US4724210A
Expired / Lapsed
US
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Method for purification of influenza virus
Assignee: JURIDACAL FOUNDATION CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE 668 OKUBO SHIMIZU-MACHI KUMAMOTO-SHI KUMAMOTO-KEN JAPAN
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Filing: 1985-08-09
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Granted: 1988-02-09
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Expires: 2005-08-09
Abstract
Abstract Disclosed is a method for the purification of an influenza virus, which comprises subjecting a solution containing the influenza virus to column chromatography using, as a gel for chromatography, a sulfuric acid ester of cellulose or a crosslinked polysaccharide. The method can provide highly purified influenza virus which is useful for obtaining an effective vaccine against influenza.
Inventors
- 👤 Tetsuya Oka
- 👤 Kunio Ohkuma
- 👤 Tetsuo Kawahara
- 👤 Mitsuo Sakoh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 12:42
CPC Classifications
C; C12; C12N; C12N7/00; A; A61; A61K; A61K39/00; A61K39/12; A61K39/145; C12N2760/00; C12N2760/00011; C12N2760/16011; C12N2760/16111; C12N2760/16134; C12N2760/16151
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
60 words
A method for the purification of an influenza virus, which comprises subjecting a solution containing the influenza virus to column chromatography using, as a gel for chromatography, a sulfuric acid ester of cellulose or a crosslinked polysaccharide, said sulfuric acid ester being prepared by treating a gel of cellulose or crosslinked polysaccharide with a sulfating agent in an organic solvent.
Claim 2
Independent Claim
60 words
A method for the purification of an influenza virus, which comprises subjecting a solution containing the influenza virus to column chromatography using, as a gel for chromatography, a sulfuric acid ester of cellulose or a crosslinked polysaccharide, said sulfuric acid ester being prepared by treating a gel of cellulose or crosslinked polysaccharide with a sulfating agent in an organic solvent.
Claim 3
Dependent Claim
21 words
The method as claimed in claim 1, wherein the influenza virus-containing solution is an allantoic solution infected with the influenza virus.
Claim 4
Dependent Claim
28 words
The method as claimed in claim 1, wherein the influenza virus-containing solution is one which has been subjected to pretreatment including a treatment with ether to remove lipids.
Claim 5
Dependent Claim
37 words
The method as claimed in of claim 1, wherein the sulfuric acid ester of a crosslinked poly-saccharide is selected from the group consisting of a cross-linked cellulose sulfate, a crosslinked agarose sulfate and a crosslinked dextran sulfate.
Claim 6
Dependent Claim
32 words
The method as claimed in claim 1, wherein the sulfuric acid ester of cellulose is a sulfuric acid ester of crystalline cellulose or a cellulose having a crystalline area and non-crystalline area.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-02-08 | 1991-08-10 | 1992-02-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-02-08 | 1995-08-10 | 1996-02-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-02-08 | 1999-08-10 | 2000-02-09 | $7700.00 | Window Closed / Lapsed |