Enzyme reference composition

Claim 2

An enzyme reference composition of the type comprising: (a) at least one enzyme constituent of known value; (b) from about 20 to about 40 weight percent of at least one alkylene polyol having from 2 to 5 carbon atoms; (c) from about 3 to about 8 grams per deciliter total protein; and (d) from about 60 to about 80 weight percent water; characterized in that said total protein consists essentially of human serum albumin.

Claim 3

The composition of claim 1 having a pH of from about 5.8 to about 8.7.

Claim 4

The composition of claim 1 having a pH of from about 6 to about 8.5.

Claim 5

The composition of claim 1 having a pH of from about 6 to about 7.

Claim 6

The composition of claim 1 having a pH of from about 6.4 to about 6.6.

Claim 7

The composition of any one of claims 2-5 or 1 comprising from about 30 to about 34 weight percent of said alkylene polyol, from about 4 to about 5 grams per deciliter of total protein, and from about 66 to about 70 weight percent water.

Claim 8

The composition of any one of claims 2-5 or 1 wherein at least one of said enzymes is selected from a group consisting of acid phosatase, aldolase, alkaline phosphatase, amylase, cholinesterase, creatine kinase, α-glytamyl transpeptidase, α-hydroxybutyric dehydrogenase, isocitric dehydrogenase, lactic dehydrogenase, leucine aminopeptidase, lipase, alanine amino transferase, and aspartate amino transferase.

Claim 9

The composition of any one of claims 2-5 or 1 wherein said enzyme moiety comprises acid phosphatase, alkaline phosphatase, amylase, creatine kinase, aspartate amino transferase, alanine amino transferase, γ-glutamyl transpeptidase, and lactic dehydrogenase.

Claim 10

The composition of any one of claims 2-5 or 1 comprising: (a) from about 0 to about 350 International Units per liter alkaline phosphatase; (b) from about 0 to about 20 International Units per liter acid phosphatase; (c) from about 0 to about 400 International Units per liter amylase; (d) from about 0 to about 600 International Units per liter creatine kinase; (e) from about 0 to about 200 International Units per liter aspartate amino transferase; (f) from about 0 to about 200 International Units per liter alanine amino transferase; (g) from about 0 to about 300 International Units per liter γ-glutamyl transpeptidase; and (h) from about 0 to about 500 International Units per liter lactic dehydrogenase wherein the enzyme activities are stated for 37° C.

Claim 11

The composition of any one of claims 2-5 or 1 further comprising sodium, potassium, calcium, phosphorous, and magnesium.

Claim 12

The composition of any one of claims 2-5 or 1 comprising: (a) from about 0 to about 350 International Units per liter alkaline phosphatase; (b) from about 0 to about 20 International Units per liter acid phosphatase; (c) from about 0 to about 400 International Units per liter amylase; (d) from about 0 to about 600 International Units per liter creatine kinase; (e) from about 0 to about 200 International Units per liter aspartate amino transferase; (f) from about 0 to about 200 International Units per liter alanine amino transferase; (g) from about 0 to about 300 International Units per liter γ-glytamyl transpeptidase; (h) from about 0 to about 500 International Units per liter lactic dehydrogenase; (i) from about 125 to about 157 milliequivalents per liter sodium; (j) from about 3.5 to about 4.5 millequivalents per liter potassium; (k) from about 8.4 to about 10.6 milligrams per deciliter calcium; (l) from about 1.6 to about 2.4 milligrams per deciliter phosphorous; (m) from about 1.6 to about 2.4 milligrams per deciliter magnesium; and (n) from about 88 to about 112 milliequivalents per liter chloride; wherein the enzyme activities are stated for 37° C.

Claim 13

The composition of any one of claims 2-5 or 1 comprising: (a) from about 0 to about 350 International Units per liter alkaline phosphatase; (b) from about 0 to about 20 International Units per liter acid phosphatase; (c) from about 0 to about 400 International Units per liter amylase; (d) from about 0 to about 600 International Units per liter creatine kinase; (e) from about 0 to about 200 International Units per liter aspartate amino transferase; (f) from about 0 to about 200 International Units per liter alanine amino transferase; (g) from about 0 to about 300 International Units per liter γ-glytamyl transpeptidase; (h) from about 0 to about 500 International Units per liter lactic dehydrogenase; (i) from about 125 to about 157 milliequivalents per liter sodium; (j) from about 3.5 to about 4.5 milliequivalents per liter potassium; (k) from about 8.4 to about 10.6 milligrams per deciliter calcium; (l) from about 1.6 to about 2.4 milligrams per deciliter phosphorous; (m) from about 1.6 to about 2.4 milligrams per deciliter magnesium; and (n) from about 88 to about 112 milliequivalents per liter chloride; wherein the enzyme activities are stated for 37° C.; and wherein said acid phosphatase is derived from human seminal fluid, said alkaline phosphatase is derived from calf intestine; said amylase is derived from pig pancreas; said creatine kinase is derived from monkey muscle; said aspartate amino transferase and alanine amino transferase are derived from pig heart; said γ-glutamyl transpeptidase is derived from pig intestine; and said lactic dehydrogenase is derived from chicken heart.