US4782023A
Expired / Lapsed
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Stabilized horseradish peroxidase conjugate composition
Assignee: Abbott Laboratories
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Filing: 1985-09-13
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Granted: 1988-11-01
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Expires: 2005-09-13
Abstract
Abstract An enzyme conjugate composition comprising an enzyme conjugate, a calcium salt and a polyethylene glycol is disclosed. The enzyme conjugate composition is effectively stabilized by the presence of the calcium salt and the polyethylene glycol.
Inventors
- 👤 Mark A. Anawis
- 👤 Roger E. Lindberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 09:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/56; A61K47/59; A61K47/60; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/105831; Y10T436/108331
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
23 words
A stabilized enzyme conjugate composition, comprising a horseradish peroxidase conjugate, and a stabilizing effective amount of a calcium salt and a polyethylene glycol.
Claim 2
Dependent Claim
23 words
A stabilized enzyme conjugate composition, comprising a horseradish peroxidase conjugate, and a stabilizing effective amount of a calcium salt and a polyethylene glycol.
Claim 3
Dependent Claim
21 words
The composition of claim 1 wherein the polyethylene glycol has an average molecular weight of from about 1,000 to about 20,000.
Claim 4
Dependent Claim
21 words
The composition of claim 2 wherein the polyethylene glycol has an average molecular weight of from about 6,000 to about 8,000.
Claim 5
Dependent Claim
16 words
The composition of claim 1 wherein at least 0.1% of the calcium salt is water soluble.
Claim 6
Dependent Claim
12 words
The composition of claim 1 wherein the calcium salt is calcium chloride.
Claim 7
Dependent Claim
12 words
The composition of claim 1 wherein the calcium salt is calcium propionate.
Claim 8
Dependent Claim
21 words
The composition of claim 1 wherein the calcium salt is present in an amount of from about 0.05% to about 1%.
Claim 9
Dependent Claim
21 words
The composition of claim 7 wherein the calcium salt is present in an amount of from about 0.1% to about 0.3%.
Claim 10
Dependent Claim
21 words
The composition of claim 1 wherein the polyethylene, glycol is present in an amount of from about 0.1% to about 10%.
Claim 11
Dependent Claim
21 words
The composition of claim 9 wherein the polyethylene glycol is present in an amount of from about 1% to about 4%.
Claim 12
Dependent Claim
22 words
The composition of claim 1 wherein the enzyme conjugate is present in an amount of from about 0.01 to about 1.0 ug/ml.
Claim 13
Dependent Claim
40 words
An enzyme conjugate composition containing a horseradish peroxidase conjugate, about 0.05% to about 1% of a calcium salt and about 0.1% to about 10% of a polyethylene glycol with an average molecular weight of from about 1,000 to about 20,000.
Claim 14
Dependent Claim
40 words
An enzyme conjugate composition containing a horseradish peroxidase conjugate, about 0.05% to about 1% of a calcium salt and about 0.1% to about 10% of a polyethylene glycol with an average molecular weight of from about 1,000 to about 20,000.
References Cited (Backward Citations)
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