Patent FR858932A successfully refreshed from Google Patents.
Patent US4789628A successfully refreshed from Google Patents.
US4789628A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Devices for carrying out ligand/anti-ligand assays, methods of using such devices and diagnostic reagents and kits incorporating such devices
Assignee: VXR Inc 217 READ STREET PORTLAND MAINE 04103 A CORP OF DE
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Filing: 1986-06-16
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Granted: 1988-12-06
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Expires: 2006-06-16
Abstract
Abstract This invention relates to devices and to methods, diagnostic reagents and kits embodying such devices for use in ligand/anti-ligand assays and the qualitative or semi-quantitative detection of a substance of interest thereby through use of light or photon detecting systems such as reflectometers, colorimeters, fluorimeters and the human eye. Such devices, methods and kits have applications in the biologic research and medical fileds for assaying drugs, hormones, peptides, proteins, enzymes, nucleic acids, antibodies, haptens, antibiotics, viri, infectious agents, tumor markers and the like, in the laboratory, the physicians' office, the veterinarian's office and the home.
Inventors
- 👤 P. N. Nayak
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 20:56
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/5302; G01N33/5304; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S435/975; Y10S436/00; Y10S436/80; Y10S436/807; Y10S436/808; Y10S436/809; Y10S436/81
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
70 words
A device for assaying a sample for the presence of a ligand by forming within the device a reaction product of the ligand with at least one anti-ligand therefor, said device comprising: a plastic member defining at least one well having a bottom; and a plurality of spaced projections extending upward from the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween.
Claim 2
Independent Claim
70 words
A device for assaying a sample for the presence of a ligand by forming within the device a reaction product of the ligand with at least one anti-ligand therefor, said device comprising: a plastic member defining at least one well having a bottom; and a plurality of spaced projections extending upward from the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween.
Claim 3
Dependent Claim
17 words
A device according to claim 1 wherein the projections are pyramidal, columnar, conical, rectangular, cylindrical or dome-shaped.
Claim 4
Dependent Claim
18 words
A device according to claim 1 wherein the well further comprises at least one immobilized ligand or anti-ligand.
Claim 5
Dependent Claim
27 words
A device according to claim 3 wherein the ligand is a drug, hormone, peptide, protein, enzyme, nucleic acid, antibody, hapten, antibiotic, receptor, virus, hormone or infectious agent.
Claim 6
Dependent Claim
19 words
A device according to claim 1 wherein the well bottom comprises one or more immobilized chromogens, luminogens or fluorogens.
Claim 7
Dependent Claim
12 words
A device according to claim 1 wherein the plastic member is molded.
Claim 8
Dependent Claim
14 words
A device according to claim 1 wherein the plastic member is transparent or translucent.
Claim 9
Dependent Claim
17 words
A device according to claim 1 wherein the well bottom further comprises reactive chemical groups grafted thereto.
Claim 10
Dependent Claim
14 words
A device according to claim 1 wherein the sides of the well are contoured.
Claim 11
Dependent Claim
18 words
A device according to claim 1 wherein the plastic member comprises polyethylene, polypropylene, polystyrene, polycarbonate, polysulfone or polymethylmethacrylate.
Claim 12
Dependent Claim
18 words
A device according to claim 1 wherein the capacity of the well bottom is at least 0.05 ml.
Claim 13
Dependent Claim
23 words
A device according to claim 1 wherein the surface area of the well bottom is increased by at least a factor of 2.
Claim 14
Dependent Claim
11 words
A device according to claim 1 having a plurality of wells.
Claim 15
Independent Claim
135 words
A method for assaying one or more samples for the presence of at least one ligand with the aid of a device for assaying one or more samples for the presence of a ligand by forming within the device a detectable reaction product of the ligand with at least one anti-ligand therefor and detecting the reaction product, the device comprising: a plastic member defining at least one well having a bottom, the well comprising at least one immobilized anti-ligand; and a plurality of spaced projections extending upward from the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween; the method comprising: (1) introducing the sample into the well; (2) forming in the well a ligand/anti-ligand reaction product; and (3) detecting the ligand/anti-ligand reaction product capable of detection.
Claim 16
Independent Claim
135 words
A method for assaying one or more samples for the presence of at least one ligand with the aid of a device for assaying one or more samples for the presence of a ligand by forming within the device a detectable reaction product of the ligand with at least one anti-ligand therefor and detecting the reaction product, the device comprising: a plastic member defining at least one well having a bottom, the well comprising at least one immobilized anti-ligand; and a plurality of spaced projections extending upward from the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween; the method comprising: (1) introducing the sample into the well; (2) forming in the well a ligand/anti-ligand reaction product; and (3) detecting the ligand/anti-ligand reaction product capable of detection.
Claim 17
Dependent Claim
18 words
The method of claim 14, wherein the method further comprises washing the well between one or more steps.
Claim 18
Dependent Claim
17 words
A method according to claim 14 wherein the ligand comprises an antigen and the anti-ligand comprises antibody.
Claim 19
Dependent Claim
86 words
The method of claim 14 wherein the well bottom comprises a first antibody; and step 3 comprises the steps of: (i) adding to the well a solution comprising a conjugate of an enzyme with a second antibody and forming a reaction product comprising the first antibody, the antigen and the second antibody; (ii) washing the well; and (iii) adding to the well a second solution comprising a substrate for the enzyme which reacts in the presence of the enzyme to produce a color or color change.
