Patent US1684704A successfully refreshed from Google Patents.
Patent US4778762A successfully refreshed from Google Patents.
US4778762A
Expired / Lapsed
US
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Plasmid
Assignee: Ajinomoto Co Inc
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Filing: 1982-10-18
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Granted: 1988-10-18
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Expires: 2002-10-18
Abstract
Abstract A substantially pure plasmid which is characterized by a molecular weight of 3.0±0.1 megadalton and the restriction endonuclease-cleavage map shown in the Figure. This plasmid is capable of propagating in Corynebacteria and due to its size is useful as a vector for the cloning of exogenous genes.
Inventors
- 👤 Kiyoshi Miwa
- 👤 Mahito Terabe
- 👤 Takayasu Tsuchida
- 👤 Masaaki Ishida
- 👤 Shigeru Nakamori
- 👤 Konosuke Sano
- 👤 Haruo Momose
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 18:08
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/08; C12N; C12N1/00; C12N1/20; C12N1/205; C12P13/14; C12R; C12R2001/00; C12R2001/01; C12R2001/13; C12R2001/15; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84; Y10S435/843
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
67 words
A biologically pure composite plasmid capable of propagating in glutamic acid-producing Corynebacteria, comprising: (a) a genetic region derived from a plasmid and capable of amplifying foreign genes; characterized by a molecular weight of 3.0±0.1 megadalton and having the following restriction endonuclease-cleavage map: ______________________________________
Hind III 0/100 Hae III 76
Bcl I 44 Ava I 86 and 99; and
Xba I 55
______________________________________ (b) a foreign gene fragment.
Claim 2
Dependent Claim
67 words
A biologically pure composite plasmid capable of propagating in glutamic acid-producing Corynebacteria, comprising: (a) a genetic region derived from a plasmid and capable of amplifying foreign genes; characterized by a molecular weight of 3.0±0.1 megadalton and having the following restriction endonuclease-cleavage map: ______________________________________
Hind III 0/100 Hae III 76
Bcl I 44 Ava I 86 and 99; and
Xba I 55
______________________________________ (b) a foreign gene fragment.
Claim 3
Dependent Claim
11 words
The biologically pure composite plasmid of claim 1, comprising pAM 330.
Claim 4
Dependent Claim
11 words
The biologically pure composite plasmid of claim 1, comprising pAM 286.
Claim 5
Dependent Claim
19 words
The biologically pure composite plasmid of claim 1, wherein the plasmid is selected from pAM 330 and pAM 286.
Claim 6
Dependent Claim
24 words
The biologically pure composite plasmid of claim 1, wherein the said foreign gene fragment is a genomic DNA fragment of Cornyeform glutamic acid-producing bacteria.
References Cited (Backward Citations)
US4427773A
Cited
EP0058889A1
Cited
US4452890A
Cited
US4442208A
Cited
US4514502A
Cited
US4601983A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-10-18 | 1992-04-18 | 1992-10-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-18 | 1996-04-18 | 1996-10-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-18 | 2000-04-18 | 2000-10-18 | $7700.00 | Window Closed / Lapsed |