US4601983A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Coryneform bacteria carrying recombinant plasmids and their use in the fermentative production of L-threonine and L-isoleucine
Assignee: Ajinomoto Co Inc
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Filing: 1983-06-15
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Granted: 1986-07-22
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Expires: 2003-06-15
Abstract
Abstract A genetic sequence coding for the production of a protein having the activity of homoserine dehydrogenase and having two Pst I cleavage sites in its DNA chain and a molecular weight of 2.24 Md, is incorporated into a vehicle capable of replication in Coryneform bacteria and used to produce L-threonine and L-isoleucine by fermentation.
Inventors
- 👤 Shigeru Nakamori
- 👤 Masaaki Ishida
- 👤 Hiroshi Takagi
- 👤 Kiyoshi Miwa
- 👤 Koichi Ito
- 👤 Konosuke Sano
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 17:49
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77; C12P; C12P13/00; C12P13/04; C12P13/06; C12P13/08; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84; Y10S435/843
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
85 words
A method of producing L-isoleucine by fermentation which comprises: (a) culturing in an appropriate medium a bacterium comprising a Coryneform host containing a vehicle capable of replication in a Coryneform bacterium containing genetic information coding for the production of a protein having the activity of homoserine dehydrogenase, wherein said host is resistant to α-amino-β-hydroxyvaleric acid, and (b) recovering L-isoleucine from said medium; and wherein said Coryneform bacterium is selected from the group consisting of those having the identifying characteristics of FERM BP-270 and FERM BP-271.
Claim 2
Independent Claim
85 words
A method of producing L-isoleucine by fermentation which comprises: (a) culturing in an appropriate medium a bacterium comprising a Coryneform host containing a vehicle capable of replication in a Coryneform bacterium containing genetic information coding for the production of a protein having the activity of homoserine dehydrogenase, wherein said host is resistant to α-amino-β-hydroxyvaleric acid, and (b) recovering L-isoleucine from said medium; and wherein said Coryneform bacterium is selected from the group consisting of those having the identifying characteristics of FERM BP-270 and FERM BP-271.
Claim 3
Independent Claim
85 words
A method of producing L-threonine by fermentation which comprises: (a) culturing in an appropriate medium a bacterium comprising a Coryneform host containing a vehicle capable of replication in a Coryneform bacterium containing genetic information coding for the production of a protein having the activity of homoserine dehydrogenase, wherein said host is resistant to α-amino-β-hydroxyvaleric acid, and (b) recovering L-threonine from said medium; and wherein said Coryneform bacterium is selected from the group consisting of those having the identifying characteristics of FERM BP-270 and FERM BP-271.
Claim 4
Independent Claim
85 words
A method of producing L-threonine by fermentation which comprises: (a) culturing in an appropriate medium a bacterium comprising a Coryneform host containing a vehicle capable of replication in a Coryneform bacterium containing genetic information coding for the production of a protein having the activity of homoserine dehydrogenase, wherein said host is resistant to α-amino-β-hydroxyvaleric acid, and (b) recovering L-threonine from said medium; and wherein said Coryneform bacterium is selected from the group consisting of those having the identifying characteristics of FERM BP-270 and FERM BP-271.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-07-21 | 1990-01-20 | 1990-07-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-07-21 | 1994-01-20 | 1994-07-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-07-21 | 1998-01-20 | 1998-07-22 | $7700.00 | Window Closed / Lapsed |