US4476228A
Expired / Lapsed
US
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Determination of unsaturated thyroxine binding protein sites using fluorescence polarization techniques
Assignee: Abbott Laboratories
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Filing: 1982-11-08
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Granted: 1984-10-09
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Expires: 2002-11-08
Abstract
Abstract This invention encompasses a method for measuring unsaturated thyroxine binding protein sites in a sample comprising intermixing with the sample an effective amount of fluorescent labeled tracer having binding affinity for thyroxine binding proteins and then measuring the amount of tracer bound to the thyroxine binding protein using fluorescence polarization techniques.
Inventors
- 👤 Roy F. Huchzermeier
- 👤 Thomas G. Spring
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 16:01
CPC Classifications
C; C07; C07D; C07D493/00; C07D493/02; C07D493/10; C07D311/00; C07D311/02; C07D311/78; C07D311/80; C07D311/82; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
100 words
A method for determining unsaturated thyroxine binding protein sites in a sample containing thyroxine binding proteins, comprising: (a) treating the sample with a composition comprising a nonfluorescent surfactant in a buffered medium having a pH greater than 7.0; (b) contacting the treated sample with a fluorescent labeled tracer wherein said fluorescent labeled tracer has a preferential binding affinity for thyroxine binding globulin, thereby forming a complex between the thyroxine binding proteins and fluorescent labeled tracer; and (c) determining the amount of complex formed utilizing fluorescence polarization techniques, as a measure of the unsaturated thyroxine binding protein sites in the sample.
Claim 2
Independent Claim
100 words
A method for determining unsaturated thyroxine binding protein sites in a sample containing thyroxine binding proteins, comprising: (a) treating the sample with a composition comprising a nonfluorescent surfactant in a buffered medium having a pH greater than 7.0; (b) contacting the treated sample with a fluorescent labeled tracer wherein said fluorescent labeled tracer has a preferential binding affinity for thyroxine binding globulin, thereby forming a complex between the thyroxine binding proteins and fluorescent labeled tracer; and (c) determining the amount of complex formed utilizing fluorescence polarization techniques, as a measure of the unsaturated thyroxine binding protein sites in the sample.
Claim 3
Dependent Claim
14 words
A method according to claim 1 wherein the nonfluorescent surfactant is sodium dodecyl sulfate.
Claim 4
Dependent Claim
19 words
A method according to claim 2 wherein the pH of the buffered media is in the range of 8.0-9.0.
Claim 5
Dependent Claim
12 words
A method according to claim 3 wherein the buffer is sodium bicarbonate.
Claim 6
Dependent Claim
16 words
A method according to claim 2 wherein the buffered media comprises triethylenediamine in a phosphate buffer.
Claim 7
Dependent Claim
15 words
A method according to claim 1 wherein the fluorescent labeled tracer comprises a thyroxine-fluorescein conjugate.
Claim 8
Dependent Claim
27 words
A method according to claim 6 wherein the thyroxine-fluorescein conjugate is a compound of the formula: ##STR11## wherein m is an integer of from 2 to 8.
Claim 9
Dependent Claim
16 words
A method according to claim 7 wherein m is an integer of from 2 to 4.
Claim 10
Dependent Claim
10 words
A method according to claim 8 wherein m is 3.
Claim 11
Independent Claim
16 words
A method as in any of claims 1-9 wherein steps (a) and (b) are conducted simultaneously.
References Cited (Backward Citations)
US4161515A
Cited
EP0015695A1
Cited
FR2487835A1
Cited
US4347059A
Cited
US4347058A
Cited
DE3205506A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-09 | 1988-04-09 | 1988-10-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-09 | 1992-04-09 | 1992-10-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-09 | 1996-04-09 | 1996-10-09 | $7700.00 | Window Closed / Lapsed |