Claim 20
Independent Claim
134 words
A method for assaying one or more samples for the presence of at least one ligand with the aid of a device for assaying one or more samples for the presence of a ligand by forming within the device a detectable reaction product of the ligand with at least one anti-ligand therefor and detecting the reaction product, the device comprising: a plastic member defining at least one well having a bottom, the well comprising at least one immobilized ligand; and a plurality of spaced projections extending upward from the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween; the method comprising: (1) introducing the sample into the well; (2) forming in the well a ligand/anti-ligand reaction product; and (3) detecting the reaction product capable of detection.
Claim 21
Independent Claim
134 words
A method for assaying one or more samples for the presence of at least one ligand with the aid of a device for assaying one or more samples for the presence of a ligand by forming within the device a detectable reaction product of the ligand with at least one anti-ligand therefor and detecting the reaction product, the device comprising: a plastic member defining at least one well having a bottom, the well comprising at least one immobilized ligand; and a plurality of spaced projections extending upward from the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween; the method comprising: (1) introducing the sample into the well; (2) forming in the well a ligand/anti-ligand reaction product; and (3) detecting the reaction product capable of detection.
Claim 22
Dependent Claim
18 words
The method of claim 14, wherein the method further comprises washing the well between one or more steps.
Claim 23
Dependent Claim
18 words
A method according to claim 14 wherein the ligand comprises an antigen and the anti-ligand comprises an antibody.
Claim 24
Dependent Claim
90 words
A kit comprising, in combination: (a) a device for assaying a sample for the presence of a ligand by forming within the device a reaction product of the ligand with an anti-ligand therefor, said device comprising: a plastic member defining at least one well having a bottom, the well comprising an immobilized anti-ligand; and a plurality of spaced projections extending upward from the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween; (b) the ligand, wherein the ligand is capable of detection.
Claim 25
Dependent Claim
90 words
A kit comprising, in combination: (a) a device for assaying a sample for the presence of a ligand by forming within the device a reaction product of the ligand with an anti-ligand therefor, said device comprising: a plastic member defining at least one well having a bottom, the well comprising an immobilized anti-ligand; and a plurality of spaced projections extending upward from the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween; (b) the ligand, wherein the ligand is capable of detection.
Claim 26
Dependent Claim
95 words
A kit comprising, in combination: (a) a device for assaying a sample for the presence of a ligand by forming within the device a reaction product of the ligand with a first and second anti-ligand therefor, said device comprising: a plastic member defining at least one well having a bottom, the well comprising the first anti-ligand; and a plurality of spaced projections extending upward from the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween; and (b) a second anti-ligand, the second anti-ligand being capable of detection.
Claim 27
Dependent Claim
95 words
A kit comprising, in combination: (a) a device for assaying a sample for the presence of a ligand by forming within the device a reaction product of the ligand with a first and second anti-ligand therefor, said device comprising: a plastic member defining at least one well having a bottom, the well comprising the first anti-ligand; and a plurality of spaced projections extending upward from the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween; and (b) a second anti-ligand, the second anti-ligand being capable of detection.
Claim 28
Dependent Claim
91 words
A kit comprising, in combination: (a) a device for assaying a sample for the presence of a ligand by forming within the device a reaction product of the ligand with an anti-ligand therefor, said device comprising: a plastic member defining at least one well having a bottom, the well comprising at least one ligand; and a plurality of spaced projections extending upward form the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween; and (b) an anti-ligand, the anti-ligand being capable of detection.
Claim 29
Dependent Claim
91 words
A kit comprising, in combination: (a) a device for assaying a sample for the presence of a ligand by forming within the device a reaction product of the ligand with an anti-ligand therefor, said device comprising: a plastic member defining at least one well having a bottom, the well comprising at least one ligand; and a plurality of spaced projections extending upward form the well bottom to increase the surface area thereof, the projections being spaced to define interconnecting channels therebetween; and (b) an anti-ligand, the anti-ligand being capable of detection.
Claim 30
Dependent Claim
29 words
The kit of claim 21, wherein the ligand is detectable by light or radiation emission, absorption or fluoresence or by measuring the amount of light reflected from the well.
Claim 31
Dependent Claim
30 words
The kit of claim 22, wherein the second anti-ligand is dectectable by light or radiation emission, absorption or fluoresence or by measuring the amount of light reflected from the well.
Claim 32
Dependent Claim
29 words
The kit of claim 24, wherein the anit-ligand is dectectable by light or radiation emission, absorption or fluoresence or by measuring the amount of light reflected from the well.
References Cited (Backward Citations)
US4317810A
Cited
US4090849A
Cited
US4147752A
Cited
US4197287A
Cited
US4197361A
Cited
US4146365A
Cited
US4280992A
Cited
US4225575A
Cited
US4628036A
Cited
US4599315A
Cited
US4661460A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-12-06 | 1992-06-06 | 1992-12-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-12-06 | 1996-06-06 | 1996-12-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-12-06 | 2000-06-06 | 2000-12-06 | $7700.00 | Window Closed / Lapsed |