US4410633A
Expired / Lapsed
US
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Method for the measurement of free thyroxine or 3,5,3'-triiodothyronine in a liquid sample
Assignee: AMERLITE DIAGNOSTICS Ltd A BRITISH Co
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Filing: 1980-09-25
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Granted: 1983-10-18
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Expires: 2000-09-25
Abstract
Abstract An immunoassay for the measurement of free thyroid hormone, i.e., thyroxine or 3,5,3'-triiodothyronine, in a liquid sample in which the thyroid hormone is present in both free and combined states, which immunoassay comprises the steps of: A. combining the sample with a labeled thyroid hormone-horseradish peroxidase conjugate which does not significantly interact with the thyroxine-binding globulin and thyroxine-binding prealbumin originally present in the sample and immobilized antibody which is specific for the thyroid hormone; B. incubating the resulting mixture; C. separating a solid phase from the liquid phase; and D. measuring the amount of labeled thyroid hormone-horseradish peroxidase conjugate present in either phase by means of the activity of the label.
Inventors
- 👤 William Hertl
- 👤 Frank B. Ward
- 👤 Howard H. Weetall
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 04:12
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S435/00; Y10S435/964; Y10S435/966; Y10S436/00; Y10S436/80; Y10S436/804; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
113 words
An immunoassay for the direct measurement of free thyroxine in a liquid sample in which the thyroxine is present in both free and combined states, which immunoassay comprises the steps of: A. combining the sample with a labeled thyroxine-horseradish peroxidase conjugate which does not significantly interact with the thyroxine-binding globulin and thyroxine-binding prealbumin originally present in the sample and immobilized antibody which is specific for thyroxine; B. Incubating the resultant mixture; C. separating a solid phase from the liquid phase; and D. measuring the amount of labeled thyroxine-horseradish peroxidase conjugate present in either phase by means of the activity of the label; in which the conjugate is radiolabeled, fluorescent labeled, or chemiluminescent labeled.
Claim 2
Dependent Claim
113 words
An immunoassay for the direct measurement of free thyroxine in a liquid sample in which the thyroxine is present in both free and combined states, which immunoassay comprises the steps of: A. combining the sample with a labeled thyroxine-horseradish peroxidase conjugate which does not significantly interact with the thyroxine-binding globulin and thyroxine-binding prealbumin originally present in the sample and immobilized antibody which is specific for thyroxine; B. Incubating the resultant mixture; C. separating a solid phase from the liquid phase; and D. measuring the amount of labeled thyroxine-horseradish peroxidase conjugate present in either phase by means of the activity of the label; in which the conjugate is radiolabeled, fluorescent labeled, or chemiluminescent labeled.
Claim 3
Dependent Claim
11 words
The immunoassay of claim 1 in which the conjugate is radiolabeled.
Claim 4
Dependent Claim
14 words
The immunoassay of claim 2 in which the conjugate is labeled with radioactive iodine.
Claim 5
Dependent Claim
14 words
The immunoassay of claim 3 in which the radioactive iodine is I 125 .
Claim 6
Dependent Claim
15 words
The immunoassay of claim 2 in which the antibody is immobilized on an inorganic carrier.
Claim 7
Dependent Claim
15 words
The immunoassay of claim 5 in which the carrier is particulate and relatively finely divided.
Claim 8
Dependent Claim
11 words
The immunoassay of claim 6 in which the carrier is siliceous.
Claim 9
Dependent Claim
11 words
The immunoassay of claim 7 in which the carrier is glass.
Claim 10
Dependent Claim
13 words
The immunoassay of claim 2 in which the carrier is a three-dimensional article.
Claim 11
Dependent Claim
13 words
The immunoassay of claim 9 in which the carrier is a test tube.
Claim 12
Dependent Claim
12 words
The immunoassay of claim 10 in which the test tube is plastic.
Claim 13
Dependent Claim
22 words
The immunoassay of claims 9 or 10 in which the antibody is coated on the lower interior portion of the test tube.
Claim 14
Dependent Claim
101 words
An immunoassay for the measurement of free 3,5,3'-triiodothyronine in a liquid sample in which the 3,5,3'-triiodothryonine is present in both free and combined states, which immunoassay comprises the steps of: A. combining the sample with a radiolabeled 3,5,3'-triiodothyronine-horseradish peroxidase conjugate which does not significantly interact with the thyroxine-binding globulin and thyroxine-binding prealbumin originally present in the sample and immobilized antibody which is specific for 3,5,3'-triiodothyronine; B. incubating the resulting mixture; C. separating a solid phase from the liquid phase; and D. measuring the amount of labeled 3,5,3'-triiodothyronine-horseradish peroxidase conjugate present in either phase by means of the activity of the label.
Claim 15
Dependent Claim
101 words
An immunoassay for the measurement of free 3,5,3'-triiodothyronine in a liquid sample in which the 3,5,3'-triiodothryonine is present in both free and combined states, which immunoassay comprises the steps of: A. combining the sample with a radiolabeled 3,5,3'-triiodothyronine-horseradish peroxidase conjugate which does not significantly interact with the thyroxine-binding globulin and thyroxine-binding prealbumin originally present in the sample and immobilized antibody which is specific for 3,5,3'-triiodothyronine; B. incubating the resulting mixture; C. separating a solid phase from the liquid phase; and D. measuring the amount of labeled 3,5,3'-triiodothyronine-horseradish peroxidase conjugate present in either phase by means of the activity of the label.
Claim 16
Dependent Claim
22 words
The immunoassay of claim 13, in which the antibody is immobilized on an inorganic carrier which is particulate and relatively finely divided.
Claim 17
Dependent Claim
33 words
The immunoassay of claim 13, in which the antibody is immobilized on a carrier which is a test tube wherein the antibody is coated on the lower interior portion of the test tube.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-17 | 1987-04-18 | 1987-10-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-17 | 1991-04-18 | 1991-10-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-17 | 1995-04-18 | 1995-10-18 | $7700.00 | Window Closed / Lapsed |