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US4769061A
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Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use
Assignee: CAPITOL BANK OF COMMERCE A CORP OF
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Filing: 1985-02-04
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Granted: 1988-09-06
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Expires: 2005-02-04
Abstract
Abstract Enhanced resistance to glyphosate, an inhibitor of the aromatic amino acid biosynthesis pathway, is imparted to a glyphosate sensitive host. A mutated aroA gene is employed which expresses 5-enolpyruvyl-3- phosphoshikimate synthase (EC: 2.5.1.19) (ES-3-P synthase). Methods are provided for obtaining the aroA mutation which provides the enzyme resistant to inhibition by glyphosate, means for introducing the structural gene into a sensitive host, as well as providing a method of producing the enzyme. The E. coli strain C600(pPMG1) has been deposited at the A.T.C.C. on Dec. 14, 1982 and given A.T.C.C. Accession No. 39256.
Inventors
- 👤 Luca Comai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 23:24
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/10; C12N9/1085; C12N9/1092; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8274; C12N15/8275; C12Q; C12Q1/00; C12Q1/48; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/581
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
47 words
A method for producing glyphosateresistant 5-enolpyruvyl3-phosphoshikimate synthase which comprises: growing a prokaryotic host containing a glyphosate-resistant 5-enolpyruvyl-3-phosphoshikimate synthase gene having at least one mutation originating from in vitro mutation in the aroA locus in an appropriate nutrient medium; and lysing said prokaryotic host and isolating said synthase.
Claim 2
Independent Claim
47 words
A method for producing glyphosateresistant 5-enolpyruvyl3-phosphoshikimate synthase which comprises: growing a prokaryotic host containing a glyphosate-resistant 5-enolpyruvyl-3-phosphoshikimate synthase gene having at least one mutation originating from in vitro mutation in the aroA locus in an appropriate nutrient medium; and lysing said prokaryotic host and isolating said synthase.
Claim 3
Dependent Claim
83 words
A plant entity consisting essentially of a plant cell, seed, or plant having a gene, as a result of the in vitro introduction of said gene into a plant cell, said gene encoding for a mutated glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthase enzyme, said gene being under the transcriptional control of regulatory signals functional in said plant entity, which said regulatory signals comprise plant derived transcriptional initiation regulatory signals, wherein when said plant entity is other than a cell, said plant entity is a dicotyledon.
Claim 4
Dependent Claim
83 words
A plant entity consisting essentially of a plant cell, seed, or plant having a gene, as a result of the in vitro introduction of said gene into a plant cell, said gene encoding for a mutated glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthase enzyme, said gene being under the transcriptional control of regulatory signals functional in said plant entity, which said regulatory signals comprise plant derived transcriptional initiation regulatory signals, wherein when said plant entity is other than a cell, said plant entity is a dicotyledon.
Claim 5
Dependent Claim
22 words
A plant cell, seed or plant according to claim 2, wherein said plant cell into which said gene is introduced is Brassica.
Claim 6
Dependent Claim
22 words
A plant cell, seed or plant according to claim 2, wherein said plant cell into which said gene is introduced is Nicotiana.
Claim 7
Dependent Claim
25 words
A plant cell, seed or plant according to claim 2, wherein said gene is heterologous to said plant cell in which said gene is introduced.
Claim 8
Dependent Claim
7 words
A plant seed according to claim 2.
Claim 9
Dependent Claim
36 words
A plant cell having a gene encoding for a mutated glyphosphate resistant 5-enopyruvyl-3-phosphoshikimate synthase enzyme, said gene being heterologous to said plant cell and under the transcriptional control of regulatory signals functional in said plant cell.
Claim 10
Dependent Claim
36 words
A plant cell having a gene encoding for a mutated glyphosphate resistant 5-enopyruvyl-3-phosphoshikimate synthase enzyme, said gene being heterologous to said plant cell and under the transcriptional control of regulatory signals functional in said plant cell.
Claim 11
Dependent Claim
17 words
A plant cell according to claim 7, wherein said regulatory signals comprise plant transcriptional initiation regulatory signals.
Claim 12
Independent Claim
63 words
A method for improving crop growth which comprises: applying to a field containing a dicotyledon crop having planted crop seeds or plants, which are glyphosate resistant due to having a mutated expressable gene encoding for glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthase enzyme originally resulting from introduction into cells of said plants, a sufficient amount of glyphosate to control weed growth without significantly affecting crop growth.
Claim 13
Independent Claim
63 words
A method for improving crop growth which comprises: applying to a field containing a dicotyledon crop having planted crop seeds or plants, which are glyphosate resistant due to having a mutated expressable gene encoding for glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthase enzyme originally resulting from introduction into cells of said plants, a sufficient amount of glyphosate to control weed growth without significantly affecting crop growth.
Claim 14
Dependent Claim
15 words
A method according to claim 9, wherein said gene is derived from a prokaryotic gene.
Claim 15
Dependent Claim
15 words
A method according to claim 9, wherein included with said glyphosate is a second herbicide.
Claim 16
Dependent Claim
22 words
A method according to claim 11, wherein said second herbicide is alachlor, metachlor, 2,4 D type, bentazon, bromoxynil, atrazine, cyanazine, or aciflurofen.
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