US4772548A
Expired / Lapsed
US
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Radioisotopicassay using isotope transfer to chelator-target recognition molecule conjugate
Assignee: Enzo Diagnostics Inc
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Filing: 1987-01-23
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Granted: 1988-09-20
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Expires: 2007-01-23
Abstract
Abstract A method of forming a therapeutic or diagnostic agent labeled with a radioactive metal ion, which comprises: contacting an unlabeled therapeutic or diagnostic agent, consisting of a substantially non-metal chelating portion and a chelating portion capable of chelating with the radioactive metal ion, with an ion transfer material having the radioactive metal ion bound thereto and having a binding affinity for the radioactive metal less than the binding affinity of the chelating portion for the radioactive metal ion, wherein prior to contacting the chelating portion is unchelated or is chelated with a second metal having a binding affinity with the chelating portion less than the binding affinity of the radioactive metal ion, whereby a radiolabeled therapeutic or diagnostic agent is formed by the contacting, and separating the radiolabeled therapeutic or diagnostic agent from the ion transfer material, is disclosed along with various components and kits useful in practicing this method and several variations thereof.
Inventors
- 👤 Jannis G. Stavrianpoulos
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 06:32
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/60; A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/0474; A61K51/0478; A61K51/12; G01N33/53; G01N33/531; G01N33/532; G01N33/534; A61K2121/00; A61K2123/00; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
194 words
A radioisotopicassay for detecting an analyte in a sample, which comprises: (1) forming a diagnostic agent labeled with a radioactive metal ion by contacting (i) an unlabeled diagnostic agent comprising (a) a molecularly recognizable portion attached to (b) a chelating portion capable of substantially chelating with said radioactive metal ion, wherein said chelating portion is not a part of said molecularly recognizable portion, with (ii) an ion transfer material having said radioactive metal ion bound thereto and having a binding affinity for said radioactive metal ion less than the binding affinity of said chelating portion for said radioactive metal ion, wherein, prior to said contacting, said chelating portion is unchelated or is chelated with a second metal having a binding affinity with said chelating portion less than the binding affinity of said radioactive metal ion, whereby a radiolabeled diagnostic agent is formed by said contacting; and (2) contacting the sample to be detected when the diagnostic agent so-formed to provide a reaction mixture; (3) separating, if necessary, said ion transfer material and any diagnostic agent which has not reacted with the analyte; and (4) detecting any signal generated from diagnostic agent which has so-reacted.
Claim 2
Dependent Claim
194 words
A radioisotopicassay for detecting an analyte in a sample, which comprises: (1) forming a diagnostic agent labeled with a radioactive metal ion by contacting (i) an unlabeled diagnostic agent comprising (a) a molecularly recognizable portion attached to (b) a chelating portion capable of substantially chelating with said radioactive metal ion, wherein said chelating portion is not a part of said molecularly recognizable portion, with (ii) an ion transfer material having said radioactive metal ion bound thereto and having a binding affinity for said radioactive metal ion less than the binding affinity of said chelating portion for said radioactive metal ion, wherein, prior to said contacting, said chelating portion is unchelated or is chelated with a second metal having a binding affinity with said chelating portion less than the binding affinity of said radioactive metal ion, whereby a radiolabeled diagnostic agent is formed by said contacting; and (2) contacting the sample to be detected when the diagnostic agent so-formed to provide a reaction mixture; (3) separating, if necessary, said ion transfer material and any diagnostic agent which has not reacted with the analyte; and (4) detecting any signal generated from diagnostic agent which has so-reacted.
Claim 3
Dependent Claim
20 words
The radiosiotopicassay of claim 1, wherein said molecularly recognizable portion is a ligand which is specifically bindable with said analyte.
Claim 4
Dependent Claim
46 words
The radioisotopicassay of claim 1, wherein said molecularly recognizable portion is a ligand and which method further comprises the step of contacting the sample with at least one additional component comprising a binding partner for said ligand attached to a specific binding partner for said analyte.
Claim 5
Dependent Claim
48 words
The radioisotopicassay of claim 3, wherein said additional component is bound to a solid substrate, said analyte-specific binding portion thereof is specifically bindable with a first segment of said analyte and the molecularly recognizable portion of said agent is specifically bindable with a second segment of said analyte.
Claim 6
Dependent Claim
32 words
The radioisotopicassay of any of claims 1-3 and 4 wherein at least one of said ligand and the binding partner therefor is an antigen or hapten and the other is an antibody.
Claim 7
Dependent Claim
16 words
The radioisotopicassy of any of claims 1-3 wherein said analyte is bound to a solid substrate.
Claim 8
Dependent Claim
51 words
The radioisotopicassay of claim 1, wherein the molecularly recognizable portion of said agent comprises a ligand and the method further comprises contacting said sample with a multivalent binding partner for said ligand and with at least one additional component comprising a ligand attached to a specific binding partner for said analyte.
Claim 9
Dependent Claim
32 words
The radioisotopicassay of claim 7 wherein said ligand and said specific binding portion of said second component are each biotin or iminobiotin and said multivalent binding portion thereof is avidin or streptavidin.
