forth in claim 6 wherein about 2 to 15% weight/volume of the acrylamide monomer is a cross-linkable monomer.
Claim 9
Dependent Claim
14 words
The gel product set forth in claim 7 wherein the porosity gradient is linear.
Claim 10
Dependent Claim
14 words
The gel product set forth in claim 7 wherein the porosity gradient is logarithmic.
Claim 11
Dependent Claim
20 words
The gel product set forth in claim 7 wherein the porosity gradient can be represented by a real mathematical function.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-12-13 | 1992-06-13 | 1992-12-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-12-13 | 1996-06-13 | 1996-12-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-12-13 | 2000-06-13 | 2000-12-13 | $7700.00 | Window Closed / Lapsed |