US4443971A
Expired / Lapsed
US
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Herbicide-tolerant plants
Assignee: Cornell Research Foundation Inc
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Filing: 1981-10-14
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Granted: 1984-04-24
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Expires: 2001-10-14
Abstract
Abstract This invention relates to herbicide-tolerant plants prepared by plant tissue culture. These plants are produced by culturing tissue of a plant sensitive to a particular herbicide in the presence of an amount of the herbicide, and for a time less than that sufficient to kill the entire plant-tissue population, but sufficient to kill a majority of the tissue population. If desired this procedure is repeated for sufficient passages, preferably with increasing amounts of said herbicide to provide a herbicide-tolerant strain of plant tissue which is subsequently subjected to differentiating growth conditions to provide herbicide-tolerant plants. In a preferred embodiment, plants, which display a heritable tolerance toward said herbicide as compared to the original plant, are selected from the whole population of tissue culture-derived plants. These plants can be propogated vegetatively using tissue culture methods to produce additional plants of the same genetic constitution. These plants also can be sexually reproduced to provide seeds and plants therefrom which display inherited tolerance to said herbicide.
Inventors
- 👤 Roy S. Chaleff
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 21:46
CPC Classifications
A; A01; A01H; A01H4/00; A01H4/002
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
126 words
A method for the preparation of a relatively herbicide tolerant plant, selected from the group consisting of tobacco, rice, corn, potato, oats, alfalfa, carrot and sugar cane, which displays heritable increased tolerance to said herbicide, which method comprises: (a) tissue culturing, in the presence of a herbicide to which a parent plant is sensitive, and to which a tissue culture derived from said parent plant is also sensitive, tissue from said parent plant, said tissue culturing being conducted in the presence of an amount of said herbicide sufficient to kill at least 90% of the tissue initially present and (b) subjecting the surviving culture tissue or plants derived therefrom to a process which isolates plant tissue or plants which display heritable increased tolerance to said herbicide.
Claim 2
Independent Claim
126 words
A method for the preparation of a relatively herbicide tolerant plant, selected from the group consisting of tobacco, rice, corn, potato, oats, alfalfa, carrot and sugar cane, which displays heritable increased tolerance to said herbicide, which method comprises: (a) tissue culturing, in the presence of a herbicide to which a parent plant is sensitive, and to which a tissue culture derived from said parent plant is also sensitive, tissue from said parent plant, said tissue culturing being conducted in the presence of an amount of said herbicide sufficient to kill at least 90% of the tissue initially present and (b) subjecting the surviving culture tissue or plants derived therefrom to a process which isolates plant tissue or plants which display heritable increased tolerance to said herbicide.
Claim 3
Dependent Claim
22 words
A method as in claim 1 wherein the amount of herbicide kills in excess of 99% of the plant tissue initially present.
Claim 4
Dependent Claim
28 words
A method as in claim 1 or 2 wherein the surviving cultured tissue is subjected to at least one additional culturing step in the presence of said herbicide.
Claim 5
Dependent Claim
23 words
A method as in claim 3 wherein each additional culturing step is conducted in the presence of an increased amount of said herbicide.
Claim 6
Independent Claim
140 words
A method for the preparation of a relatively herbicide tolerant plant, selected from the group consisting of tobacco, rice, corn, potato, oats, alfalfa, carrot and sugar cane, which displays heritable increased tolerance to said herbicide, which method comprises: (a) tissue culturing, in the presence of a herbicide, selected from the group consisting of picloram, paraquat, 2,4-D, glyphosphate, alachlor, atrizine and amitrole, to which a parent plant is sensitive, and to which a tissue culture derived from said parent plant is also sensitive, tissue from said parent plant, said tissue culturing being conducted in the presence of an amount of said herbicide sufficient to kill at least 90% of the tissue initially present and (b) subjecting the surviving culture tissue or plants derived therefrom to a process which isolates plant tissue or plants which display heritable increased tolerance to said herbicide.
Claim 7
Independent Claim
140 words
A method for the preparation of a relatively herbicide tolerant plant, selected from the group consisting of tobacco, rice, corn, potato, oats, alfalfa, carrot and sugar cane, which displays heritable increased tolerance to said herbicide, which method comprises: (a) tissue culturing, in the presence of a herbicide, selected from the group consisting of picloram, paraquat, 2,4-D, glyphosphate, alachlor, atrizine and amitrole, to which a parent plant is sensitive, and to which a tissue culture derived from said parent plant is also sensitive, tissue from said parent plant, said tissue culturing being conducted in the presence of an amount of said herbicide sufficient to kill at least 90% of the tissue initially present and (b) subjecting the surviving culture tissue or plants derived therefrom to a process which isolates plant tissue or plants which display heritable increased tolerance to said herbicide.
Claim 8
Dependent Claim
22 words
A method as in claim 5 wherein the amount of herbicide kills in excess of 99% of the plant tissue initially present.
Claim 9
Dependent Claim
28 words
A method as in claim 5 or 6 wherein the surviving cultured tissue is subjected to at least one additional culturing step in the presence of said herbicide.
Claim 10
Dependent Claim
23 words
A method as in claim 7 wherein each additional culturing step is conducted in the presence of an increased amount of said herbicide.
Claim 11
Dependent Claim
11 words
A method as in claim 1 where the plant is tobacco.
Claim 12
Dependent Claim
11 words
A method as in claim 5 where the plant is tobacco.
Claim 13
Independent Claim
19 words
A method as in claims 1, 5 or 10 where the culturing step is a suspension tissue culturing step.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-04-24 | 1987-10-24 | 1988-04-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-04-24 | 1991-10-24 | 1992-04-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-04-24 | 1995-10-24 | 1996-04-24 | $7700.00 | Window Closed / Lapsed |