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US4770994A
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Determination of carbohydrate acceptors
Assignee: Abbott Laboratories
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Filing: 1987-08-14
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Granted: 1988-09-13
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Expires: 2007-08-14
Abstract
Abstract A method for determining carbohydrate acceptors in a sample is disclosed wherein a sugar moiety is enzymatically transferred to the carbohydrate acceptor and the resulting carbohydrate acceptor-sugar complex is determined as a measure of the carbohydrate acceptor in the sample.
Inventors
- 👤 Harry G. Rittenhouse
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 07:29
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/5756; Y; Y10; Y10S; Y10S435/00; Y10S435/963
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
118 words
A method for determining elevated levels of incompletely glycosylated glycoprotein tumor markers in samples from cancer patients comprising; (a) intermixing with said sample containing said glycoprotein; (i) a sugar-nucleotide conjugate donor wherein the sugar moiety of said donor is capable of covalently labeling said glycoprotein; and (ii) an excess of exogenous enzyme capable of catalyzing the reaction of said glycoprotein and said donor; and (iii) a substrate diverter capable of preventing degradation of said donor, thereby enabling said sugar moiety to bind to said glycoprotein; (b) terminating the reaction of said glycoprotein and donor; then (c) determining the amount of incompletely glycosylated glycoprotein-sugar complex produced as a measure of the amount of said glycoprotein present in the sample.
Claim 2
Independent Claim
118 words
A method for determining elevated levels of incompletely glycosylated glycoprotein tumor markers in samples from cancer patients comprising; (a) intermixing with said sample containing said glycoprotein; (i) a sugar-nucleotide conjugate donor wherein the sugar moiety of said donor is capable of covalently labeling said glycoprotein; and (ii) an excess of exogenous enzyme capable of catalyzing the reaction of said glycoprotein and said donor; and (iii) a substrate diverter capable of preventing degradation of said donor, thereby enabling said sugar moiety to bind to said glycoprotein; (b) terminating the reaction of said glycoprotein and donor; then (c) determining the amount of incompletely glycosylated glycoprotein-sugar complex produced as a measure of the amount of said glycoprotein present in the sample.
Claim 3
Dependent Claim
16 words
A method according to claim 1 wherein a radio-labeled sugar-nucleotide conjugate is employed as the donor.
Claim 4
Dependent Claim
13 words
A method according to claim 4 wherein the sample is a serum sample.
Claim 5
Dependent Claim
14 words
A method according to claim 1 wherein a cofactor is added to the enzyme.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-09-13 | 1992-03-14 | 1992-09-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-09-13 | 1996-03-14 | 1996-09-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-09-13 | 2000-03-14 | 2000-09-13 | $7700.00 | Window Closed / Lapsed |