US4672028A
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US
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Compositions and method for simultaneous multiple array of analytes using radioisotope chelate labels
Assignee: ICN Micromedic Systems Inc
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Filing: 1984-05-23
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Granted: 1987-06-09
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Expires: 2004-05-23
Abstract
Abstract Compounds useful in a simultaneous multiple assay for analytes such as steroids, proteins, peptides, carbohydrates or drugs. The compound or compounds are prepared by labelling an individual analyte with a radioisotope through a chelating agent to form a coordinated compound. The assay uses one or more chelated labelled analytes with one or more labelled analytes wherein each radioisotope is different.
Inventors
- 👤 Douglas R. Olson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 15:08
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/53; G01N33/531; G01N33/532; G01N33/534; G01N33/58; G01N33/60; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/973; Y10S435/975; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/811; Y10S436/813; Y10S436/814; Y10S436/815; Y10S436/816; Y10S436/817; Y10S436/818; Y10S436/82; Y10S436/826
Claims Analysis
Total Claims: 48
Claim 1
Independent Claim
41 words
A composition useful in a simultaneous assay which comprises two or more radioisotope-labeled compounds in a substantially metal-free solution, one or more of said compounds being stable coordinated compounds of the formula radioisotope--chelator--analyte, wherein each radioisotope in said composition is different.
Claim 2
Dependent Claim
41 words
A composition useful in a simultaneous assay which comprises two or more radioisotope-labeled compounds in a substantially metal-free solution, one or more of said compounds being stable coordinated compounds of the formula radioisotope--chelator--analyte, wherein each radioisotope in said composition is different.
Claim 3
Dependent Claim
47 words
The composition according to claim 1 useful in a simultaneous assay wherein the analyte portion of one or more of said stable coordinated compounds is formed from a steroid, a protein, a peptide, a carbohydrate or a drug, and wherein each radioisotope in said composition is different.
Claim 4
Dependent Claim
82 words
The composition according to claim 2 useful in a simultaneous assay wherein the analyte portion of one or more of said stable coordinated compounds is formed from estrogen, progesterone, digoxin, cortisol, 17-hydroxyprogesterone, human chorionic gonadotropin, luteinizing hormone, follicle stimulating hormone, thyroid stimulating hormone, alphafetoprotein, trypsin, T 3 , T 4 , hepatitis associated antigen, carcinoembryonic antigen, ACTH, endorphins, angiotensin, insulin, pneumococcal polysaccharides, cocaine, tetrahydrocannibinol, barbiturate, an amphetamine, gentiamycin, Vitamin B 12 or folate, and wherein each radioisotope in said composition is different.
Claim 5
Dependent Claim
55 words
The composition according to claim 3 useful in a simultaneous assay wherein the analyte portion of one or more of said stable coordinated compounds is formed from luteinizing hormone, T 3 , R 4 , follicle stimulating hormone, thyroid stimulating hormone, Vitamin B 12 or folate, and wherein each radioisotope in said composition is different.
Claim 6
Dependent Claim
43 words
The composition according to claim 4 useful in a simultaneous assay wherein the analyte portion of one or more of said stable coordinated compounds is formed from thyroid stimulating hormone or follicle stimulating hormone, and wherein each radioisotope in said composition is different.
Claim 7
Dependent Claim
74 words
The composition according to claim 5 useful in a simultaneous assay wherein the analyte portion of one or more of said stable coordinated compounds is formed from a chelator of the formula: ##STR3## wherein R 1 is phenyl or substituted phenyl wherein the substituent is selected from NO 2 , NH 2 or SO 3 H and n is an integer of 0 or 1, and wherein each radioisotope in said composition is different.
Claim 8
Dependent Claim
51 words
The composition according to claim 6 useful in a simultaneous assay wherein the analyte portion of one or more of said stable coordinated compounds is formed from a chelating agent selected from ethylenediaminetetraacetic acid, ethylene dinitrilotetraacetic acid, diethylenetriaminepentaacetic acid or derivatives thereof, and wherein each radioisotope in said composition is different.
Claim 9
Dependent Claim
38 words
The composition according to claim 7 useful in a simultaneous assay wherein the analyte portion of one or more of said stable coordinated compounds is formed from ethylenediaminetetraacetic acid, and wherein each radioisotope in said composition is different.
