US4486344A
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Urea-linked immunogens, antibodies, and preparative method
Assignee: Bayer Corp
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Filing: 1983-03-28
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Granted: 1984-12-04
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Expires: 2003-03-28
Abstract
Abstract Immunogen conjugates comprising amino-functionalized haptens coupled through a carbonyl bridge to amino groups in immunogenic proteins and polypeptides. The resulting simple urea linkage in the conjugate is hydrophilic and contributes essentially no haptenic determinants. The immunogen is characterized by a high epitopic density. The method involves reaction of the hapten with a carbonyl diimidazole followed by addition of the protein or polypeptide carrier. Protein crosslinking problems common to prior art coupling methods are avoided.
Inventors
- 👤 Robert T. Buckler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 00:25
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/808; Y10S530/809
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
82 words
An immunogen conjugate for use in preparing antibodies against a particular hapten which contains a primary or secondary amine group, which conjugate is of the formula: ##STR2## wherein hapten(amino)--represents said hapten coupled through said primary or secondary amine group, --(NH)Carrier represents an immunogenic protein or polypeptide coupled directly through amino groups thereon, whereby said amine-containing hapten is coupled to said Carrier through a urea linkage, and p is on the average from 1 to the number of available amino groups on Carrier.
Claim 2
Dependent Claim
82 words
An immunogen conjugate for use in preparing antibodies against a particular hapten which contains a primary or secondary amine group, which conjugate is of the formula: ##STR2## wherein hapten(amino)--represents said hapten coupled through said primary or secondary amine group, --(NH)Carrier represents an immunogenic protein or polypeptide coupled directly through amino groups thereon, whereby said amine-containing hapten is coupled to said Carrier through a urea linkage, and p is on the average from 1 to the number of available amino groups on Carrier.
Claim 3
Dependent Claim
16 words
The conjugate of claim 1 wherein p is on the average from 1 to about 50.
Claim 4
Dependent Claim
14 words
The conjugate of claim 1 wherein said immunogenic protein or polypeptide is an albumin.
Claim 5
Dependent Claim
18 words
The conjugate of claim 1 wherein said hapten has a molecular weight between about 100 and about 1500.
Claim 6
Dependent Claim
10 words
An antibody prepared against the immunogen conjugate of claim 1.
Claim 7
Dependent Claim
10 words
An antibody prepared against the immunogen conjugate of claim 2.
Claim 8
Dependent Claim
10 words
An antibody prepared against the immunogen conjugate of claim 4.
Claim 9
Dependent Claim
35 words
A method for preparing an immunogen conjugate according to claim 1, comprising the steps of reacting said hapten with a carbonyl diimidazole and thereafter contacting the resulting activated hapten with said immunogenic protein or polypeptide.
Claim 10
Dependent Claim
32 words
The conjugate of claim 1 wherein said hapten contains a primary amine group and wherein said conjugate has the formula: ##STR3## wherein hapten (NH)--represents said hapten coupled through said primary amine group.
References Cited (Backward Citations)
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