Claim 10
Dependent Claim
34 words
The radioisotopicassay of claims 1-3, wherein the molecularly recognizable portion of said agent is an antibody, hormone, enzyme, enzyme substrate, enzyme inhibitor, enzyme cofactor, lipid polynucleotide, sequence, virus, virus fragment, cell, or cell fragment.
Claim 11
Dependent Claim
21 words
The radioisotopicassay of claim 9, wherein said molecularly recognizable portion is a virus, virus fragment, bacterial cell, or bacterial cell fragment.
Claim 12
Dependent Claim
13 words
The radioisotopicassay of claim 9, wherein said molecularly recognizable portion is an antibody.
Claim 13
Dependent Claim
14 words
The radioisotopicassay of claim 9, wherein said molecularly recognizable portion is a polynucleotide sequence.
Claim 14
Dependent Claim
13 words
The radioisotopicassay of claim 9 wherein said molecularly recognizable portion is a lipid.
Claim 15
Dependent Claim
19 words
The radioisotopicassay of claim 1, wherein the chelating portion of said agent comprises 1,2-diaminocyclohexaneacetic acid or an analog thereof.
Claim 16
Dependent Claim
115 words
A radioisotopicassay composition for detecting an analyte in a sample, which comprises: (A) an unlabeled diagnostic agent comprising (i) a molecularly recognizable portion attached to (ii) a chelating portion capable of substantially chelating with a radioactive metal ion, wherein said chelating portion is not a part of said molecularly recognizable portion; and is unchelated or is chelated with a second metal having a binding affinity with said chelating portion less than the binding affinity of said radioactive metal ion, (B) an ion transfer material having said radioactive metal ion bound thereto and having a binding affinity for said radioactive metal ion less than the binding affinity of said chelating portion for said radioactive metal ion.
Claim 17
Dependent Claim
115 words
A radioisotopicassay composition for detecting an analyte in a sample, which comprises: (A) an unlabeled diagnostic agent comprising (i) a molecularly recognizable portion attached to (ii) a chelating portion capable of substantially chelating with a radioactive metal ion, wherein said chelating portion is not a part of said molecularly recognizable portion; and is unchelated or is chelated with a second metal having a binding affinity with said chelating portion less than the binding affinity of said radioactive metal ion, (B) an ion transfer material having said radioactive metal ion bound thereto and having a binding affinity for said radioactive metal ion less than the binding affinity of said chelating portion for said radioactive metal ion.
Claim 18
Dependent Claim
20 words
The radioisotopicassay composition of claim 15, wherein said molecularly recognizable portion is a ligand whichis specifically bindable with said analyte.
Claim 19
Dependent Claim
39 words
The radioisotopicassay composition of claim 15, wherein said molecularly recognizable portion is a ligand and which further comprises at least one additional component comprising a binding partner for said ligand attached to a specific binding partner for said analyte.
Claim 20
Dependent Claim
31 words
The radioisotopicassay composition of any of claims 15-17 wherein at least one of said ligand and the binding partner therfor is an antigen or hapten and the other is an antibody.
Claim 21
Dependent Claim
17 words
The radioisotopicassay composition of any of claims 15-17 wherein said analyte is bound to a solid substrate.
Claim 22
Dependent Claim
46 words
The radioisotopicassay composition of claim 15, wherein the molecularly recognizable portion of said agent comprises a ligand and which further comprises a multivalent binding partner for said ligand and at least one additional component comprising a ligand attached to a specific binding partner for said analyte.
Claim 23
Dependent Claim
33 words
The radioisotopicassay composition of claim 20 wherein said ligand and said specific binding portion of said second component are each biotin or iminobiotin and said multivalent binding portion thereof is avidin or streptavidin.
Claim 24
Dependent Claim
35 words
The radioisotopicassay composition of claims 15-17, wherein the molecularly recognizable portion of said agent is an antibody, hormone, enzyme, enzyme substrate, enzyme inhibitor, enzyme cofactor, lipid, polynucleotide, sequence, virus, virus fragment, cell, or cell fragment.
Claim 25
Dependent Claim
22 words
The radioisotopicassay composition of claim 22 wherein said molecularly recognizable portion is a virus, virus fragment, bacterial cell, or bacterial cell fragment.
Claim 26
Dependent Claim
14 words
The radioisotopicassay composition of claim 22 wherein said molecularly recognizable portion is an antibody.
Claim 27
Dependent Claim
15 words
The radioisotopicassay composition of claim 22 wherein said molecularly recognizable portion is a polynucleotide sequence.
Claim 28
Dependent Claim
14 words
The radioisotopicassay composition of claim 22 wherein said molecularly recognizable portion is a lipid.
Claim 29
Dependent Claim
20 words
The radioisotopicassay composition of claim 15 wherein the chelating portion of said agent comprises 1,2-diaminocyclohexaneacetic acid or an analog thereof.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-09-20 | 1992-03-21 | 1992-09-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-09-20 | 1996-03-21 | 1996-09-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-09-20 | 2000-03-21 | 2000-09-20 | $7700.00 | Window Closed / Lapsed |