US4762915A
Expired / Lapsed
US
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Protein-liposome conjugates
Assignee: LIPSOME TECHNOLOGY Inc
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Filing: 1985-01-18
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Granted: 1988-08-09
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Expires: 2005-01-18
Abstract
Abstract A lipid coupling reagent for use in coupling amine-containing molecules, such as proteins, to liposomes. The reagent includes phosphatidylethanolamine coupled to a 3-20 atom carbon-containing spacer arm terminating at a carboxyl or thiocarboxyl group. Also disclosed are liposomes prepared to include between about 1 and 20 mole percent of the coupling reagent, and liposomes containing surface arrays of proteins attached to the liposomes through the coupling reagent.
Inventors
- 👤 Viola T. Kung
- 👤 Carl T. Redemann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 23:54
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/585; G01N33/586; A; A61; A61K; A61K47/00; A61K47/50; A61K47/69; A61K47/6905; A61K47/6911; A61K47/6913; A61K9/00; A61K9/10; A61K9/127; Y; Y10; Y10S; Y10S424/00; Y10S424/812
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
101 words
A method of attaching protein molecules to liposomes at a concentration of at least about 150 μg protein per μmole lipid, comprising providing a carboxylated lipid coupling reagent of the form ##STR11## where PE--NH 2 is phosphatidylethanolamine and CH 2 - - - CH 2 is a carbon-containing chain having a total chain length between 3 and about 20 atoms preparing liposomes containing between about 1 and 20 mole percent of the coupling reagent, activating the liposomes with a water-soluble carbodiimide, and reacting the activated liposomes with such protein, at a protein concentration of at least about 300-500 μg/μmole liposome lipid.
Claim 2
Independent Claim
101 words
A method of attaching protein molecules to liposomes at a concentration of at least about 150 μg protein per μmole lipid, comprising providing a carboxylated lipid coupling reagent of the form ##STR11## where PE--NH 2 is phosphatidylethanolamine and CH 2 - - - CH 2 is a carbon-containing chain having a total chain length between 3 and about 20 atoms preparing liposomes containing between about 1 and 20 mole percent of the coupling reagent, activating the liposomes with a water-soluble carbodiimide, and reacting the activated liposomes with such protein, at a protein concentration of at least about 300-500 μg/μmole liposome lipid.
Claim 3
Dependent Claim
24 words
The method of claim 1, wherein CH 2 - - - CH 2 is a hydrocarbon chain between about 8 and 12 carbons long.
References Cited (Backward Citations)
US4485093A
Cited
US4486344A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-08-09 | 1992-02-08 | 1992-08-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-09 | 1996-02-08 | 1996-08-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-09 | 2000-02-08 | 2000-08-09 | $7700.00 | Window Closed / Lapsed |