Patent US4780191A successfully refreshed from Google Patents.
US4780191A
Expired / Lapsed
US
⏱️ 0.0 years remaining
L-glutamine sensor
Assignee: Massachusetts Institute of Technology
•
Filing: 1987-06-26
•
Granted: 1988-10-25
•
Expires: 2007-06-26
Abstract
Abstract Apparatus for measuring L-glutamine in a liquid sample includes a membrane on which are immobilized the enzymes glutaminase and glutamate oxidase, whereby any said L-glutamine in the sample is acted upon by the glutaminase to form glutamic acid, the glutamic acid being acted upon by the glutamate oxidase to form an enzymatic reaction product, the membrane being operatively associated with a sensor capable of sensing the enzymatic reaction product or a compound or element consumed or liberated in the formation or degradation thereof.
Inventors
- 👤 Jean L. Romette
- 👤 Charles L. Cooney
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 15:50
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
83 words
Apparatus for measuring L-glutamine in a liquid sample, comprising a membrane on which are immobilized the enzymes glutaminase and glutamate oxidase, whereby any said L-glutamine in said sample is acted upon by said glutaminase to form glutamic acid,. said glutamic acid being acted upon by said glutamate oxidase to form an enzymatic reaction product, said membrane being operatively associated with a sensor capable of sensing said enzymatic reaction product or a compound or element consumed or liberated in the formation or degradation thereof.
Claim 2
Dependent Claim
83 words
Apparatus for measuring L-glutamine in a liquid sample, comprising a membrane on which are immobilized the enzymes glutaminase and glutamate oxidase, whereby any said L-glutamine in said sample is acted upon by said glutaminase to form glutamic acid,. said glutamic acid being acted upon by said glutamate oxidase to form an enzymatic reaction product, said membrane being operatively associated with a sensor capable of sensing said enzymatic reaction product or a compound or element consumed or liberated in the formation or degradation thereof.
Claim 3
Dependent Claim
20 words
The apparatus of claim 1 wherein said glutaminase and said glutamine oxidase are immobilized in an insolubilized protein containing matrix.
Claim 4
Dependent Claim
17 words
The apparatus of claim 1 wherein said sensor measures dissolved oxygen and said membrane is oxygen permeable.
Claim 5
Dependent Claim
12 words
The sensor of claim 2 wherein said insolubilized protein matrix comprises gelatin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-10-25 | 1992-04-25 | 1992-10-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-25 | 1996-04-25 | 1996-10-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-25 | 2000-04-25 | 2000-10-25 | $7700.00 | Window Closed / Lapsed |