US4743560A
Expired / Lapsed
US
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Solid phase assay
Assignee: Becton Dickinson and Co
•
Filing: 1984-03-26
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Granted: 1988-05-10
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Expires: 2004-03-26
Abstract
Abstract Solid phase assay for an analyte wherein binder is supported on a solid support, such as nitrocellulose, and the tracer is comprised of ligand labeled with a particulate label, such as a liposome, including a detectable marker which is not visible.
Inventors
- 👤 Robert L. Campbell
- 👤 Daniel B. Wagner
- 👤 James P. O'Connell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 11:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/58; G01N33/585; G01N33/586; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/807; Y10S436/808; Y10S436/815; Y10S436/817; Y10S436/818; Y10S436/829
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
141 words
A process for assaying for an analyte, comprising: contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a specific binder for at least the analyte, said tracer being comprised of a ligand labeled with a liposome, said liposome including a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support, said test area being formed of a material having a surface area for supporting the binder and said binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome; and determining the tracer bound in said test area as a measure of analyte in a sample.
Claim 2
Dependent Claim
141 words
A process for assaying for an analyte, comprising: contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a specific binder for at least the analyte, said tracer being comprised of a ligand labeled with a liposome, said liposome including a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support, said test area being formed of a material having a surface area for supporting the binder and said binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome; and determining the tracer bound in said test area as a measure of analyte in a sample.
Claim 3
Dependent Claim
19 words
The process of claim 1 wherein analyte and tracer are sequentially contacted with the binder on the solid support.
Claim 4
Dependent Claim
19 words
The process of claim 1 wherein the analyte and tracer are simultaneously contacted with binder on the solid support.
Claim 5
Dependent Claim
10 words
The process of claim 1 wherein the analyte is digoxin.
Claim 6
Dependent Claim
13 words
The process of claim 1 wherein the analyte is (hCG) human chorionic gonadotropin.
Claim 7
Dependent Claim
15 words
The process of claim 1 wherein the tracer is determined without lysis of the liposome.
Claim 8
Dependent Claim
13 words
The process of claim 1 wherein the solid support is in sheet form.
Claim 9
Dependent Claim
11 words
The process of claim 1 wherein the binder is an antibody.
Claim 10
Dependent Claim
15 words
The process of claim 8 wherein the tracer is a labeled form of the analyte.
Claim 11
Dependent Claim
18 words
The process of claim 8 wherein the tracer is a labeled form of an antibody for the analyte.
Claim 12
Dependent Claim
45 words
The process of claim 1 wherein the assay is for human chorionic gonadotropin the binder is antibody to human chorionic gonadotropin and said tracer is comprised of antibody to human chorionic gonadotropin labeled with a (sac) liposome containing a fluorescent material as said detectable marker.
Claim 13
Dependent Claim
13 words
The process of claim 11 wherein the test area is formed of nitrocellulose.
Claim 14
Dependent Claim
50 words
The process of claim 1 wherein the assay is for digoxin, said binder is antibody to digoxin and the tracer is comprised of an analogue of digoxin (bound by said antibody) labeled with a sac containing a fluorescent material as said detectable marker(.), said analogue being bound by said antibody.
Claim 15
Dependent Claim
13 words
The process of claim 13 wherein the test area is formed of nitrocellulose.
Claim 16
Dependent Claim
129 words
A process for assaying for an analyte, comprising: contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a binder specific for at least the analyte, said tracer being comprised of a ligand labled with a liposome including a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support, said test area being formed of nitrocellulose, said binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome; and determining the tracer bound in said test area as a measure of analyte in a sample.
Claim 17
Dependent Claim
129 words
A process for assaying for an analyte, comprising: contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a binder specific for at least the analyte, said tracer being comprised of a ligand labled with a liposome including a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support, said test area being formed of nitrocellulose, said binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome; and determining the tracer bound in said test area as a measure of analyte in a sample.
