US4181713A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Isolation of HBs Ag
Assignee: Merck and Co Inc
•
Filing: 1978-10-30
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Granted: 1980-01-01
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Expires: 1998-10-30
Abstract
Abstract High purity HBsAg is injected into guinea pigs to stimulate formulation of HBsAb-positive serum. Gamma globulins containing HBsAb are separated from the serum and the globulin fraction containing HBsAb is isolated and coupled to a Sepharose gel. HBsAg from HBsAg-positive plasma is adsorbed on the gel by formation of an HBsAb-HBsAg complex. HBsAg is eluted from the column and dialysed.
Inventors
- 👤 William J. McAleer
- 👤 Edward H. Wasmuth
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 13:28
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C07K16/00; C07K16/08; C07K16/081; C07K16/082; A; A61; A61K; A61K39/00; C12; C12N; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; Y; Y10; Y10S; Y10S436/00; Y10S436/82; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
107 words
A method for isolating HB s Ag comprising injecting highly purified HB s Ag antigen into a susceptible animal species to stimulate formation of HB s Ab-positive serum, recovering the serum from the animal, treating the serum to separate a globulin fraction containing HB s Ab, further purifying the HB s Ab in the fraction by chromatographic separation, coupling the purified HB s Ag to a linear polysaccharide in the form of a fixed bed, contacting the HB s Ab in the fixed bed with HB s Ag-positive plasma thereby forming an HB s Ab-HB s Ag immune couplex, and separating HB s Ag from the complex.
Claim 2
Independent Claim
107 words
A method for isolating HB s Ag comprising injecting highly purified HB s Ag antigen into a susceptible animal species to stimulate formation of HB s Ab-positive serum, recovering the serum from the animal, treating the serum to separate a globulin fraction containing HB s Ab, further purifying the HB s Ab in the fraction by chromatographic separation, coupling the purified HB s Ag to a linear polysaccharide in the form of a fixed bed, contacting the HB s Ab in the fixed bed with HB s Ag-positive plasma thereby forming an HB s Ab-HB s Ag immune couplex, and separating HB s Ag from the complex.
Claim 3
Dependent Claim
26 words
A method according to claim 1 wherein the HB s Ag-positive plasma is converted to serum before contacting the HB s Ab in the fixed bed.
References Cited (Backward Citations)
US3951977A
Cited
US3994870A
Cited
US4118479A
Cited
US4118478A
Cited
US3976767A
Cited
US4017360A
Cited
US4024243A
Cited
US4087519A
Cited
US4113712A
Cited
US4138287A
Cited
US4088748A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-12-31 | 1983-07-02 | 1984-01-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-12-31 | 1987-07-02 | 1988-01-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-12-31 | 1991-07-02 | 1992-01-01 | $7700.00 | Window Closed / Lapsed |