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US4717676A
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Vesicles and use thereof in an assay
Assignee: Becton Dickinson and Co
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Filing: 1986-03-03
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Granted: 1988-01-05
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Expires: 2006-03-03
Abstract
Abstract Sacs comprising a compound having a hydrophilic peptide radical are prepared and are believed to have improved stability as a result of hydrogen bonding. The sacs, including a detectable marker and derivatized with a ligand may be used as a tracer in an assay.
Inventors
- 👤 Daniel B. Wagner
- 👤 Uri Piran
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 09:41
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/58; G01N33/585; G01N33/586; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/829
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
55 words
A composition, comprising: a sac, said sac including a detectable marker and being derivatized with a ligand, at least a portion of said sac being formed from a compound having the following formula: X--Y--Z wherein X is a hydrophobic radical; Y is a hydrophilic peptide; and Z is a radical including a non-hydrolyzable polar group.
Claim 2
Dependent Claim
55 words
A composition, comprising: a sac, said sac including a detectable marker and being derivatized with a ligand, at least a portion of said sac being formed from a compound having the following formula: X--Y--Z wherein X is a hydrophobic radical; Y is a hydrophilic peptide; and Z is a radical including a non-hydrolyzable polar group.
Claim 3
Dependent Claim
11 words
The composition of claim 1 wherein the polar group is sulfonate.
Claim 4
Dependent Claim
22 words
The composition of claim 1 wherein the polar group is selected from the group consisting of sulfonate, phosphonate, phosphinate and quaternary amine.
Claim 5
Dependent Claim
59 words
In an assay for an analyte wherein at least one of a binder and analyte is contacted with a tracer, the improvement comprising: effecting the contacting of at least one of the analyte and binder with a tracer comprising the composition of claim 3 wherein the ligand of the tracer is bound by one of the binder and analyte.
Claim 6
Dependent Claim
34 words
A reagent kit for determining an analyte, comprising: a package, said package including a binder for at least one of an analyte and a tracer; and a tracer comprising the composition of claim 3.
Claim 7
Dependent Claim
126 words
The composition of claim 1 wherein the hydrophobic radical X is a radical selected from the group consisting of radicals having structural formulas Ii and III: ##STR2## wherein each of R 1 and R 2 is selected from the groups consisting of substituted and unsubstituted hydrocarbon radicals having at least 11 carbon atoms and each of B 1 and B 2 is selected from the group consisting of --CH 2 --; --C(O)NH--; NH; --C(O)0--; --O--; --S--; T is selected from the group consisting of --NH-- --R 4 --F-- wherein R 4 is a hydrocarbon radical having from 1-3 carbon atoms and F is selected from the group consisting of --S--, --NH--, and --C(O)--, and A is a hydrocarbon radical having from 1 to 4 carbon atoms.
Claim 8
Dependent Claim
59 words
In an assay for an analyte wherein at least one of a binder an analyte is contacted with a tracer, the improvement comprising: effecting the contacting of at least one of the analyte and binder with a tracer comprising the composition of claim 6 wherein the ligand of the tracer is bound by one of the binder and analyte.
Claim 9
Dependent Claim
34 words
A reagent kit for determining an analyte, comprising: a package, said package including a binder for at least one of an analyte and a tracer; and a tracer comprising the composition of claim 6.
Claim 10
Dependent Claim
59 words
In an assay for an analyte wherein at least one of a binder an analyte is contacted with a tracer, the improvement comprising: effecting the contacting of at least one of the analyte and binder with a tracer comprising the composition of claim 1, wherein the ligand of the tracer is bound by one of the binder and analyte.
Claim 11
Dependent Claim
12 words
The assay of claim 9 wherein the detectable marker is a chromogen.
Claim 12
Dependent Claim
11 words
The assay of claim 10 wherein the chromogen is sulforhodamine B.
Claim 13
Dependent Claim
34 words
A reagent kit for determining an analyte, comprising: a package, said package including a binder for at least one of an analyte and a tracer; and a tracer comprising the composition of claim 1.
Claim 14
Dependent Claim
13 words
The reagent kit of claim 12 wherein the detectable marker is a chromogen.
Claim 15
Dependent Claim
12 words
The reagent kit of claim 13 wherein the chromogen is sulforhodamine B.
References Cited (Backward Citations)
US4483929A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-01-04 | 1991-07-06 | 1992-01-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-01-04 | 1995-07-06 | 1996-01-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-01-04 | 1999-07-06 | 2000-01-05 | $7700.00 | Window Closed / Lapsed |