Claim 10
Dependent Claim
43 words
The composition according to claim 8 useful in a simultaneous assay wherein the radioisotope portion of one or more of said stable coordinated compounds is formed from cobalt, iron, iodine, technetium, europium or terbium, and wherein each radioisotope in said composition is different.
Claim 11
Dependent Claim
39 words
The composition according to claim 9 useful in a simultaneous assay wherein the radioisotope portion of one or more of said stable coordinated compounds is formed from cobalt or iron, and wherein each radioisotope in said composition is diifferent.
Claim 12
Dependent Claim
23 words
The composition of claim 1 wherein there are two stable coordinated compounds wherein the analyte portions are formed from carcinoembryonic antigen and β-HCG.
Claim 13
Dependent Claim
25 words
The composition of claim 11 wherein there are two stable coordinated compounds wherein the analyte portions are formed from leutinizing hormone and thyroid stimulating hormone.
Claim 14
Dependent Claim
26 words
The composition of claim 11 wherein there are two stable coordinated compounds wherein the analyte portions are formed from hepatitis B-surface antigen and hepatitis B-core antigen.
Claim 15
Dependent Claim
24 words
The composition of claim 11 wherein there are two stable coordinated compounds wherein the analyte portions are formed from thyroxine and thyroid stimulating hormone.
Claim 16
Dependent Claim
24 words
The composition of claim 11 wherein there are two stable coordinated compounds wherein the analyte portions are formed from thyroxine and thyroid binding globulin.
Claim 17
Dependent Claim
22 words
The composition of claim 11 wherein there are two stable coordinated compounds wherein the analyte portions are formed from angiotensin-II and renin.
Claim 18
Dependent Claim
23 words
The composition of claim 11 wherein there are two stable coordinated compounds wherein the analyte portions are formed from adrenocorticotrophic hormone and cortisol.
Claim 19
Dependent Claim
22 words
The composition of claim 11 wherein there are two stable coordinated compounds wherein the analyte portions are formed from insulin and C-peptide.
Claim 20
Dependent Claim
24 words
The composition of claim 11 wherein there are two stable coordinated compounds wherein the analyte portions are formed from estriol and human placental lactogen.
Claim 21
Dependent Claim
24 words
The composition of claim 1 wherein there are two stable coordinated compounds wherein the analyte portions are formed from lactate dehydrogenase and creatine phosphokinase.
Claim 22
Dependent Claim
27 words
The composition of claim 1 wherein there are two stable coordinated compounds wherein the analyte portions are formed from hepatitis B-surface antigen and human T-cell leukemia virus.
Claim 23
Dependent Claim
93 words
In a simultaneous multiple assay for two or more analytes, which assay includes the steps of: (a) contacting a sample and a mixture of radioisotope-labeled compounds in the presence of a mixture of binders for said analytes. (b) separating bound portions of the analytes from unbound portions of the analytes, and (c) counting radioactivity of at least one of the bound and unbound portions to determine the radioisotope-labeled compounds in the bound or unbound portions; the improvement comprising the condition that said mixture of radioisotope-labeled compounds is of the composition of claim 1.
Claim 24
Dependent Claim
93 words
In a simultaneous multiple assay for two or more analytes, which assay includes the steps of: (a) contacting a sample and a mixture of radioisotope-labeled compounds in the presence of a mixture of binders for said analytes, (b) separating bound portions of the analytes from unbound portions of the analytes, and (c) counting radioactivity of at least one of the bound and unbound portions to determine the radioisotope-labeled compounds in the bound or unbound portions; the improvement comprising the condition that said mixture of radioisotope-labeled compounds is of the composition of claim 2.
Claim 25
Dependent Claim
93 words
In a simultaneous multiple assay for two or more analytes, which assay includes the steps of: (a) contacting a sample and a mixture of radioisotope-labeled compounds in the presence of a mixture of binders for said analytes, (b) separating bound portions of the analytes from unbound portions of the analytes, and (c) counting radioactivity of at least one of the bound and unbound portions to determine the radioisotope-labeled compounds in the bound or unbound portions; the improvement comprising the condition that said mixture of radioisotope-labeled compounds is of the composition of claim 3.