Claim 18
Dependent Claim
19 words
The process of claim 15 wherein the tracer in said test area is determined without lysis of the liposome.
Claim 19
Dependent Claim
13 words
The process of claim 16 wherein the analyte is (hCG) human chorionic gonadotropin.
Claim 20
Dependent Claim
10 words
The process of claim 16 wherein the analyte is digoxin.
Claim 21
Dependent Claim
22 words
The process of claim 16 wherein the analyte is in a sample in a concentration of no greater than 10 -9 gm/ml
Claim 22
Dependent Claim
22 words
The process of claim 15 wherein the analyte is in a sample in a concentration of no greater than 10 -9 gm/ml.
Claim 23
Dependent Claim
13 words
The process of claim 15 wherein the solid support is in sheet form.
Claim 24
Dependent Claim
15 words
The process of claim 15 wherein the support contains a plurality of said test areas.
Claim 25
Dependent Claim
20 words
The process of claim 15 wherein the binder is supported in a concentration of at least 40 μg/cm 2 .
Claim 26
Dependent Claim
25 words
The process of claim 23 wherein the binder is in the test area as a spot having a diameter of from 3 to 5 mm.
Claim 27
Dependent Claim
120 words
A reagent kit for determining an analyte comprising: a solid support including a binder on a test area of a solid support; and a tracer, said binder being a binder specific for at least the analyte, said tracer being comprised of a ligand labeled with a liposome containing a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support during an assay, said test area being formed of a material having a surface area supporting the binder and the binder being supported in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome.
Claim 28
Dependent Claim
120 words
A reagent kit for determining an analyte comprising: a solid support including a binder on a test area of a solid support; and a tracer, said binder being a binder specific for at least the analyte, said tracer being comprised of a ligand labeled with a liposome containing a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support during an assay, said test area being formed of a material having a surface area supporting the binder and the binder being supported in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome.
Claim 29
Dependent Claim
14 words
The kit of claim 25 wherein the (binder) test area is formed of nitrocellulose.
Claim 30
Dependent Claim
21 words
The kit of claim 26 wherein the label of the tracer is a liposome including a fluorescent material as said marker.
Claim 31
Dependent Claim
11 words
The kit of claim 26 wherein the binder is an antibody.
Claim 32
Dependent Claim
27 words
The kit of claim 28 wherein the analyte is digoxin, the binder is antibody to digoxin and the ligand of the tracer is an analogue of digoxin.
Claim 33
Dependent Claim
13 words
The kit of claim 28 wherein the analyte is (hCG) human chorionic gonadotropin.
Claim 34
Dependent Claim
28 words
The kit of claim 30 wherein the binder is antibody to (hCG) human chorionic gonadotropin and the ligand of the tracer is antibody to (hCG) human chorionic gonadotropin.
Claim 35
Dependent Claim
119 words
A process for assaying for an analyte, comprising: contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a specific binder for at least the analyte, said tracer being comprised of a ligand labeled with a lipsome, said liposome including a detectable marker which is not visible, said test area being formed of a material having a surface area for supporting the binder and the binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome; and determining the tracer bound in said test area as a measure of analyte in a sample.
Claim 36
Dependent Claim
119 words
A process for assaying for an analyte, comprising: contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a specific binder for at least the analyte, said tracer being comprised of a ligand labeled with a lipsome, said liposome including a detectable marker which is not visible, said test area being formed of a material having a surface area for supporting the binder and the binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome; and determining the tracer bound in said test area as a measure of analyte in a sample.
Claim 37
Dependent Claim
13 words
The process of claim 32 wherein the test area is formed of nitrocellulose.
Claim 38
Dependent Claim
15 words
The process of claim 32, wherein the tracer is determined without lysis of the liposome.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-05-10 | 1991-11-09 | 1992-05-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-10 | 1995-11-09 | 1996-05-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-10 | 1999-11-09 | 2000-05-10 | $7700.00 | Window Closed / Lapsed |