Patent US4755460A successfully refreshed from Google Patents.
US4755460A
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Bovine antigen glycoprotein, related antibody, and use in detection of pregnancy in cattle
Assignee: University of Minnesota Twin Cities
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Filing: 1984-07-06
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Granted: 1988-07-05
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Expires: 2004-07-06
Abstract
Abstract A bovine pregnancy antigen, determined to be a glycoprotein, has been isolated and purified. If is diagnostic for the presence of pregnancy in cattle when detected by the use of antibodies to the antigen.
Inventors
- 👤 Eileen F. Bostwick
- 👤 Alan G. Hunter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 07:30
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/689; A; A61; A61K; A61K39/00; A61K39/0005; G01N2800/00; G01N2800/36; G01N2800/368; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S436/00; Y10S436/811; Y10S436/814; Y10S436/828; Y10S530/00; Y10S530/827; Y10S530/85
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
103 words
An isolated, substantially pure bovine pregnancy antigen for detecting and determining pregnancy in cattle consisting of a glycoprotein obtained from a pregnant bovine animal which (a) binds nicotinamide-adenine dinucleotide, (b) has immunoreactive portions with a molecular weight of about 158,000 to 263,000, (c) contains at least the carbohydrates N-Ac-glucosamine, galactose, and L-fucose, and either D-mannose or D-glucose or both, (d) has an isoelectric point range of 4.5 to 5.5, (e) gives a blue stain with Coomassie Blue, and (f) is not cross-reactive when antibodies directed to said glycoprotein are contacted with hemoglobin, albumin, bovine luteinizing hormone, IgG, fibrinogen, fetuin, or alpha fetal protein.
Claim 2
Dependent Claim
103 words
An isolated, substantially pure bovine pregnancy antigen for detecting and determining pregnancy in cattle consisting of a glycoprotein obtained from a pregnant bovine animal which (a) binds nicotinamide-adenine dinucleotide, (b) has immunoreactive portions with a molecular weight of about 158,000 to 263,000, (c) contains at least the carbohydrates N-Ac-glucosamine, galactose, and L-fucose, and either D-mannose or D-glucose or both, (d) has an isoelectric point range of 4.5 to 5.5, (e) gives a blue stain with Coomassie Blue, and (f) is not cross-reactive when antibodies directed to said glycoprotein are contacted with hemoglobin, albumin, bovine luteinizing hormone, IgG, fibrinogen, fetuin, or alpha fetal protein.
Claim 3
Dependent Claim
60 words
A method for detecting the presence of a bovine pregnancy antigen in the blood serum, urine, milk, saliva, or tissue extract of a female bovine which comprises, contacting a sample of the blood serum, urine, milk or tissue extract to be tested with antiserum to the bovine pregnancy antigen of claim 1 and examining the sample for evidence of immunoreactivity.
Claim 4
Dependent Claim
38 words
A monoclonal antibody produced by a hybridoma formed by fusion of cells from a mouse myeloma line and spleen cells from a mouse previously immunized with the bovine pregnancy antigen of claim 1, with which said antibody reacts.
Claim 5
Dependent Claim
32 words
A test kit, to be used for the detection and determination of pregnancy in cattle comprising (a) the monoclonal antibody of claim 3, (b) peroxidase-labeled protein A, and (c) color changing substrate.
Claim 6
Dependent Claim
44 words
A method for the early detection of pregnancy in bovine animals comprising contacting a serum sample of a female bovine animal with the components of a test kit of claim 4 wherein a color change positively indicates the pregnancy of said female bovine animal.
Claim 7
Dependent Claim
45 words
A method for the early detection of pregnancy in bovine animals comprising contacting a serum sample of a female bovine animal with the bovine pregnancy antigen of claim 1 wherein the subsequent presence of antibody-antigen complexes positively indicate the pregnancy of said female bovine animal.
Claim 8
Dependent Claim
14 words
The method of claim 6 wherein bovine pregnancy is detected within one estrus cycle.
Claim 9
Dependent Claim
14 words
The method of claim 6 wherein bovine pregnancy is detected 12-15 days after insemination.
Claim 10
Dependent Claim
14 words
The method of claim 6 wherein bovine pregnancy is detected 13 days after insemination.
Claim 11
Dependent Claim
89 words
A process for isolating a bovine pregnancy antigen comprising (a) combining chorion or chorionic fluid from pregnant cows with an inorganic physiologically compatible salt whereby a first precipitate is formed, (b) resuspending and desalting said first precipitate to form a concentrated fluid preparation, (c) filtering said concentrated fluid preparation on an anion-exchanger whereby a bovine pregnancy antigen is adsorbed on said anion-exchanger, (d) eluting said antigen from said anion-exchanger to form a crude glycoprotein of about 400,000 molecular weight, and (e) recovering said antigen of about 400,000 molecular weight.
Claim 12
Dependent Claim
89 words
A process for isolating a bovine pregnancy antigen comprising (a) combining chorion or chorionic fluid from pregnant cows with an inorganic physiologically compatible salt whereby a first precipitate is formed, (b) resuspending and desalting said first precipitate to form a concentrated fluid preparation, (c) filtering said concentrated fluid preparation on an anion-exchanger whereby a bovine pregnancy antigen is adsorbed on said anion-exchanger, (d) eluting said antigen from said anion-exchanger to form a crude glycoprotein of about 400,000 molecular weight, and (e) recovering said antigen of about 400,000 molecular weight.
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