Claim 26
Dependent Claim
93 words
In a simultaneous multiple assay for two or more analytes, which assay includes the steps of: (a) contacting a sample and a mixture of radioisotope-labeled compounds in the presence of a mixture of binders for said analytes, (b) separating bound portions of the analytes from unbound portions of the analytes, and (c) counting radioactivity of at least one of the bound and unbound portions to determine the radioisotope-labeled compounds in the bound or unbound portions; the improvement comprising the condition that said mixture of radiosotope-labeled compounds is of the composition of claim 4.
Claim 27
Dependent Claim
93 words
In a simultaneous multiple assay for two or more analytes, which assay includes the steps of: (a) contacting a sample and a mixture of radioisotope-labeled compounds in the presence of a mixture of binders for said analytes, (b) separating bound portions of the analytes from unbound portions of the analytes, and (c) counting radioactivity of at least one of the bound and unbound portions to determine the radioisotope-labeled compounds in the bound or unbound portions; the improvement comprising the condition that said mixture of radioisotope-labeled compounds is of the composition of claim 5.
Claim 28
Dependent Claim
93 words
In a simultaneous multiple assay for two or more analytes, which assay includes the steps of: (a) contacting a sample and a mixture of radioisotope-labeled compounds in the presence of a mixture of binders for said analytes, (b) separating bound portions of the analytes from unbound portions of the analytes, and (c) counting radioactivity of at least one of the bound and unbound portions to determine the radioisotope-labeled compounds in the bound or unbound portions; the improvement comprising the condition that said mixture of radioisotope-labeled compounds is of the composition of claim 6.
Claim 29
Dependent Claim
93 words
In a simultaneous multiple assay for two or more analytes, which assay includes the steps of: (a) contacting a sample and a mixture of radioisotope-labeled compounds in the presence of a mixture of binders for said analytes, (b) separating bound portions of the analytes from unbound portions of the analytes, and (c) counting radioactivity of at least one of the bound and unbound portions to determine the radioisotope-labeled compounds in the bound or unbound portions; the improvement comprising the condition that said mixture of radioisotope-labeled compounds is of the composition of claim 7.
Claim 30
Dependent Claim
93 words
In a simultaneous multiple assay for two or more analytes, which assay includes the steps of: (a) contacting a sample and a mixture of radioisotope-labeled compounds in the presence of a mixture of binders for said analytes, (b) separating bound portions of the analytes from unbound portions of the analytes, and (c) counting radioactivity of at least one of the bound and unbound portions to determine the radiosotope-labeled compounds in the bound or unbound portions; the improvement comprising the condition that said mixture of radioisotope-labeled compounds is of the composition of claim 8.
Claim 31
Dependent Claim
93 words
In a simultaneous multiple assay for two or more analytes, which assay includes the steps of: (a) contacting a sample and a mixture of radioisotope-labeled compounds in the presence of a mixture of binders for said analytes, (b) separating bound portions of the analytes from unbound portions of the analytes, and (c) counting radioactivity of at least one of the bound and unbound portions to determine the radioisotope-labeled compounds in the bound or unbound portions; the improvement comprising the condition that said mixture of radioisotope-labeled compounds is of the composition of claim 9.
Claim 32
Dependent Claim
93 words
In a simultaneous multiple assay for two or more analytes, which assay includes the steps of: (a) contacting a sample and a mixture of radioisotope-labeled compounds in the presence of a mixture of binders for said analytes, (b) separating bound portions of the analytes from unbound portions of the analytes, and (c) counting radioactivity of at least one of the bound and unbound portions to determine the radioisotope-labeled compounds in the bound or unbound portions; the improvement comprising the condition that said mixture of radioisotope-labeled compounds is of the composition of claim 10.
Claim 33
Dependent Claim
68 words
A kit useful in simultaneous multiple assay of one or more analytes comprising: a mixture of binders for said analytes; a mixture of radioisotope-labeled compounds, said mixture being of the composition of claim 1; means for contacting a sample with said mixture of rdioisotope-labeled compounds in the presence of said mixture of binders; and means for separating bound portions of the analytes from unbound portions of the analytes.
Claim 34
Dependent Claim
68 words
A kit useful in simultaneous multiple assay of one or more analytes comprising: a mixtures of binders for said analytes; a mixture of radiosotope-labeled compounds, said mixture being of the composition of claim 2; means for contacting a sample with said mixture of radioisotope-labeled compounds in the presence of said mixture of binders; and means for separating bound portions of the analytes from unbound portions of the analytes.
