US4786591A — Process for determining the binding capacity of t…

Claim 1

Independent Claim 100 words

A process for determining the binding capacity of thyroxin binding globulin (TBG) comprising incubating a serum sample containing TBG with T 4 or T 3 bound via a spacer to an enzyme label and immobilized antibody which specifically binds to TBG under conditions favoring binding of TBG to labeled T 4 or T 3 and formation of complexes between said immobilized antibody and TBG bound to labeled T 4 or T 3 , separating free and immobilized phrases and measuring label in one of the phases as a measurement of the binding capacity of thyroxin binding globulin in said sample.

Claim 2

Dependent Claim 100 words

A process for determining the binding capacity of thyroxin binding globulin (TBG) comprising incubating a serum sample containing TBG with T 4 or T 3 bound via a spacer to an enzyme label and immobilized antibody which specifically binds to TBG under conditions favoring binding of TBG to labeled T 4 or T 3 and formation of complexes between said immobilized antibody and TBG bound to labeled T 4 or T 3 , separating free and immobilized phrases and measuring label in one of the phases as a measurement of the binding capacity of thyroxin binding globulin in said sample.

Claim 3

Dependent Claim 99 words

A process for determining the binding capacity of thyroxin binding globulin (TBG) comprising incubating a serum sample containing TBG with T 4 bound via a spacer to an enzyme label without addition of unlabeled T 4 and with immobilized antibody which specifically binds to labeled T 4 unbound to TBG under conditions favoring binding of TBG to labeled T 4 and formation of complexes between labeled T 4 and said immobilized antibody, separating free and immobilized phases and measuring label in one of said phases as a measurement of the binding capacity of thyroxin binding globulin in said sample.

Claim 4

Dependent Claim 99 words

A process for determining the binding capacity of thyroxin binding globulin (TBG) comprising incubating a serum sample containing TBG with T 4 bound via a spacer to an enzyme label without addition of unlabeled T 4 and with immobilized antibody which specifically binds to labeled T 4 unbound to TBG under conditions favoring binding of TBG to labeled T 4 and formation of complexes between labeled T 4 and said immobilized antibody, separating free and immobilized phases and measuring label in one of said phases as a measurement of the binding capacity of thyroxin binding globulin in said sample.

Claim 5

Dependent Claim 22 words

Process according to claim 1 or 2, wherein a spacer is used with a length of from 3 to 8 carbon atoms.

Claim 6

Dependent Claim 15 words

Process according to claim 3, wherein aminobutyric acid or glycylglycine is used as the spacer.

Claim 7

Dependent Claim 56 words

Process according to claim 1 or 2 comprising carrying out said process in two stages the first stage comprising incubating the sample with the enzyme labelled T 3 or T 4 and the second stage comprises incubating said sample and enzyme labelled T 3 or T 4 with the immobilized antibody against T 4 or TBG.

Claim 8

Dependent Claim 29 words

Process according to claim 5 wherein said immobilized antibody is an antibody against T 4 and the second incubation stage is carried out for not longer than 5 minutes.

Claim 9

Dependent Claim 30 words

Process according to claim 1 or 2, wherein said enzyme labelled T 4 is a T 4 -β-galactosidase conjugate, the presence of which is determined in a color forming reaction.

Claim 10

Dependent Claim 106 words

A process for determining the binding capacity of thyroxin binding globulin (TBG) with T 4 or T 3 bound via a spacer to an enzyme, an antibody specific for TBG and an immobilized antibody which specifically binds to the Fc portion of said TBG specific antibody under conditions favoring binding of TBG to said labeled T 4 or T 3 , said TBG specific antibody to TBG, and said immobilized Fc specific antibody to said TBG specific antibody, separating free and immobilized phases and measuring the label in one of said phases as a measure of the binding capacity of thyroxin binding globulin in said sample.

Claim 11

Dependent Claim 106 words

A process for determining the binding capacity of thyroxin binding globulin (TBG) with T 4 or T 3 bound via a spacer to an enzyme, an antibody specific for TBG and an immobilized antibody which specifically binds to the Fc portion of said TBG specific antibody under conditions favoring binding of TBG to said labeled T 4 or T 3 , said TBG specific antibody to TBG, and said immobilized Fc specific antibody to said TBG specific antibody, separating free and immobilized phases and measuring the label in one of said phases as a measure of the binding capacity of thyroxin binding globulin in said sample.

Claim 12

Dependent Claim 110 words

A process for determining the binding capacity of thyroxin binding globulin (TBG) comprising incubating a serum sample containing TBG with T 4 bound via a spacer to an enzyme, an antibody specific for labeled T 4 and an immobilized antibody which specifically binds to the Fc portion of said T 4 specific antibody under conditions favoring binding of T 4 to TBG, T 4 to said T 4 specific antibody and said immobilized Fc specific antibody to said T 4 specific antibody, separating free and immobilized phases and measuring the label in one of said phases as a measure of the binding capacity of thyroxin binding globulin in said sample.

Claim 13

Dependent Claim 110 words

A process for determining the binding capacity of thyroxin binding globulin (TBG) comprising incubating a serum sample containing TBG with T 4 bound via a spacer to an enzyme, an antibody specific for labeled T 4 and an immobilized antibody which specifically binds to the Fc portion of said T 4 specific antibody under conditions favoring binding of T 4 to TBG, T 4 to said T 4 specific antibody and said immobilized Fc specific antibody to said T 4 specific antibody, separating free and immobilized phases and measuring the label in one of said phases as a measure of the binding capacity of thyroxin binding globulin in said sample.

Claim 14

Dependent Claim 32 words

Process according to claims 2, 8 or 9 wherein, the measurement obtained is compared to a control or standard which comprises human serum containing a known amount of monoclonal anti-T 4 antibodies.

Claim 15

Dependent Claim 119 words

Process according to claim 10 comprising incubating the control or standard human serum containing the monoclonal anti T 4 -antibodies with a known amount of enzyme labelled T 4 or T 3 under conditions favoring formation of complexes between said antibodies and said T 4 or T 3 , contacting said human serum containing labeled T 4 or T 3 with a solid phase to which are fixed antibodies which bind to Fc γ part of the monoclonal anti-T 4 -antibody under conditions favoring formation of complexes between said Fc γ specific antibody and said monoclonal anti-T 4 antibody and separating the liquid phase from the solid phase and measuring the labelling enzyme contained in one of said phases.

Claim 16

Dependent Claim 25 words

Process according to claim 10 wherein said immobilized antibody specifically binds to a common epitope of said monoclonal anti-T 4 antibody and an anti-TBG antibody.

Claim 17

Dependent Claim 17 words

Process according to claim 8 or 9 wherein said antibody specific for TBG is a monoclonal antibody.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1991-11-22	1992-05-23	1992-11-22	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1995-11-22	1996-05-23	1996-11-22	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	1999-11-22	2000-05-23	2000-11-22	$7700.00	Window Closed / Lapsed

Process for determining the binding capacity of thyroxin-binding globulin

Abstract

Inventors

Bibliographic Details

Claims Analysis

References Cited (Backward Citations)

USPTO Annuity & Maintenance Windows

Assigned Categories & Tags

Assign Category Tag