US4766203A
Expired / Lapsed
US
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New strain of Bacillus, a toxin derived thereof and a composition for combating Coleoptera
Assignee: ENTOTECH Inc A DE CORP
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Filing: 1987-03-13
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Granted: 1988-08-23
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Expires: 2007-03-13
Abstract
Abstract The present invention provides a new strain of micro-organism, Bacillus thuringiensis of pathotype C, a process for obtaining a toxin therefrom and an insecticide for combating Coleoptera containing the new micro-organism or a toxin obtained therefrom.
Inventors
- 👤 Aloisius Krieg
- 👤 Alois M. Huger
- 👤 Wolfgang Schnetter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 00:19
CPC Classifications
A; A01; A01N; A01N63/00; A01N63/50; C; C07; C07K; C07K14/00; C07K14/195; C07K14/32; C07K14/325; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/07; C12R2001/075; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S530/00; Y10S530/82; Y10S530/825
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
77 words
A toxin against Coleoptera isolated from a strain of Bacillus thuringiensis characterized in that said toxin is a protein with a molecular weight of from 65 to 70 kilodaltons, is obtained from parasporal crystals produced by said Bacillus thuringiensis, said crystals characterized by a flat, plate-like form with an edge length of from about 0.8 to 1.5 μm, said toxin further characterized by producing antibodies which do not cross react with other toxins obtained from Bacillus thuringiensis.
Claim 2
Dependent Claim
77 words
A toxin against Coleoptera isolated from a strain of Bacillus thuringiensis characterized in that said toxin is a protein with a molecular weight of from 65 to 70 kilodaltons, is obtained from parasporal crystals produced by said Bacillus thuringiensis, said crystals characterized by a flat, plate-like form with an edge length of from about 0.8 to 1.5 μm, said toxin further characterized by producing antibodies which do not cross react with other toxins obtained from Bacillus thuringiensis.
Claim 3
Dependent Claim
59 words
A process for obtaining a toxin against Coleoptera, said toxin characterized as being a protein with molecular weight of from 65 to 70 kilodaltons comprising lysing sporangia of Bacillus thuringiensis which contain crystals comprising said toxin, separating crystalline mass from the spores and cell residues of said Bacillus thuringiensis, and purifying the crystalline mass to obtain said toxin therefrom.
Claim 4
Dependent Claim
59 words
A process for obtaining a toxin against Coleoptera, said toxin characterized as being a protein with molecular weight of from 65 to 70 kilodaltons comprising lysing sporangia of Bacillus thuringiensis which contain crystals comprising said toxin, separating crystalline mass from the spores and cell residues of said Bacillus thuringiensis, and purifying the crystalline mass to obtain said toxin therefrom.
Claim 5
Dependent Claim
81 words
An insecticide for combating Coleoptera comprising Bacillus thuringiensis which produce Coleoptera specific toxin wherein said toxin is a protein molecule of 65 to 70 kilodaltons obtained from parasporal crystals produced by said Bacillus thuringiensis, said crystals characterized by a flat, plate-like form with an edge length of from about 0.8 to 1.5 μm, said toxin further characterized by producing antibodies which do not cross react with other toxins obtained from Bacillus thuringiensis or the toxin derived therefrom and a suitable carrier.
Claim 6
Dependent Claim
81 words
An insecticide for combating Coleoptera comprising Bacillus thuringiensis which produce Coleoptera specific toxin wherein said toxin is a protein molecule of 65 to 70 kilodaltons obtained from parasporal crystals produced by said Bacillus thuringiensis, said crystals characterized by a flat, plate-like form with an edge length of from about 0.8 to 1.5 μm, said toxin further characterized by producing antibodies which do not cross react with other toxins obtained from Bacillus thuringiensis or the toxin derived therefrom and a suitable carrier.
Claim 7
Dependent Claim
21 words
A method of combating Colorado beetles in host plants comprising, applying an insecticidally effective amount of the insecticide of claim 3.
Claim 8
Dependent Claim
28 words
The method of claim 4 wherein 10 12 to 10 15 spores and an equivalent amount of toxic crystals of said inseoticide are applied per hectacre of plants.
Claim 9
Dependent Claim
23 words
A method of claim 5 wherein 10 13 to 10 14 spores and an equivalent number of toxic crystals per hectacre are used.
Claim 10
Dependent Claim
13 words
The method of claim 6 wherein the host plant is a potato plant.
Claim 11
Dependent Claim
85 words
An insecticide for combating Coleoptera comprising Bacillus thuringiensis var. tenebrionis DSM 2803 which produces Coleoptera specific toxin, wherein said toxin is a protein molecule of 65 to 70 kilodaltons obtained from parasporal crystals produced by said Bacillus thuringiensis, said crystals characterized by a flat, plate-like form with an edge length of from about 0.8 to 1.5 μm, said toxin further characterized by producing antibodies which do not cross react with other toxins obtained from Bacillus thuringiensis, or the toxin derived therefrom, and a suitable carrier.
Claim 12
Dependent Claim
85 words
An insecticide for combating Coleoptera comprising Bacillus thuringiensis var. tenebrionis DSM 2803 which produces Coleoptera specific toxin, wherein said toxin is a protein molecule of 65 to 70 kilodaltons obtained from parasporal crystals produced by said Bacillus thuringiensis, said crystals characterized by a flat, plate-like form with an edge length of from about 0.8 to 1.5 μm, said toxin further characterized by producing antibodies which do not cross react with other toxins obtained from Bacillus thuringiensis, or the toxin derived therefrom, and a suitable carrier.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-08-23 | 1992-02-22 | 1992-08-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-23 | 1996-02-22 | 1996-08-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-23 | 2000-02-22 | 2000-08-23 | $7700.00 | Window Closed / Lapsed |