US4133716A
Expired / Lapsed
US
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Method for the biosynthesis of a microbial insecticide
Assignee: CRC RI RICERCA CHIMICA SpA VIA PESENALAT 6 - S GIOVANNI AL NATISONE (UDINE) (ITALY) A CORP OF ITALY Cia
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Filing: 1976-11-22
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Granted: 1979-01-09
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Expires: 1996-11-22
Abstract
Abstract A method for the biosynthesis of a microbial insecticide containing spores and crystalline endotoxin, in which a Bacillus thuringiensis microorganism is cultivated in a nutrient medium under formation of spores in such a way that the early lysis of the cells caused in the course of the submerged cultivation by the ventilation prior to the end of the formation of spores is prevented, is described.
Inventors
- 👤 Branimir Zamola
- 👤 Franjo Kajfez
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 04:15
CPC Classifications
C; C12; C12N; C12N3/00; A; A01; A01N; A01N63/00; A01N63/20; A01N63/22; A01N63/23; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
59 words
A method for the biosynthesis of a microbial insecticide containing spores and crystalline endotoxin, which comprises cultivating, by submerged cultivation, a Bacillus thuringiensis microoganism in a nutrient medium under formation of spores and preventing, by anaerobic shock, early lysis of the cells resulting during the course of the submerged cultivation by ventilation prior to the completion of spore formation.
Claim 2
Independent Claim
59 words
A method for the biosynthesis of a microbial insecticide containing spores and crystalline endotoxin, which comprises cultivating, by submerged cultivation, a Bacillus thuringiensis microoganism in a nutrient medium under formation of spores and preventing, by anaerobic shock, early lysis of the cells resulting during the course of the submerged cultivation by ventilation prior to the completion of spore formation.
Claim 3
Dependent Claim
49 words
A method according to claim 1, wherein the Bacillus thuringiensis is cultivated without lysis prior to the end of the formation of spores by interrupting the ventilation for 3 to 6 hours in the twelfth hour of cultivation and carrying out the cultivation under anaerobic conditions during that time.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-01-08 | 1982-07-10 | 1983-01-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-01-08 | 1986-07-10 | 1987-01-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-01-08 | 1990-07-10 | 1991-01-09 | $7700.00 | Window Closed / Lapsed |