US4132600A
Expired / Lapsed
US
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Enzymatic noninvasive method for detecting cancer
Assignee: Massachusetts Institute of Technology
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Filing: 1977-06-01
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Granted: 1979-01-02
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Expires: 1997-06-01
Abstract
Abstract An enzymatic noninvasive method for detecting cancer in mammalian tissue is disclosed. This method comprises incubating exfoliated cells from mammalian tissue suspected of containing cancerous cells, and assaying them for galactosyl transferase activity. The level of galactosyl transferase activity has been found to be significantly altered in cells from cancerous tissue as compared to the level in cells from normal tissue.
Inventors
- 👤 George M. Plotkin
- 👤 George Wolf
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 04:13
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
24 words
A method for detecting cancer in mammalian tissue comprising assaying exfoliated cells from said tissue for a significantly altered level of galactosyl transferase activitiy.
Claim 2
Independent Claim
24 words
A method for detecting cancer in mammalian tissue comprising assaying exfoliated cells from said tissue for a significantly altered level of galactosyl transferase activitiy.
Claim 3
Dependent Claim
15 words
A method of claim 1 wherein said exfoliated cells are obtained noninvasively from body fluid.
Claim 4
Dependent Claim
62 words
An enzymatic, noninvasive test for cancer in the tissue of mammals, comprising: a. incubating broken cells exfoliated from said tissue with an exogenous galactose acceptor and an exogenous galactose-containing substrate; b. determining the level of galactose transferase activity present in said tissue; and, c. comparing the level of galactose transferase activity to the level associated with non-cancerous tissue of the same type.
Claim 5
Dependent Claim
62 words
An enzymatic, noninvasive test for cancer in the tissue of mammals, comprising: a. incubating broken cells exfoliated from said tissue with an exogenous galactose acceptor and an exogenous galactose-containing substrate; b. determining the level of galactose transferase activity present in said tissue; and, c. comparing the level of galactose transferase activity to the level associated with non-cancerous tissue of the same type.
Claim 6
Dependent Claim
13 words
The test of claim 3 wherein said exogenous galactose acceptor comprises a glycoprotein.
Claim 7
Dependent Claim
35 words
The test of claim 4 wherein said glycoprotein is selected from desialated ovine submaxillary mucin, desialated degalactosylated fetuin, desialated, bovine submaxillary mucin, and N-acetyl glucosamine and desialated ovine submaxillary mucin bound to an insoluble matrix.
Claim 8
Dependent Claim
18 words
The test of claim 5 wherein said exogenous galactose-containing substrate comprises a nucleotide or lipid bound to galactose.
Claim 9
Dependent Claim
14 words
The test of claim 5 wherein said exogenous galactose-containing substrate comprises uridine 5'-diphosphate galactose.
Claim 10
Dependent Claim
15 words
The test of claim 7 wherein said exogenous galactose-containing substrate comprises desialated ovine submaxillary mucin.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-01-01 | 1982-07-03 | 1983-01-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-01-01 | 1986-07-03 | 1987-01-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-01-01 | 1990-07-03 | 1991-01-02 | $7700.00 | Window Closed / Lapsed |