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EP0077548A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Plasmid

Assignee: Ajinomoto Co Inc
•
Filing: 1982-10-15
•
Granted: —
•
Expires: 2002-10-15
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Abstract

Abstract An essentially pure plasmid is provided, which is characterized by a molecular weight of 3.0 +/- 0.1 megadalton and a restriction endonuclease cleavage chart according to Figure 1. This plasmid may be inserted with a gene which is responsible for the genetic information for the production of a product and the hybrid plasmid may be incorporated into Coryneform glutamic acid producing bacteria as recipient organisms in order to improve their characteristics.

Inventors

  • 👤 Kiyoshi Miwa
  • 👤 Mahito Terabe
  • 👤 Takayasu Tsuchida
  • 👤 Masaaki Ishida
  • 👤 Shigeru Nakamori
  • 👤 Konosuke Sano
  • 👤 Haruo Momose

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-25 21:37
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/14; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N15/63; C12N15/74; C12N15/77; C12P13/08

Claims Analysis

Total Claims: 7
Claim 1
Independent Claim 25 words
A substantially pure plasmid which is characterized by a molecular weight of 3.0 ± 0.1 megadalton and a restriction endonuclease-cleavage chart shown in Figure 1.
Claim 2
Dependent Claim 25 words
A substantially pure plasmid which is characterized by a molecular weight of 3.0 ± 0.1 megadalton and a restriction endonuclease-cleavage chart shown in Figure 1.
Claim 3
Dependent Claim 11 words
A substantially pure plasmid of Claim 1, which is pAM 330.
Claim 4
Dependent Claim 11 words
A substantially pure plasmid of Claim 1, which is pAM 286.
Claim 5
Dependent Claim 35 words
A substantially pure plasmid obtained by insertion of a foreign gene into a plasmid, which is characterized by a molecular weight of 3.0 - 0.1 megadalton and a restriction endonuclease-cleavage chart shown in Figure 1.
Claim 6
Dependent Claim 35 words
A substantially pure plasmid obtained by insertion of a foreign gene into a plasmid, which is characterized by a molecular weight of 3.0 - 0.1 megadalton and a restriction endonuclease-cleavage chart shown in Figure 1.
Claim 7
Dependent Claim 60 words
A process for producing a microorganism from Coryneform glutamic acid producing bacteria , which comprises incorporating into a Coryneform glutamic acid producing bacterium as a recipient a plasmid into which a gene is inserted, which is responsible for the genetic information for the production of a product, characterized in that the.plasmid according to claim 4 is incorporated into the recipient.

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