Claim 35
Dependent Claim
68 words
A kit useful in simultaneous multiple assay of one or more analytes comprising: a mixture of binders for said analytes; a mixture of radioisotope-labeled compounds, said mixture being of the composition of claim 3; means for contacting a sample with said mixture of radioisotope-labeled compounds in the presence of said mixture of binders; and means for separating bound portions of the analytes from unbound portions of the analytes.
Claim 36
Dependent Claim
68 words
A kit useful in simultaneous multiple assay of one or more analytes comprising: a mixture of binders for said analytes; a mixture of radioisotope-labeled compounds, said mixture being of the composition of claim 4; means for contacting a sample with said mixture of radioisotope-labeled compounds in the presence of said mixture of binders; and means for separating bound portions of the analytes from unbound portions of the analytes.
Claim 37
Dependent Claim
68 words
A kit useful in simultaneous multiple assay of one or more analytes comprising: a mixture of binders for said analytes; a mixture of radioisotope-labeled compounds, said mixture being of the composition of claim 5; means for contacting a sample with said mixture of radioisotope-labeled compounds in the presence of said mixture of binders; and means for separating bound portions of the analytes from unbound portions of the analytes.
Claim 38
Dependent Claim
68 words
A kit useful in simultaneous multiple assay of one or more analytes comprising: a mixture of binders for said analytes; a mixture of radioisotope-labeled compounds, said mixture being of the composition of claim 6; means for contacting a sample with said mixture of radioisotope-labeled compounds in the presence of said mixture of binders; and means for separating bound portions of the analytes from unbound portions of the analytes.
Claim 39
Dependent Claim
68 words
A kit useful in simultaneous multiple assay of one or more analytes comprising: a mixture of binders for said analytes; a mixture of radioisotope-labeled compounds, said mixture being of the composition of claim 7; means for contacting a sample with said mixture of radioisotope-labeled compounds in the presence of said mixture of binders; and means for separating bound portions of the analytes from unbound portions of the analytes.
Claim 40
Dependent Claim
68 words
A kit useful in simultaneous multiple assay of one or more analytes comprising: a mixture of binders for said analytes; a mixture of radioisotope-labeled compounds, said mixture being of the composition of claim 8; means for contacting a sample with said mixture of radioisotope-labeled compounds in the presence of said mixture of binders; and means for separating bound portions of the analytes from unbound portions of the analytes.
Claim 41
Dependent Claim
68 words
A kit useful in simultaneous multiple assay of one or more analytes comprising: a mixture of binders for said analytes; a mixture of radioisotopes-labeled compounds, said mixture being of the composition of claim 9; means for contacting a sample with said mixture of radioisotope-labeled compounds in the presence of said mixture of binders; and means for separating bound portions of the analytes from unbound portions of the analytes.
Claim 42
Dependent Claim
68 words
A kit useful in simultaneous multiple assay of one or more analytes comprising: a mixture of binders for said analytes; a mixture of radioisotope-labeled compounds, said mixture being of the composition of claim 10; means for contacting a sample with said mixture of radioisotope-labeled compounds in the presence of said mixture of binders; and means for separating bound portions of the analytes from unbound portions of the analytes.
Claim 43
Dependent Claim
19 words
A composition useful in simultaneous assay which comprises the composition of claim 1 and an analyte labeled with I-125.
Claim 44
Dependent Claim
19 words
A composition useful in simultaneous assay which comprises the composition of claim 2 and an analyte labeled with I-125.
Claim 45
Dependent Claim
19 words
A composition useful in simultaneous assay which comprises the composition of claim 3 and an analyte labeled with I-125.
Claim 46
Dependent Claim
19 words
A composition useful in simultaneous assay which comprises the composition of claim 4 and an analyte labeled with I-125.
Claim 47
Dependent Claim
19 words
A composition useful in simultaneous assay which comprises the composition of claim 5 and an analyte labeled with I-125.
Claim 48
Dependent Claim
24 words
A composition according to claim 46 wherein the coordinated compound analyte is leutinizing hormone and the analyte labeled with I-125 is follicle stimulating hormone.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-06-08 | 1990-12-08 | 1991-06-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-06-08 | 1994-12-08 | 1995-06-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-06-08 | 1998-12-08 | 1999-06-09 | $7700.00 | Window Closed / Lapsed |