Patent US4743561A successfully refreshed from Google Patents.
US4743561A
Expired / Lapsed
US
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Luminescent assay with a reagent to alter transmitive properties of assay solution
Assignee: Abbott Laboratories
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Filing: 1985-03-05
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Granted: 1988-05-10
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Expires: 2005-03-05
Abstract
Abstract An improved method and reagents are disclosed for determining a ligand in an assay solution containing the ligand, a reagent system and a luminescent compounds, wherein the intensity of the light emitted by the assay solution is related to the change in the transmittive properties of the assay solution produced by the interaction of the ligand to be determined and a reagent system capable of producing a change in the transmittive properties of the assay solution in the presence of the ligand.
Inventors
- 👤 Mark R. Shaffar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 16:17
CPC Classifications
G; G01; G01L; G01L3/00; C; C12; C12Q; C12Q1/00; C12Q1/54; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/909; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444; Y10T436/145555; Y10T436/146666; Y10T436/147777; Y10T436/16; Y10T436/166666; Y10T436/17; Y10T436/171538; Y10T436/19; Y10T436/20; Y10T436/203332; Y10T436/204165
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
107 words
A method for determining a ligand in a sample suspected of containing the ligand, which method comprises: (a) combining to form an assay solution (i) said sample; (ii) a luminescent reagent; (iii) a reagent system which in the presence of the ligand to be determined is capable of providing a change in the transmittive properties of the assay solution within a wavelength band that overlaps the emission wavelength band of light produced by the luminescent reagent; (b) activating the luminescent reagent; and (c) measuring the intensity of light emitted by the assay solution as a measure of the presence or amount of the ligand in the sample.
Claim 2
Independent Claim
107 words
A method for determining a ligand in a sample suspected of containing the ligand, which method comprises: (a) combining to form an assay solution (i) said sample; (ii) a luminescent reagent; (iii) a reagent system which in the presence of the ligand to be determined is capable of providing a change in the transmittive properties of the assay solution within a wavelength band that overlaps the emission wavelength band of light produced by the luminescent reagent; (b) activating the luminescent reagent; and (c) measuring the intensity of light emitted by the assay solution as a measure of the presence or amount of the ligand in the sample.
Claim 3
Dependent Claim
20 words
A method according to claim 1 wherein the reagent system is a chromogenic reagent system or a turbidimetric reagent system.
Claim 4
Dependent Claim
15 words
A method according to claim 2 wherein the reagent system is a chromogenic reagent system.
Claim 5
Dependent Claim
12 words
A method according to claim 1 wherein the luminescent reagent is chemiluminescent.
Claim 6
Dependent Claim
12 words
A method according to claim 1 wherein the luminescent reagent is bioluminescent.
Claim 7
Dependent Claim
69 words
A reagent composition useful for determining the presence or amount of a ligand in a sample suspected of containing said ligand, said composition comprising a reagent system capable of providing a change in the transmittive properties of a solution containing said ligand, and a luminescent reagent having an emission wavelength within the wavelength band associated with the change in transmittive properties of a solution containing said reagent and ligand.
Claim 8
Dependent Claim
69 words
A reagent composition useful for determining the presence or amount of a ligand in a sample suspected of containing said ligand, said composition comprising a reagent system capable of providing a change in the transmittive properties of a solution containing said ligand, and a luminescent reagent having an emission wavelength within the wavelength band associated with the change in transmittive properties of a solution containing said reagent and ligand.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-05-10 | 1991-11-09 | 1992-05-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-10 | 1995-11-09 | 1996-05-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-10 | 1999-11-09 | 2000-05-10 | $7700.00 | Window Closed / Lapsed |